LOCUS AB451320 1410 bp mRNA linear HUM 02-SEP-2008
DEFINITION Homo sapiens TRAIP mRNA for TRAF interacting protein, complete
cds, clone: FLJ08192AAAN.
ACCESSION AB451320
VERSION AB451320.1
KEYWORDS Gateway cloning system.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1410)
AUTHORS Kawamura,Y., Nomura,N. and Goshima,N.
TITLE Direct Submission
JOURNAL Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Naoki Goshima
Biomedicinal Information Research Center (BIRC), National
Institute of Advanced Industrial Science and Technology (AIST);
2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan
REFERENCE 2
AUTHORS Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R.,
Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T.,
Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C.,
Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M.,
Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K.,
Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A.,
Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y.,
Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T.,
Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H.,
Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T.,
Imai,J.-I., Watanabe,S. and Nomura,N.
TITLE Human Protein Factory: an infrastructure to convert the human
transcriptome into the in vitro-expressed human proteome of
versatile utility
JOURNAL Unpublished (2008)
COMMENT This human Gateway entry clone was constructed by recombining an
attB-attached open reading frame (ORF) fragment with attP
sequences of the Gateway donor vector pDONR201. The ORF sequence
in the entry clone is flanked with attL1 sequence (100 nt) -
spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) -
spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the
translational initiation codon (ATG) and is also flanked with a
spacer nucleotide (G) - attL2 sequence (99 nt) downstream of the
ORF. This is an N-type clone which has an intrinsic stop codon at
the end of ORF. DNA sequences of the entire ORF and flanking
regions were validated by sequencing. Clone structure of the ORF
and flanking sequences is as follows:
5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct
tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3'
G acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaacgaa
caggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL
sequences are described in lower cases).
Clone information: http://www.HGPD.jp/
This clone was produced in the "Functional Analysis of Protein and
Research Application" project supported by the New Energy and
Industrial Technology Development Organization (NEDO), Japan
FEATURES Location/Qualifiers
source 1..1410
/clone="FLJ08192AAAN"
/db_xref="H-InvDB:HIT000487533"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="Vector: pDONR201"
/organism="Homo sapiens"
CDS 1..1410
/codon_start=1
/gene="TRAIP"
/product="TRAF interacting protein"
/protein_id="BAG70134.1"
/transl_table=1
/translation="MPIRALCTICSDFFDHSRDVAAIHCGHTFHLQCLIQWFETAPSR
TCPQCRIQVGKRTIINKLFFDLAQEEENVLDAEFLKNELDNVRAQLSQKDKEKRDSQV
IIDTLRDTLEERNATVVSLQQALGKAEMLCSTLKKQMKYLEQQQDETKQAQEEARRLR
SKMKTMEQIELLLQSQRPEVEEMIRDMGVGQSAVEQLAVYCVSLKKEYENLKEARKAS
GEVADKLRKDLFSSRSKLQTVYSELDQAKLELKSAQKDLQSADKEIMSLKKKLTMLQE
TLNLPPVASETVDRLVLESPAPVEVNLKLRRPSFRDDIDLNATFDVDTPPARPSSSQH
GYYEKLCLEKSHSPIQNVPKKICKGPRKESQLSLGGQSCAGEPDEELVGAFPIFVRNA
ILGQKQPKRPRSESSCSKDVVRTGFDGLGGRTKFIQPTDTVMIRPLPVKPKTKVKQRV
RVKTVPSLFQAKLDTFLWS"
BASE COUNT 377 a 364 c 384 g 285 t
ORIGIN
1 atgcctatcc gtgctctgtg cactatctgc tccgacttct tcgatcactc ccgcgacgtg
61 gccgccatcc actgcggcca caccttccac ttgcagtgct taattcagtg gtttgagaca
121 gcaccaagtc ggacctgccc acagtgccga atccaggttg gcaaaagaac tattatcaat
181 aagctcttct ttgatcttgc ccaggaggag gagaatgtct tggatgcaga attcttaaag
241 aatgaactgg acaatgtcag agcccagctt tcccagaaag acaaggagaa acgagacagc
301 caggtcatca tcgacactct gcgggatacg ctggaagaac gcaatgctac tgtggtatct
361 ctgcagcagg ccttgggcaa ggccgagatg ctgtgctcca cactgaaaaa gcagatgaag
421 tacttagagc agcagcagga tgagaccaaa caagcacaag aggaggcccg ccggctcagg
481 agcaagatga agaccatgga gcagattgag cttctactcc agagccagcg ccctgaggtg
541 gaggagatga tccgagacat gggtgtggga cagtcagcgg tggaacagct ggctgtgtac
601 tgtgtgtctc tcaagaaaga gtacgagaat ctaaaagagg cacggaaggc ctcaggggag
661 gtggctgaca agctgaggaa ggatttgttt tcctccagaa gcaagttgca gacagtctac
721 tctgaattgg atcaggccaa gttagaactg aagtcagccc agaaggactt acagagtgct
781 gacaaggaaa tcatgagcct gaaaaagaag ctaacgatgc tgcaggaaac cttgaacctg
841 ccaccagtgg ccagtgagac tgtcgaccgc ctggttttag agagcccagc ccctgtggag
901 gtgaatctga agctccgccg gccatccttc cgtgatgata ttgatctcaa tgctaccttt
961 gatgtggata ctcccccagc ccggccctcc agctcccagc atggttacta cgaaaaactt
1021 tgcctagaga agtcacactc cccaattcag aatgtcccca agaagatatg caaaggcccc
1081 aggaaggagt cccagctctc actgggtggc cagagctgtg caggagagcc agatgaggaa
1141 ctggttggtg ccttccctat ttttgtccgg aatgccatcc taggccagaa acagcccaag
1201 aggcccaggt cagagtcctc ttgcagcaaa gatgtggtaa ggacaggctt cgatgggctc
1261 ggtggccgga caaaattcat ccagcctact gacacagtca tgatccgccc attgcctgtt
1321 aagcccaaga ccaaggttaa gcagagggtg agggtgaaga cagtgccttc tctcttccag
1381 gccaagctgg acaccttcct gtggtcgtaa
//