LOCUS AB451390 1392 bp mRNA linear HUM 02-SEP-2008
DEFINITION Homo sapiens HNRNPK mRNA for heterogeneous nuclear
ribonucleoprotein K isoform a, partial cds, clone: FLJ08080AAAF.
ACCESSION AB451390
VERSION AB451390.1
KEYWORDS Gateway cloning system.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1392)
AUTHORS Kawamura,Y., Nomura,N. and Goshima,N.
TITLE Direct Submission
JOURNAL Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Naoki Goshima
Biomedicinal Information Research Center (BIRC), National
Institute of Advanced Industrial Science and Technology (AIST);
2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan
REFERENCE 2
AUTHORS Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R.,
Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T.,
Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C.,
Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M.,
Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K.,
Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A.,
Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y.,
Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T.,
Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H.,
Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T.,
Imai,J.-I., Watanabe,S. and Nomura,N.
TITLE Human Protein Factory: an infrastructure to convert the human
transcriptome into the in vitro-expressed human proteome of
versatile utility
JOURNAL Unpublished (2008)
COMMENT This human Gateway entry clone was constructed by recombining an
attB-attached open reading frame (ORF) fragment with attP
sequences of the Gateway donor vector pDONR201. The ORF sequence
in the entry clone is flanked with attL1 sequence (100 nt) -
spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) -
spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the
translational initiation codon (ATG) and is also flanked with
spacer nucleotides (TATG) - attL2 sequence (99 nt) downstream of
the ORF. This is an F-type clone that deletes the stop codon for
C-terminal tagged proteins. DNA sequences of the entire ORF and
flanking regions were validated by sequencing. Clone structure of
the ORF and flanking sequences is as follows:
5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct
tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3'
TATG acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaac
gaacaggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence.
attL sequences are described in lower cases).
Clone information: http://www.HGPD.jp/
This clone was produced in the "Functional Analysis of Protein and
Research Application" project supported by the New Energy and
Industrial Technology Development Organization (NEDO), Japan
FEATURES Location/Qualifiers
source 1..1392
/clone="FLJ08080AAAF"
/db_xref="H-InvDB:HIT000487603"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="Vector: pDONR201"
/organism="Homo sapiens"
CDS 1..>1392
/codon_start=1
/gene="HNRNPK"
/product="heterogeneous nuclear ribonucleoprotein K
isoform a"
/protein_id="BAG70204.1"
/transl_table=1
/translation="METEQPEETFPNTETNGEFGKRPAEDMEEEQAFKRSRNTDEMVE
LRILLQSKNAGAVIGKGGKNIKALRTDYNASVSVPDSSGPERILSISADIETIGEILK
KIIPTLEEGLQLPSPTATSQLPLESDAVECLNYQHYKGSDFDCELRLLIHQSLAGGII
GVKGAKIKELRENTQTTIKLFQECCPHSTDRVVLIGGKPDRVVECIKIILDLISESPI
KGRAQPYDPNFYDETYDYGGFTMMFDDRRGRPVGFPMRGRGGFDRMPPGRGGRPMPPS
RRDYDDMSPRRGPPPPPPGRGGRGGSRARNLPLPPPPPPRGGDLMAYDRRGRPGDRYD
GMVGFSADETWDSAIDTWSPSEWQMAYEPQGGSGYDYSYAGGRGSYGDLGGPIITTQV
TIPKDLAGSIIGKGGQRIKQIRHESGASIKIDEPLEGSEDRIITITGTQDQIQNAQYL
LQNSVKQYADVEGF"
BASE COUNT 400 a 302 c 356 g 334 t
ORIGIN
1 atggaaactg aacagccaga agaaaccttc cctaacactg aaaccaatgg tgaatttggt
61 aaacgccctg cagaagatat ggaagaggaa caagcattta aaagatctag aaacactgat
121 gagatggttg aattacgcat tctgcttcag agcaagaatg ctggggcagt gattggaaaa
181 ggaggcaaga atattaaggc tctccgtaca gactacaatg ccagtgtttc agtcccagac
241 agcagtggcc ccgagcgcat attgagtatc agtgctgata ttgaaacaat tggagaaatt
301 ctgaagaaaa tcatccctac cttggaagag ggcctgcagt tgccatcacc cactgcaacc
361 agccagctcc cgctcgaatc tgatgctgtg gaatgcttaa attaccaaca ctataaagga
421 agtgactttg actgcgagtt gaggctgttg attcatcaga gtctagcagg aggaattatt
481 ggggtcaaag gtgctaaaat caaagaactt cgagagaaca ctcaaaccac catcaagctt
541 ttccaggaat gctgtcctca ttccactgac agagttgttc ttattggagg aaaacccgat
601 agggttgtag agtgcataaa gatcatcctt gatcttatat ctgagtctcc catcaaagga
661 cgtgcacagc cttatgatcc caatttttac gatgaaacct atgattatgg tggttttaca
721 atgatgtttg atgaccgtcg cggacgccca gtgggatttc ccatgcgggg aagaggtggt
781 tttgacagaa tgcctcctgg tcggggtggg cgtcccatgc ctccatctag aagagattat
841 gatgatatga gccctcgtcg aggaccacct ccccctcctc ccggacgagg cggccggggt
901 ggtagcagag ctcggaatct tcctcttcct ccaccaccac cacctagagg gggagacctc
961 atggcctatg acagaagagg gagacctgga gaccgttacg acggcatggt tggtttcagt
1021 gctgatgaaa cttgggactc tgcaatagat acatggagcc catcagaatg gcagatggct
1081 tatgaaccac agggtggctc cggatatgat tattcctatg cagggggtcg tggctcatat
1141 ggtgatcttg gtggacctat tattactaca caagtaacta ttcccaaaga tttggctgga
1201 tctattattg gcaaaggtgg tcagcggatt aaacaaatcc gtcatgagtc gggagcttcg
1261 atcaaaattg atgagccttt agaaggatcc gaagatcgga tcattaccat tacaggaaca
1321 caggaccaga tacagaatgc acagtatttg ctgcagaaca gtgtgaagca gtatgcagat
1381 gttgaaggat tc
//