LOCUS       AB451366                 738 bp    mRNA    linear   HUM 02-SEP-2008
DEFINITION  Homo sapiens TFAM mRNA for mitochondrial transcription factor A,
            partial cds, clone: FLJ08040AAAF.
ACCESSION   AB451366
VERSION     AB451366.1
KEYWORDS    Gateway cloning system.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Kawamura,Y., Nomura,N. and Goshima,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases.
            Contact:Naoki Goshima
            Biomedicinal Information Research Center (BIRC), National
            Institute of Advanced Industrial Science and Technology (AIST);
            2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan
REFERENCE   2
  AUTHORS   Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R.,
            Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T.,
            Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C.,
            Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M.,
            Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K.,
            Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A.,
            Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y.,
            Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T.,
            Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H.,
            Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T.,
            Imai,J.-I., Watanabe,S. and Nomura,N.
  TITLE     Human Protein Factory: an infrastructure to convert the human
            transcriptome into the in vitro-expressed human proteome of
            versatile utility
  JOURNAL   Unpublished (2008)
COMMENT     This human Gateway entry clone was constructed by recombining an
            attB-attached open reading frame (ORF) fragment with attP
            sequences of the Gateway donor vector pDONR201. The ORF sequence
            in the entry clone is flanked with attL1 sequence (100 nt) -
            spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) -
            spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the
            translational initiation codon (ATG) and is also flanked with
            spacer nucleotides (TATG) - attL2 sequence (99 nt) downstream of
            the ORF. This is an F-type clone that deletes the stop codon for
            C-terminal tagged proteins. DNA sequences of the entire ORF and
            flanking regions were validated by sequencing. Clone structure of
            the ORF and flanking sequences is as follows:
            5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct
            tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3'
            TATG acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaac
            gaacaggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence.
            attL sequences are described in lower cases).
            Clone information: http://www.HGPD.jp/
            This clone was produced in the "Functional Analysis of Protein and
            Research Application" project supported by the New Energy and
            Industrial Technology Development Organization (NEDO), Japan
FEATURES             Location/Qualifiers
     source          1..738
                     /clone="FLJ08040AAAF"
                     /db_xref="H-InvDB:HIT000487579"
                     /db_xref="taxon:9606"
                     /mol_type="mRNA"
                     /note="Vector: pDONR201"
                     /organism="Homo sapiens"
     CDS             1..>738
                     /codon_start=1
                     /gene="TFAM"
                     /product="mitochondrial transcription factor A"
                     /protein_id="BAG70180.1"
                     /transl_table=1
                     /translation="MAFLRSMWGVLTALGRSGAELCTGCGSRLRSPFSFVYLPRWFSS
                     VLASCPKKPVSSYLRFSKEQLPIFKAQNPDAKTTELIRRIAQRWRELPDSKKKIYQDA
                     YRAEWQVYKEEISRFKEQLTPSQIMSLEKEIMDKHLKRKAMTKKKELTLLGKPKRPRS
                     AYNVYVAERFQEAKGDSPQEKLKTVKENWKNLSDSEKELYIQHAKEDETRYHNEMKSW
                     EEQMIEVGRKDLLRRTIKKQRKYGAEEC"
BASE COUNT          264 a          127 c          170 g          177 t
ORIGIN      
        1 atggcgtttc tccgaagcat gtggggcgtg ctgactgccc tgggaaggtc tggagcagag
       61 ctgtgcaccg gctgtggaag tcgactgcgc tcccccttca gttttgtgta tttaccgagg
      121 tggttttcat ctgtcttggc aagttgtcca aagaaacctg taagttctta ccttcgattt
      181 tctaaagaac aactacccat atttaaagct cagaacccag atgcaaaaac tacagaacta
      241 attagaagaa ttgcccagcg ttggagggaa cttcctgatt caaagaaaaa aatatatcaa
      301 gatgcttata gggcggagtg gcaggtatat aaagaagaga taagcagatt taaagaacag
      361 ctaactccaa gtcagattat gtctttggaa aaagaaatca tggacaaaca tttaaaaagg
      421 aaagctatga caaaaaaaaa agagttaaca ctgcttggaa aaccaaaaag acctcgttca
      481 gcttataacg tttatgtagc tgaaagattc caagaagcta agggtgattc accgcaggaa
      541 aagctgaaga ctgtaaagga aaactggaaa aatctgtctg actctgaaaa ggaattatat
      601 attcagcatg ctaaagagga cgaaactcgt tatcataatg aaatgaagtc ttgggaagaa
      661 caaatgattg aagttggacg aaaggatctt ctacgtcgca caataaagaa acaacgaaaa
      721 tatggtgctg aggagtgt
//