LOCUS AB451403 678 bp mRNA linear HUM 02-SEP-2008 DEFINITION Homo sapiens TXNDC9 mRNA for ATP binding protein associated with cell differentiation, partial cds, clone: FLJ08106AAAF. ACCESSION AB451403 VERSION AB451403.1 KEYWORDS Gateway cloning system. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 678) AUTHORS Kawamura,Y., Nomura,N. and Goshima,N. TITLE Direct Submission JOURNAL Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases. Contact:Naoki Goshima Biomedicinal Information Research Center (BIRC), National Institute of Advanced Industrial Science and Technology (AIST); 2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan REFERENCE 2 AUTHORS Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R., Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T., Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C., Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M., Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K., Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A., Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y., Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T., Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H., Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T., Imai,J.-I., Watanabe,S. and Nomura,N. TITLE Human Protein Factory: an infrastructure to convert the human transcriptome into the in vitro-expressed human proteome of versatile utility JOURNAL Unpublished (2008) COMMENT This human Gateway entry clone was constructed by recombining an attB-attached open reading frame (ORF) fragment with attP sequences of the Gateway donor vector pDONR201. The ORF sequence in the entry clone is flanked with attL1 sequence (100 nt) - spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) - spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the translational initiation codon (ATG) and is also flanked with spacer nucleotides (TATG) - attL2 sequence (99 nt) downstream of the ORF. This is an F-type clone that deletes the stop codon for C-terminal tagged proteins. DNA sequences of the entire ORF and flanking regions were validated by sequencing. Clone structure of the ORF and flanking sequences is as follows: 5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3' TATG acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaac gaacaggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL sequences are described in lower cases). Clone information: http://www.HGPD.jp/ This clone was produced in the "Functional Analysis of Protein and Research Application" project supported by the New Energy and Industrial Technology Development Organization (NEDO), Japan FEATURES Location/Qualifiers source 1..678 /clone="FLJ08106AAAF" /db_xref="H-InvDB:HIT000487616" /db_xref="taxon:9606" /mol_type="mRNA" /note="Vector: pDONR201" /organism="Homo sapiens" CDS 1..>678 /codon_start=1 /gene="TXNDC9" /product="ATP binding protein associated with cell differentiation" /protein_id="BAG70217.1" /transl_table=1 /translation="MEADASVDMFSKVLEHQLLQTTKLVEEHLDSEIQKLDQMDEDEL ERLKEKRLQALRKAQQQKQEWLSKGHGEYREIPSERDFFQEVKESENVVCHFYRDSTF RCKILDRHLAILSKKHLETKFLKLNVEKAPFLCERLHIKVIPTLALLKDGKTQDYVVG FTDLGNTDDFTTETLEWRLGSSDILNYSGNLMEPPFQNQKKFGTNFTKLEKKTIRGKK YDSDSDDD" BASE COUNT 246 a 122 c 146 g 164 t ORIGIN 1 atggaagctg atgcatctgt tgacatgttt tccaaagtcc tggagcatca gctgcttcag 61 actaccaaac tggtggaaga acatttggat tctgaaattc aaaaactgga tcagatggat 121 gaggatgaat tggaacgcct taaagaaaag agactccagg cactaaggaa agctcaacag 181 cagaaacaag aatggctttc taaaggacat ggggaataca gagaaatccc tagtgaaaga 241 gacttttttc aagaagtcaa ggagagtgaa aatgtggttt gccatttcta cagagactcc 301 acattcaggt gtaaaatact agacagacat ctggcaatat tgtccaagaa acacctcgag 361 accaaatttt tgaagctgaa tgtggaaaaa gcacctttcc tttgtgagag actgcatatc 421 aaagtcattc ccacactagc actgctaaaa gatgggaaaa cacaagatta tgttgttggg 481 tttactgacc taggaaatac agatgacttc accacagaaa ctttagaatg gaggctcggt 541 tcttctgaca ttcttaatta cagtggaaat ttaatggagc caccatttca gaaccaaaag 601 aaatttggaa caaacttcac aaagctggaa aagaaaacta tccgaggaaa gaaatatgat 661 tcagactctg atgatgat //