LOCUS AB451369 675 bp mRNA linear HUM 02-SEP-2008 DEFINITION Homo sapiens BCAS2 mRNA for breast carcinoma amplified sequence 2, partial cds, clone: FLJ08051AAAF. ACCESSION AB451369 VERSION AB451369.1 KEYWORDS Gateway cloning system. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 675) AUTHORS Kawamura,Y., Nomura,N. and Goshima,N. TITLE Direct Submission JOURNAL Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases. Contact:Naoki Goshima Biomedicinal Information Research Center (BIRC), National Institute of Advanced Industrial Science and Technology (AIST); 2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan REFERENCE 2 AUTHORS Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R., Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T., Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C., Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M., Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K., Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A., Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y., Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T., Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H., Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T., Imai,J.-I., Watanabe,S. and Nomura,N. TITLE Human Protein Factory: an infrastructure to convert the human transcriptome into the in vitro-expressed human proteome of versatile utility JOURNAL Unpublished (2008) COMMENT This human Gateway entry clone was constructed by recombining an attB-attached open reading frame (ORF) fragment with attP sequences of the Gateway donor vector pDONR201. The ORF sequence in the entry clone is flanked with attL1 sequence (100 nt) - spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) - spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the translational initiation codon (ATG) and is also flanked with spacer nucleotides (TATG) - attL2 sequence (99 nt) downstream of the ORF. This is an F-type clone that deletes the stop codon for C-terminal tagged proteins. DNA sequences of the entire ORF and flanking regions were validated by sequencing. Clone structure of the ORF and flanking sequences is as follows: 5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3' TATG acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaac gaacaggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL sequences are described in lower cases). Clone information: http://www.HGPD.jp/ This clone was produced in the "Functional Analysis of Protein and Research Application" project supported by the New Energy and Industrial Technology Development Organization (NEDO), Japan FEATURES Location/Qualifiers source 1..675 /clone="FLJ08051AAAF" /db_xref="H-InvDB:HIT000487582" /db_xref="taxon:9606" /mol_type="mRNA" /note="Vector: pDONR201" /organism="Homo sapiens" CDS 1..>675 /codon_start=1 /gene="BCAS2" /product="breast carcinoma amplified sequence 2" /protein_id="BAG70183.1" /transl_table=1 /translation="MAGTGLVAGEVVVDALPYFDQGYEAPGVREAAAALVEEETRRYR PTKNYLSYLTAPDYSAFETDIMRNEFERLAARQPIELLSMKRYELPAPSSGQKNDITA WQECVNNSMAQLEHQAVRIENLELMSQHGCNAWKVYNENLVHMIEHAQKELQKLRKHI QDLNWQRKNMQLTAGSKLREMESNWVSLVSKNYEIERTIVQLENEIYQIKQQHGEANK ENIRQDF" BASE COUNT 227 a 126 c 166 g 156 t ORIGIN 1 atggcgggca caggtttggt ggctggagag gttgtggtgg atgcgctgcc gtattttgat 61 caaggttatg aagcccctgg tgtgcgggaa gcggctgcag cgctggtgga ggaggaaact 121 cgcagatacc ggcctactaa gaactacctg agctacctga cagccccgga ttattctgcc 181 tttgaaactg acataatgag aaatgaattt gaaagactgg ctgctcgaca accaattgaa 241 ttgctcagta tgaaacgata tgagcttcca gccccctcct ctggtcaaaa aaatgacatt 301 actgcatggc aagaatgtgt aaacaattct atggcccagt tagagcatca agcagttaga 361 attgagaatc tggaactaat gtcacagcat ggatgtaatg cctggaaagt atacaatgaa 421 aatctagttc atatgattga acacgcacag aaggaacttc agaagttaag aaaacatatt 481 caagatttaa actggcagag aaagaacatg caactcacag ctggatctaa attgagagaa 541 atggagtcaa attgggtatc cctggtcagt aagaattatg agattgaacg gactattgtt 601 cagctagaaa atgaaatcta tcaaattaag cagcaacatg gagaggcaaa caaagaaaac 661 atccggcaag acttc //