LOCUS AB451397 1488 bp mRNA linear HUM 02-SEP-2008
DEFINITION Homo sapiens PRCP mRNA for prolylcarboxypeptidase isoform 1
preproprotein, partial cds, clone: FLJ08097AAAF.
ACCESSION AB451397
VERSION AB451397.1
KEYWORDS Gateway cloning system.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1488)
AUTHORS Kawamura,Y., Nomura,N. and Goshima,N.
TITLE Direct Submission
JOURNAL Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Naoki Goshima
Biomedicinal Information Research Center (BIRC), National
Institute of Advanced Industrial Science and Technology (AIST);
2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan
REFERENCE 2
AUTHORS Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R.,
Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T.,
Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C.,
Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M.,
Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K.,
Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A.,
Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y.,
Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T.,
Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H.,
Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T.,
Imai,J.-I., Watanabe,S. and Nomura,N.
TITLE Human Protein Factory: an infrastructure to convert the human
transcriptome into the in vitro-expressed human proteome of
versatile utility
JOURNAL Unpublished (2008)
COMMENT This human Gateway entry clone was constructed by recombining an
attB-attached open reading frame (ORF) fragment with attP
sequences of the Gateway donor vector pDONR201. The ORF sequence
in the entry clone is flanked with attL1 sequence (100 nt) -
spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) -
spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the
translational initiation codon (ATG) and is also flanked with
spacer nucleotides (TATG) - attL2 sequence (99 nt) downstream of
the ORF. This is an F-type clone that deletes the stop codon for
C-terminal tagged proteins. DNA sequences of the entire ORF and
flanking regions were validated by sequencing. Clone structure of
the ORF and flanking sequences is as follows:
5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct
tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3'
TATG acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaac
gaacaggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence.
attL sequences are described in lower cases).
Clone information: http://www.HGPD.jp/
This clone was produced in the "Functional Analysis of Protein and
Research Application" project supported by the New Energy and
Industrial Technology Development Organization (NEDO), Japan
FEATURES Location/Qualifiers
source 1..1488
/clone="FLJ08097AAAF"
/db_xref="H-InvDB:HIT000487610"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="Vector: pDONR201"
/organism="Homo sapiens"
CDS 1..>1488
/codon_start=1
/gene="PRCP"
/product="prolylcarboxypeptidase isoform 1 preproprotein"
/protein_id="BAG70211.1"
/transl_table=1
/translation="MGRRALLLLLLSFLAPWATIALRPALRALGSLHLPTNPTSLPAV
AKNYSVLYFQQKVDHFGFNTVKTFNQRYLVADKYWKKNGGSILFYTGNEGDIIWFCNN
TGFMWDVAEELKAMLVFAEHRYYGESLPFGDNSFKDSRHLNFLTSEQALADFAELIKH
LKRTIPGAENQPVIAIGGSYGGMLAAWFRMKYPHMVVGALAASAPIWQFEDLVPCGVF
MKIVTTDFRKSGPHCSESIHRSWDAINRLSNTGSGLQWLTGALHLCSPLTSQDIQHLK
DWISETWVNLAMVDYPYASNFLQPLPAWPIKVVCQYLKNPNVSDSLLLQNIFQALNVY
YNYSGQVKCLNISETATSSLGTLGWSYQACTEVVMPFCTNGVDDMFEPHSWNLKELSD
DCFQQWGVRPRPSWITTMYGGKNISSHTNIVFSNGELDPWSGGGVTKDITDTLVAVTI
SEGAHHLDLRTKNALDPMSVLLARSLEVRHMKNWIRDFYDSAGKQH"
BASE COUNT 387 a 345 c 344 g 412 t
ORIGIN
1 atgggccgcc gagccctcct gctcctgctt ctgtcttttc tggcgccctg ggccaccata
61 gccctccggc cggccttaag ggccctcggc agcctacact tgccaaccaa ccccacatcc
121 ctcccggctg tagccaagaa ctattcggtt ctctacttcc aacagaaggt tgatcatttt
181 ggatttaata ctgtgaaaac ttttaatcag cggtacctag tagctgataa atactggaag
241 aaaaatggtg gatcaatact tttctacact ggtaatgaag gggacattat ctggttttgt
301 aataacacgg ggttcatgtg ggatgtggct gaggaactga aagctatgtt ggtgtttgct
361 gaacatcgat actatggaga gtctctcccc tttggtgaca actcattcaa ggattccaga
421 cacttgaatt tcctgacatc agaacaagct ctggctgatt ttgcagagtt aatcaaacac
481 ttgaaaagaa caatcccagg agctgaaaat caacctgtca ttgccatagg aggctcctat
541 ggtggcatgc ttgccgcctg gtttaggatg aaatatcctc atatggtagt tggagctctt
601 gcagcctctg cccctatctg gcagtttgag gatttagtac cttgtggtgt atttatgaag
661 atcgtaacta cagattttag gaaaagcggt ccacattgtt cagagagcat ccaccggtcc
721 tgggatgcca ttaatcgact ctcaaatact ggcagtggtt tgcagtggct tactggagcc
781 cttcacttat gcagcccatt aacttctcag gacatccaac atttgaaaga ctggatctct
841 gaaacctggg tgaatctggc aatggtggac tatccttatg cctctaactt tttacagccc
901 ttgcctgctt ggcctatcaa ggtagtgtgc cagtatttga aaaatcccaa tgtatctgat
961 tcactgctgc tgcagaatat tttccaagct ctgaatgtat attacaatta ttcgggccag
1021 gtgaaatgcc tgaatatttc agagacagca actagcagtc tgggaacact gggttggagc
1081 tatcaggcct gcacagaagt agtcatgccc ttttgtacta atggtgtcga tgacatgttt
1141 gaacctcact catggaactt aaaggaactt tctgatgact gttttcaaca gtggggtgtg
1201 agaccaaggc cctcctggat cactactatg tatggaggca aaaacattag ttcacacaca
1261 aacattgttt tcagcaatgg tgaactagac ccctggtcag gaggtggagt aactaaggat
1321 atcacagaca ctctggttgc agtcaccatc tcagaggggg cccaccactt agatctccgc
1381 accaagaatg ccttggatcc tatgtctgtg ctgttagccc gctccttgga agttagacat
1441 atgaagaatt ggatcagaga tttctatgac agtgcaggaa agcagcac
//