LOCUS AB451344 1095 bp mRNA linear HUM 02-SEP-2008 DEFINITION Homo sapiens MAPK13 mRNA for mitogen-activated protein kinase 13, partial cds, clone: FLJ08003AAAF. ACCESSION AB451344 VERSION AB451344.1 KEYWORDS Gateway cloning system. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1095) AUTHORS Kawamura,Y., Nomura,N. and Goshima,N. TITLE Direct Submission JOURNAL Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases. Contact:Naoki Goshima Biomedicinal Information Research Center (BIRC), National Institute of Advanced Industrial Science and Technology (AIST); 2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan REFERENCE 2 AUTHORS Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R., Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T., Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C., Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M., Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K., Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A., Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y., Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T., Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H., Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T., Imai,J.-I., Watanabe,S. and Nomura,N. TITLE Human Protein Factory: an infrastructure to convert the human transcriptome into the in vitro-expressed human proteome of versatile utility JOURNAL Unpublished (2008) COMMENT This human Gateway entry clone was constructed by recombining an attB-attached open reading frame (ORF) fragment with attP sequences of the Gateway donor vector pDONR201. The ORF sequence in the entry clone is flanked with attL1 sequence (100 nt) - spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) - spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the translational initiation codon (ATG) and is also flanked with spacer nucleotides (TATG) - attL2 sequence (99 nt) downstream of the ORF. This is an F-type clone that deletes the stop codon for C-terminal tagged proteins. DNA sequences of the entire ORF and flanking regions were validated by sequencing. Clone structure of the ORF and flanking sequences is as follows: 5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3' TATG acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaac gaacaggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL sequences are described in lower cases). Clone information: http://www.HGPD.jp/ This clone was produced in the "Functional Analysis of Protein and Research Application" project supported by the New Energy and Industrial Technology Development Organization (NEDO), Japan FEATURES Location/Qualifiers source 1..1095 /clone="FLJ08003AAAF" /db_xref="H-InvDB:HIT000487557" /db_xref="taxon:9606" /mol_type="mRNA" /note="Vector: pDONR201" /organism="Homo sapiens" CDS 1..>1095 /codon_start=1 /gene="MAPK13" /product="mitogen-activated protein kinase 13" /protein_id="BAG70158.1" /transl_table=1 /translation="MSLIRKKGFYKQDVNKTAWELPKTYVSPTHVGSGAYDSVCSAID KRSGEKVAIKKLSRPFQSEIFAKRAYRELLLLKHMQHENVIGLLDVFTPASSLRNFYD FYLVMPFMQTDLQKIMGMEFSEEKIQYLVYQMLKGLKYIHSAGVVHRDLKPGNLAVNE DCELKILDFGLARHADAEMTGYVVTRWYRAPEVILSWMHYNQTVDIWSVGCIMAEMLT GKTLFKGKDYLDQLTQILKVTGVPGTEFVQKLNDKAAKSYIQSLPQTPRKDFTQLFPR ASPQAADLLEKMLELDVDKRLTAAQALTHPFFEPFRDPEEETEAQQPFDDSLEHEKLT VDEWKQHIYKEIVNFSPIARKDSRRRSGMKL" BASE COUNT 260 a 310 c 322 g 203 t ORIGIN 1 atgagcctca tccggaaaaa gggcttctac aagcaggacg tcaacaagac cgcctgggag 61 ctgcccaaga cctacgtgtc cccgacgcac gtcggcagcg gggcctatga ctccgtgtgc 121 tcggccatcg acaagcggtc aggggagaag gtggccatca agaagctgag ccgacccttt 181 cagtccgaga tcttcgccaa gcgcgcctac cgggagctgc tgctgctgaa gcacatgcag 241 catgagaacg tcattgggct cctggatgtc ttcaccccag cctcctccct gcgcaacttc 301 tatgacttct acctggtgat gcccttcatg cagacggatc tgcagaagat catggggatg 361 gagttcagtg aggagaagat ccagtacctg gtgtatcaga tgctcaaagg ccttaagtac 421 atccactctg ctggggtcgt gcacagggac ctgaagccag gcaacctggc tgtgaatgag 481 gactgtgaac tgaagattct ggattttggg ctggcgcgac atgcagacgc cgagatgact 541 ggctacgtgg tgacccgctg gtaccgagcc cccgaggtga tcctcagctg gatgcactac 601 aaccagacag tggacatctg gtctgtgggc tgtatcatgg cagagatgct gacagggaaa 661 actctgttca aggggaaaga ttacctggac cagctgaccc agatcctgaa agtgaccggg 721 gtgcctggca cggagtttgt gcagaagctg aacgacaaag cggccaaatc ctacatccag 781 tccctgccac agacccccag gaaggatttc actcagctgt tcccacgggc cagcccccag 841 gctgcggacc tgctggagaa gatgctggag ctagacgtgg acaagcgcct gacggccgcg 901 caggccctca cccatccctt ctttgaaccc ttccgggacc ctgaggaaga gacggaggcc 961 cagcagccgt ttgatgattc cttagaacac gagaaactca cagtggatga atggaagcag 1021 cacatctaca aggagattgt gaacttcagc cccattgccc ggaaggactc acggcgccgg 1081 agtggcatga agctg //