LOCUS AB451392 2343 bp mRNA linear HUM 02-SEP-2008 DEFINITION Homo sapiens CTNNB1 mRNA for catenin beta-1, partial cds, clone: FLJ08084AAAF. ACCESSION AB451392 VERSION AB451392.1 KEYWORDS Gateway cloning system. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 2343) AUTHORS Kawamura,Y., Nomura,N. and Goshima,N. TITLE Direct Submission JOURNAL Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases. Contact:Naoki Goshima Biomedicinal Information Research Center (BIRC), National Institute of Advanced Industrial Science and Technology (AIST); 2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan REFERENCE 2 AUTHORS Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R., Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T., Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C., Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M., Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K., Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A., Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y., Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T., Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H., Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T., Imai,J.-I., Watanabe,S. and Nomura,N. TITLE Human Protein Factory: an infrastructure to convert the human transcriptome into the in vitro-expressed human proteome of versatile utility JOURNAL Unpublished (2008) COMMENT This human Gateway entry clone was constructed by recombining an attB-attached open reading frame (ORF) fragment with attP sequences of the Gateway donor vector pDONR201. The ORF sequence in the entry clone is flanked with attL1 sequence (100 nt) - spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) - spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the translational initiation codon (ATG) and is also flanked with spacer nucleotides (TATG) - attL2 sequence (99 nt) downstream of the ORF. This is an F-type clone that deletes the stop codon for C-terminal tagged proteins. DNA sequences of the entire ORF and flanking regions were validated by sequencing. Clone structure of the ORF and flanking sequences is as follows: 5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3' TATG acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaac gaacaggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL sequences are described in lower cases). Clone information: http://www.HGPD.jp/ This clone was produced in the "Functional Analysis of Protein and Research Application" project supported by the New Energy and Industrial Technology Development Organization (NEDO), Japan FEATURES Location/Qualifiers source 1..2343 /clone="FLJ08084AAAF" /db_xref="H-InvDB:HIT000487605" /db_xref="taxon:9606" /mol_type="mRNA" /note="Vector: pDONR201" /organism="Homo sapiens" CDS 1..>2343 /codon_start=1 /gene="CTNNB1" /product="catenin beta-1" /protein_id="BAG70206.1" /transl_table=1 /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAP SLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPET LDEGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLL NDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTL HNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLA GGLQKMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYE KLLWTTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAA TKQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRT VLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIK ATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRM EEIVEGCTGALHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQD KEAAEAMEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSL FRTEPMAWNETADLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMG GHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL" BASE COUNT 603 a 542 c 584 g 614 t ORIGIN 1 atggctactc aagctgattt gatggagttg gacatggcca tggaaccaga cagaaaagcg 61 gctgttagtc actggcagca acagtcttac ctggactctg gaatccattc tggtgccact 121 accacagctc cttctctgag tggtaaaggc aatcctgagg aagaggatgt ggatacctcc 181 caagtcctgt atgagtggga acagggattt tctcagtcct tcactcaaga acaagtagct 241 gatattgatg gacagtatgc aatgactcga gctcagaggg tacgagctgc tatgttccct 301 gagacattag atgagggcat gcagatccca tctacacagt ttgatgctgc tcatcccact 361 aatgtccagc gtttggctga accatcacag atgctgaaac atgcagttgt aaacttgatt 421 aactatcaag atgatgcaga acttgccaca cgtgcaatcc ctgaactgac aaaactgcta 481 aatgacgagg accaggtggt ggttaataag gctgcagtta tggtccatca gctttctaaa 541 aaggaagctt ccagacacgc tatcatgcgt tctcctcaga tggtgtctgc tattgtacgt 601 accatgcaga atacaaatga tgtagaaaca gctcgttgta ctgctgggac cttgcataac 661 ctttcccatc atcgtgaggg cttactggcc atctttaagt ctggaggcat tcctgccctg 721 gtgaaaatgc ttggttcacc agtggattct gtgttgtttt atgccattac aactctccac 781 aaccttttat tacatcaaga aggagctaaa atggcagtgc gtttagctgg tgggctgcag 841 aaaatggttg ccttgctcaa caaaacaaat gttaaattct tggctattac gacagactgc 901 cttcaaattt tagcttatgg caaccaagaa agcaagctca tcatactggc tagtggtgga 961 ccccaagctt tagtaaatat aatgaggacc tatacttacg aaaaactact gtggaccaca 1021 agcagagtgc tgaaggtgct atctgtctgc tctagtaata agccggctat tgtagaagct 1081 ggtggaatgc aagctttagg acttcacctg acagatccaa gtcaacgtct tgttcagaac 1141 tgtctttgga ctctcaggaa tctttcagat gctgcaacta aacaggaagg gatggaaggt 1201 ctccttggga ctcttgttca gcttctgggt tcagatgata taaatgtggt cacctgtgca 1261 gctggaattc tttctaacct cacttgcaat aattataaga acaagatgat ggtctgccaa 1321 gtgggtggta tagaggctct tgtgcgtact gtccttcggg ctggtgacag ggaagacatc 1381 actgagcctg ccatctgtgc tcttcgtcat ctgaccagcc gacaccaaga agcagagatg 1441 gcccagaatg cagttcgcct tcactatgga ctaccagttg tggttaagct cttacaccca 1501 ccatcccact ggcctctgat aaaggctact gttggattga ttcgaaatct tgccctttgt 1561 cccgcaaatc atgcaccttt gcgtgagcag ggtgccattc cacgactagt tcagttgctt 1621 gttcgtgcac atcaggatac ccagcgccgt acgtccatgg gtgggacaca gcagcaattt 1681 gtggaggggg tccgcatgga agaaatagtt gaaggttgta ccggagccct tcacatccta 1741 gctcgggatg ttcacaaccg aattgttatc agaggactaa ataccattcc attgtttgtg 1801 cagctgcttt attctcccat tgaaaacatc caaagagtag ctgcaggggt cctctgtgaa 1861 cttgctcagg acaaggaagc tgcagaagct atggaagctg agggagccac agctcctctg 1921 acagagttac ttcactctag gaatgaaggt gtggcgacat atgcagctgc tgttttgttc 1981 cgaatgtctg aggacaagcc acaagattac aagaaacggc tttcagttga gctgaccagc 2041 tctctcttca gaacagagcc aatggcttgg aatgagactg ctgatcttgg acttgatatt 2101 ggtgcccagg gagaacccct tggatatcgc caggatgatc ctagctatcg ttcttttcac 2161 tctggtggat atggccagga tgccttgggt atggacccca tgatggaaca tgagatgggt 2221 ggccaccacc ctggtgctga ctatccagtt gatgggctgc cagatctggg gcatgcccag 2281 gacctcatgg atgggctgcc tccaggtgac agcaatcagc tggcctggtt tgatactgac 2341 ctg //