LOCUS AB451366 738 bp mRNA linear HUM 02-SEP-2008 DEFINITION Homo sapiens TFAM mRNA for mitochondrial transcription factor A, partial cds, clone: FLJ08040AAAF. ACCESSION AB451366 VERSION AB451366.1 KEYWORDS Gateway cloning system. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 738) AUTHORS Kawamura,Y., Nomura,N. and Goshima,N. TITLE Direct Submission JOURNAL Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases. Contact:Naoki Goshima Biomedicinal Information Research Center (BIRC), National Institute of Advanced Industrial Science and Technology (AIST); 2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan REFERENCE 2 AUTHORS Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R., Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T., Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C., Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M., Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K., Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A., Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y., Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T., Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H., Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T., Imai,J.-I., Watanabe,S. and Nomura,N. TITLE Human Protein Factory: an infrastructure to convert the human transcriptome into the in vitro-expressed human proteome of versatile utility JOURNAL Unpublished (2008) COMMENT This human Gateway entry clone was constructed by recombining an attB-attached open reading frame (ORF) fragment with attP sequences of the Gateway donor vector pDONR201. The ORF sequence in the entry clone is flanked with attL1 sequence (100 nt) - spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) - spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the translational initiation codon (ATG) and is also flanked with spacer nucleotides (TATG) - attL2 sequence (99 nt) downstream of the ORF. This is an F-type clone that deletes the stop codon for C-terminal tagged proteins. DNA sequences of the entire ORF and flanking regions were validated by sequencing. Clone structure of the ORF and flanking sequences is as follows: 5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3' TATG acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaac gaacaggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL sequences are described in lower cases). Clone information: http://www.HGPD.jp/ This clone was produced in the "Functional Analysis of Protein and Research Application" project supported by the New Energy and Industrial Technology Development Organization (NEDO), Japan FEATURES Location/Qualifiers source 1..738 /clone="FLJ08040AAAF" /db_xref="H-InvDB:HIT000487579" /db_xref="taxon:9606" /mol_type="mRNA" /note="Vector: pDONR201" /organism="Homo sapiens" CDS 1..>738 /codon_start=1 /gene="TFAM" /product="mitochondrial transcription factor A" /protein_id="BAG70180.1" /transl_table=1 /translation="MAFLRSMWGVLTALGRSGAELCTGCGSRLRSPFSFVYLPRWFSS VLASCPKKPVSSYLRFSKEQLPIFKAQNPDAKTTELIRRIAQRWRELPDSKKKIYQDA YRAEWQVYKEEISRFKEQLTPSQIMSLEKEIMDKHLKRKAMTKKKELTLLGKPKRPRS AYNVYVAERFQEAKGDSPQEKLKTVKENWKNLSDSEKELYIQHAKEDETRYHNEMKSW EEQMIEVGRKDLLRRTIKKQRKYGAEEC" BASE COUNT 264 a 127 c 170 g 177 t ORIGIN 1 atggcgtttc tccgaagcat gtggggcgtg ctgactgccc tgggaaggtc tggagcagag 61 ctgtgcaccg gctgtggaag tcgactgcgc tcccccttca gttttgtgta tttaccgagg 121 tggttttcat ctgtcttggc aagttgtcca aagaaacctg taagttctta ccttcgattt 181 tctaaagaac aactacccat atttaaagct cagaacccag atgcaaaaac tacagaacta 241 attagaagaa ttgcccagcg ttggagggaa cttcctgatt caaagaaaaa aatatatcaa 301 gatgcttata gggcggagtg gcaggtatat aaagaagaga taagcagatt taaagaacag 361 ctaactccaa gtcagattat gtctttggaa aaagaaatca tggacaaaca tttaaaaagg 421 aaagctatga caaaaaaaaa agagttaaca ctgcttggaa aaccaaaaag acctcgttca 481 gcttataacg tttatgtagc tgaaagattc caagaagcta agggtgattc accgcaggaa 541 aagctgaaga ctgtaaagga aaactggaaa aatctgtctg actctgaaaa ggaattatat 601 attcagcatg ctaaagagga cgaaactcgt tatcataatg aaatgaagtc ttgggaagaa 661 caaatgattg aagttggacg aaaggatctt ctacgtcgca caataaagaa acaacgaaaa 721 tatggtgctg aggagtgt //