LOCUS AB451317 597 bp mRNA linear HUM 02-SEP-2008 DEFINITION Homo sapiens YKT6 mRNA for YKT6 v-SNARE protein, complete cds, clone: FLJ08184AAAN. ACCESSION AB451317 VERSION AB451317.1 KEYWORDS Gateway cloning system. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 597) AUTHORS Kawamura,Y., Nomura,N. and Goshima,N. TITLE Direct Submission JOURNAL Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases. Contact:Naoki Goshima Biomedicinal Information Research Center (BIRC), National Institute of Advanced Industrial Science and Technology (AIST); 2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan REFERENCE 2 AUTHORS Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R., Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T., Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C., Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M., Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K., Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A., Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y., Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T., Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H., Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T., Imai,J.-I., Watanabe,S. and Nomura,N. TITLE Human Protein Factory: an infrastructure to convert the human transcriptome into the in vitro-expressed human proteome of versatile utility JOURNAL Unpublished (2008) COMMENT This human Gateway entry clone was constructed by recombining an attB-attached open reading frame (ORF) fragment with attP sequences of the Gateway donor vector pDONR201. The ORF sequence in the entry clone is flanked with attL1 sequence (100 nt) - spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) - spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the translational initiation codon (ATG) and is also flanked with a spacer nucleotide (G) - attL2 sequence (99 nt) downstream of the ORF. This is an N-type clone which has an intrinsic stop codon at the end of ORF. DNA sequences of the entire ORF and flanking regions were validated by sequencing. Clone structure of the ORF and flanking sequences is as follows: 5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3' G acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaacgaa caggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL sequences are described in lower cases). Clone information: http://www.HGPD.jp/ This clone was produced in the "Functional Analysis of Protein and Research Application" project supported by the New Energy and Industrial Technology Development Organization (NEDO), Japan FEATURES Location/Qualifiers source 1..597 /clone="FLJ08184AAAN" /db_xref="H-InvDB:HIT000487530" /db_xref="taxon:9606" /mol_type="mRNA" /note="Vector: pDONR201" /organism="Homo sapiens" CDS 1..597 /codon_start=1 /gene="YKT6" /product="YKT6 v-SNARE protein" /protein_id="BAG70131.1" /transl_table=1 /translation="MKLYSLSVLYKGEAKVVLLKAAYDVSSLSFFQRSSVQEFMTFTS QLIVERSSKGTRASVKEQDYLCHVYVRNDSLAGVVIADNEYPSRVAFTLLEKVLDEFS KQVDRIDWPVGSPATIHYPALDGHLSRYQNPREADPMTKVQAELDETKIILHNTMESL LERGEKLDDLVSKSEVLGTQSKAFYKTARKQNSCCAIM" BASE COUNT 167 a 153 c 143 g 134 t ORIGIN 1 atgaagctgt acagcctcag cgtcctctac aaaggcgagg ccaaggtggt gctgctcaaa 61 gccgcatacg atgtgtcttc cctcagcttt ttccagagat ccagcgttca ggaattcatg 121 accttcacga gtcaactgat tgtggagcgc tcatcgaaag gcactagagc ttctgtcaaa 181 gaacaagact atctgtgcca cgtctacgtc cggaatgata gtcttgcagg tgtggtcatt 241 gctgacaatg aatacccatc ccgggtggcc tttaccttgc tggagaaggt actagatgaa 301 ttctccaagc aagtcgacag gatagactgg ccagtaggat cccctgctac aatccattac 361 ccagccctgg atggtcacct cagtagatac cagaacccac gagaagctga tcccatgact 421 aaagtgcagg ccgaactaga tgagaccaaa atcattctgc acaacaccat ggagtctctg 481 ttagagcgag gtgagaagct agatgacttg gtgtccaaat ccgaggtgct gggaacacag 541 tctaaagcct tctataaaac tgcccggaaa caaaactcat gctgtgccat catgtaa //