LOCUS AB451343 1986 bp mRNA linear HUM 02-SEP-2008 DEFINITION Homo sapiens DDX3X mRNA for ATP-dependent RNA helicase DDX3X, partial cds, clone: FLJ08002AAAF. ACCESSION AB451343 VERSION AB451343.1 KEYWORDS Gateway cloning system. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1986) AUTHORS Kawamura,Y., Nomura,N. and Goshima,N. TITLE Direct Submission JOURNAL Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases. Contact:Naoki Goshima Biomedicinal Information Research Center (BIRC), National Institute of Advanced Industrial Science and Technology (AIST); 2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan REFERENCE 2 AUTHORS Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R., Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T., Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C., Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M., Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K., Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A., Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y., Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T., Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H., Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T., Imai,J.-I., Watanabe,S. and Nomura,N. TITLE Human Protein Factory: an infrastructure to convert the human transcriptome into the in vitro-expressed human proteome of versatile utility JOURNAL Unpublished (2008) COMMENT This human Gateway entry clone was constructed by recombining an attB-attached open reading frame (ORF) fragment with attP sequences of the Gateway donor vector pDONR201. The ORF sequence in the entry clone is flanked with attL1 sequence (100 nt) - spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) - spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the translational initiation codon (ATG) and is also flanked with spacer nucleotides (TATG) - attL2 sequence (99 nt) downstream of the ORF. This is an F-type clone that deletes the stop codon for C-terminal tagged proteins. DNA sequences of the entire ORF and flanking regions were validated by sequencing. Clone structure of the ORF and flanking sequences is as follows: 5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3' TATG acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaac gaacaggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL sequences are described in lower cases). Clone information: http://www.HGPD.jp/ This clone was produced in the "Functional Analysis of Protein and Research Application" project supported by the New Energy and Industrial Technology Development Organization (NEDO), Japan FEATURES Location/Qualifiers source 1..1986 /clone="FLJ08002AAAF" /db_xref="H-InvDB:HIT000487556" /db_xref="taxon:9606" /mol_type="mRNA" /note="Vector: pDONR201" /organism="Homo sapiens" CDS 1..>1986 /codon_start=1 /gene="DDX3X" /product="ATP-dependent RNA helicase DDX3X" /protein_id="BAG70157.1" /transl_table=1 /translation="MSHVAVENALGLDQQFAGLDLNSSDNQSGGSTASKGRYIPPHLR NREATKGFYDKDSSGWSSSKDKDAYSSFGSRSDSRGKSSFFSDRGSGSRGRFDDRGRS DYDGIGSRGDRSGFGKFERGGNSRWCDKSDEDDWSKPLPPSERLEQELFSGGNTGINF EKYDDIPVGATGNNCPPHIESFSDVEMGEIIMGNIELTRYTRPTPVQKHAIPIIKEKR DLMACAQTGSGKTAAFLLPILSQIYSDGPGEALRAMKENGRYGRRKQYPISLVLAPTR ELAVQIYEEARKFSYRSRVRPCVVYGGADIGQQIRDLERGCHLLVATPGRLVDMMERG KIGLDFCKYLVLDEADRMLDMGFEPQIRRIVEQDTMPPKGVRHTMMFSATFPKEIQML ARDFLDEYIFLAVGRVGSTSENITQKVVWVEESDKRSFLLDLLNATGKDSLTLVFVET KKGADSLEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDIS NVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFNERNINITKDLLDLLVEAKQE VPSWLENMAYEHHYKGSSRGRSKSSRFSGGFGARDYRQSSGASSSSFSSSRASSSRSG GGGHGSSRGFGGGGYGGFYNSDGYGGNYNSQGVDWWGN" BASE COUNT 570 a 369 c 545 g 502 t ORIGIN 1 atgagtcatg tggcagtgga aaatgcgctc gggctggacc agcagtttgc tggcctagac 61 ctgaactctt cagataatca gagtggagga agtacagcca gcaaagggcg ctatattcct 121 cctcatttaa ggaaccgaga agctactaaa ggtttctacg ataaagacag ttcagggtgg 181 agttctagca aagataagga tgcgtatagc agttttggat ctcgtagtga ttcaagaggg 241 aagtctagct tcttcagtga tcgtggaagt ggatcaaggg gaaggtttga tgatcgtgga 301 cggagtgatt acgatggcat tggcagccgt ggtgacagaa gtggctttgg caaatttgaa 361 cgtggtggaa acagtcgctg gtgtgacaaa tcagatgaag atgattggtc aaaaccactc 421 ccaccaagtg aacgcttgga acaggaactc ttttctggag gcaacactgg gattaatttt 481 gagaaatacg atgacattcc agttggggca acaggcaaca actgtcctcc acatattgaa 541 agtttcagtg atgttgagat gggagaaatt atcatgggaa acattgagct tactcgttat 601 actcgcccaa ctccagtgca aaagcatgct attcctatta tcaaagagaa aagagacttg 661 atggcttgtg cccaaacagg gtctggaaaa actgcagcat ttctgttgcc catcttgagt 721 cagatttatt cagatggccc aggcgaggct ttgagggcca tgaaggaaaa tggaaggtat 781 gggcgccgca aacaataccc aatctccttg gtattagcac caacgagaga gttggcagta 841 cagatctacg aggaagccag aaaattttca taccgatcta gagttcgtcc ttgcgtggtt 901 tatggtggtg ccgatattgg tcagcagatt cgagacttgg aacgtggatg ccatttgtta 961 gtagccactc caggacgtct agtggatatg atggaaagag gaaagattgg attagacttt 1021 tgcaaatact tggtgttaga tgaagctgat cggatgttgg atatggggtt tgagcctcag 1081 attcgtagaa tagtcgaaca agatactatg cctccaaagg gtgtccgcca cactatgatg 1141 tttagtgcta cttttcctaa ggaaatacag atgctggctc gtgatttctt agatgaatat 1201 atcttcttgg ctgtaggaag agttggctct acctctgaaa acatcacaca gaaagtagtt 1261 tgggtggaag aatcagacaa acggtcattt ctgcttgacc tcctaaatgc aacaggcaag 1321 gattcactga ccttagtgtt tgtggagacc aaaaagggtg cagattctct ggaggatttc 1381 ttataccatg aaggatacgc atgtaccagc atccatggag accgttctca gagggataga 1441 gaagaggccc ttcaccagtt ccgctcagga aaaagcccaa ttttagtggc tacagcagta 1501 gcagcaagag gactggacat ttcaaatgtg aaacatgtta tcaattttga cttgccaagt 1561 gatattgaag aatatgtaca tcgtattggt cgtacgggac gtgtaggaaa ccttggcctg 1621 gcaacctcat tctttaacga gaggaacata aatattacta aggatttgtt ggatcttctt 1681 gttgaagcta aacaagaagt gccgtcttgg ttagaaaaca tggcttatga acaccactac 1741 aagggtagca gtcgtggacg ttctaagagt agcagattta gtggagggtt tggtgccaga 1801 gactaccgac aaagtagcgg tgccagcagt tccagcttca gcagcagccg cgcaagcagc 1861 agccgcagtg gcggaggtgg ccacggtagc agcagaggat ttggtggagg tggctatgga 1921 ggcttttaca acagtgatgg atatggagga aattataact cccagggggt tgactggtgg 1981 ggtaac //