LOCUS AB451335 1713 bp mRNA linear HUM 02-SEP-2008
DEFINITION Homo sapiens YTHDF2 mRNA for high glucose-regulated protein 8,
complete cds, clone: FLJ80394SAAN.
ACCESSION AB451335
VERSION AB451335.1
KEYWORDS Gateway cloning system.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1713)
AUTHORS Kawamura,Y., Nomura,N. and Goshima,N.
TITLE Direct Submission
JOURNAL Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Naoki Goshima
Biomedicinal Information Research Center (BIRC), National
Institute of Advanced Industrial Science and Technology (AIST);
2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan
REFERENCE 2
AUTHORS Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R.,
Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T.,
Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C.,
Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M.,
Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K.,
Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A.,
Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y.,
Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T.,
Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H.,
Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T.,
Imai,J.-I., Watanabe,S. and Nomura,N.
TITLE Human Protein Factory: an infrastructure to convert the human
transcriptome into the in vitro-expressed human proteome of
versatile utility
JOURNAL Unpublished (2008)
COMMENT This human Gateway entry clone was constructed by recombining an
attB-attached open reading frame (ORF) fragment with attP
sequences of the Gateway donor vector pDONR201. The ORF sequence
in the entry clone is flanked with attL1 sequence (100 nt) -
spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) -
spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the
translational initiation codon (ATG) and is also flanked with a
spacer nucleotide (G) - attL2 sequence (99 nt) downstream of the
ORF. This is an N-type clone which has an intrinsic stop codon at
the end of ORF. DNA sequences of the entire ORF and flanking
regions were validated by sequencing. Clone structure of the ORF
and flanking sequences is as follows:
5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct
tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3'
G acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaacgaa
caggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL
sequences are described in lower cases).
Clone information: http://www.HGPD.jp/
This clone was produced in the "Functional Analysis of Protein and
Research Application" project supported by the New Energy and
Industrial Technology Development Organization (NEDO), Japan
FEATURES Location/Qualifiers
source 1..1713
/clone="FLJ80394SAAN"
/db_xref="H-InvDB:HIT000487548"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="Vector: pDONR201"
/organism="Homo sapiens"
CDS 1..1713
/codon_start=1
/gene="YTHDF2"
/product="high glucose-regulated protein 8"
/protein_id="BAG70149.1"
/transl_table=1
/translation="MSASSLLEQRPKGQGNKVQNGSVHQKDGLNDDDFEPYLSPQARP
NNAYTAMSDSYLPSYYSPSIGFSYSLGEAAWSTGGDTAMPYLTSYGQLSNGEPHFLPD
AMFGQPGALGSTPFLGQHGFNFFPSGIDFSAWGNNSSQGQSTQSSGYSSNYAYAPSSL
GGAMIDGQSAFANETLNKAPGMNTIDQGMAALKLGSTEVASNVPKVVGSAVGSGSITS
NIVASNSLPPATIAPPKPASWADIASKPAKQQPKLKTKNGIAGSSLPPPPIKHNMDIG
TWDNKGPVAKAPSQALVQNIGQPTQGSPQPVGQQANNSPPVAQASVGQQTQPLPPPPP
QPAQLSVQQQAAQPTRWVAPRNRGSGFGHNGVDGNGVGQSQAGSGSTPSEPHPVLEKL
RSINNYNPKDFDWNLKHGRVFIIKSYSEDDIHRSIKYNIWCSTEHGNKRLDAAYRSMN
GKGPVYLLFSVNGSGHFCGVAEMKSAVDYNTCAGVWSQDKWKGRFDVRWIFVKDVPNS
QLRHIRLENNENKPVTNSRDTQEVPLEKAKQVLKIIASYKHTTSIFDDFSHYEKRQRG
RRKC"
BASE COUNT 476 a 415 c 413 g 409 t
ORIGIN
1 atgtcggcca gcagcctctt ggagcagaga ccaaaaggtc aaggaaacaa agtacaaaat
61 ggatctgtac atcaaaagga tggattaaac gatgatgatt ttgaacctta cttgagtcca
121 caggcaaggc ccaataatgc atatactgcc atgtcagatt cctacttacc cagttactac
181 agtccctcca ttggcttctc ctattctttg ggtgaagctg cttggtctac ggggggtgac
241 acagccatgc cctacttaac ttcttatgga cagctgagca acggagagcc ccacttccta
301 ccagatgcaa tgtttgggca accaggagcc ctaggtagca ctccatttct tggtcagcat
361 ggttttaatt tctttcccag tgggattgac ttctcagcat ggggaaataa cagttctcag
421 ggacagtcta ctcagagctc tggatatagt agcaattatg cttatgcacc tagctcctta
481 ggtggagcca tgattgatgg acagtcagct tttgccaatg agaccctcaa taaggctcct
541 ggcatgaata ctatagacca agggatggca gcactgaagt tgggtagcac agaagttgca
601 agcaatgttc caaaagttgt aggttctgct gttggtagcg ggtccattac tagtaacatc
661 gtggcttcca atagtttgcc tccagccacc attgctcctc caaaaccagc atcttgggct
721 gatattgcta gcaagcctgc aaaacagcaa cctaaactga agaccaagaa tggcattgca
781 gggtcaagtc ttccgccacc cccgataaag cataacatgg atattggaac ttgggataac
841 aagggtcccg ttgcaaaagc cccctcacag gctttggttc agaatatagg tcagccaacc
901 caggggtctc ctcagcctgt aggtcagcag gctaacaata gcccaccagt ggctcaggca
961 tcagtagggc aacagacaca gccattgcct ccacctccac cacagcctgc ccagctttca
1021 gtccagcaac aggcagctca gccaacccgc tgggtagcac ctcggaaccg tggcagtggg
1081 ttcggtcata atggggtgga tggtaatgga gtaggacagt ctcaggctgg ttctggatct
1141 actccttcag aaccccaccc agtgttggag aagcttcggt ccattaataa ctataacccc
1201 aaagattttg actggaatct gaaacatggc cgggttttca tcattaagag ctactctgag
1261 gacgatattc accgttccat taagtataat atttggtgca gcacagagca tggtaacaag
1321 agactggatg ctgcttatcg ttccatgaac gggaaaggcc ccgtttactt acttttcagt
1381 gtcaacggca gtggacactt ctgtggcgtg gcagaaatga aatctgctgt ggactacaac
1441 acatgtgcag gtgtgtggtc ccaggacaaa tggaagggtc gttttgatgt caggtggatt
1501 tttgtgaagg acgttcccaa tagccaactg cgacacattc gcctagagaa caacgagaat
1561 aaaccagtga ccaactctag ggacactcag gaagtgcctc tggaaaaggc taagcaggtg
1621 ttgaaaatta tagccagcta caagcacacc acttccattt ttgatgactt ctcacactat
1681 gagaaacgcc aaagaggaag aagaaagtgt taa
//