LOCUS AB451386 738 bp mRNA linear HUM 02-SEP-2008 DEFINITION Homo sapiens YWHAB mRNA for 14-3-3 protein beta/alpha, partial cds, clone: FLJ08074AAAF. ACCESSION AB451386 VERSION AB451386.1 KEYWORDS Gateway cloning system. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 738) AUTHORS Kawamura,Y., Nomura,N. and Goshima,N. TITLE Direct Submission JOURNAL Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases. Contact:Naoki Goshima Biomedicinal Information Research Center (BIRC), National Institute of Advanced Industrial Science and Technology (AIST); 2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan REFERENCE 2 AUTHORS Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R., Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T., Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C., Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M., Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K., Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A., Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y., Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T., Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H., Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T., Imai,J.-I., Watanabe,S. and Nomura,N. TITLE Human Protein Factory: an infrastructure to convert the human transcriptome into the in vitro-expressed human proteome of versatile utility JOURNAL Unpublished (2008) COMMENT This human Gateway entry clone was constructed by recombining an attB-attached open reading frame (ORF) fragment with attP sequences of the Gateway donor vector pDONR201. The ORF sequence in the entry clone is flanked with attL1 sequence (100 nt) - spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) - spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the translational initiation codon (ATG) and is also flanked with spacer nucleotides (TATG) - attL2 sequence (99 nt) downstream of the ORF. This is an F-type clone that deletes the stop codon for C-terminal tagged proteins. DNA sequences of the entire ORF and flanking regions were validated by sequencing. Clone structure of the ORF and flanking sequences is as follows: 5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3' TATG acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaac gaacaggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL sequences are described in lower cases). Clone information: http://www.HGPD.jp/ This clone was produced in the "Functional Analysis of Protein and Research Application" project supported by the New Energy and Industrial Technology Development Organization (NEDO), Japan FEATURES Location/Qualifiers source 1..738 /clone="FLJ08074AAAF" /db_xref="H-InvDB:HIT000487599" /db_xref="taxon:9606" /mol_type="mRNA" /note="Vector: pDONR201" /organism="Homo sapiens" CDS 1..>738 /codon_start=1 /gene="YWHAB" /product="14-3-3 protein beta/alpha" /protein_id="BAG70200.1" /transl_table=1 /translation="MTMDKSELVQKAKLAEQAERYDDMAAAMKAVTEQGHELSNEERN LLSVAYKNVVGARRSSWRVISSIEQKTERNEKKQQMGKEYREKIEAELQDICNDVLEL LDKYLIPNATQPESKVFYLKMKGDYFRYLSEVASGDNKQTTVSNSQQAYQEAFEISKK EMQPTHPIRLGLALNFSVFYYEILNSPEKACSLAKMAFDEAIAELDTLNEESYKDSTL IMQLLRDNLTLWTSENQGDEGDAGEGEN" BASE COUNT 240 a 147 c 188 g 163 t ORIGIN 1 atgacaatgg ataaaagtga gctggtacag aaagccaaac tcgctgagca ggctgagcga 61 tatgatgata tggctgcagc catgaaggca gtcacagaac aggggcatga actctccaac 121 gaagagagaa atctgctctc tgttgcctac aagaatgtgg taggcgcccg ccgctcttcc 181 tggcgtgtca tctccagcat tgagcagaaa acagagagga atgagaagaa gcagcagatg 241 ggcaaagagt accgtgagaa gatagaggca gaactgcagg acatctgcaa tgatgttctg 301 gagctgttgg acaaatatct tattcccaat gctacacaac cagaaagtaa ggtgttctac 361 ttgaaaatga aaggagatta ttttaggtat ctttctgaag tggcatctgg agacaacaaa 421 caaaccactg tgtcgaactc ccagcaggct taccaggaag catttgaaat tagtaagaaa 481 gaaatgcagc ctacacaccc aattcgtctt ggtctggcac taaatttctc agtcttttac 541 tatgagattc taaactctcc tgaaaaggcc tgtagcctgg caaaaatggc atttgatgaa 601 gcaattgctg aattggatac gctgaatgaa gagtcttata aagacagcac tctgattatg 661 cagttactta gggacaatct cactctgtgg acatcggaaa accagggaga cgaaggagac 721 gctggggagg gagagaac //