LOCUS       AB451313                2052 bp    mRNA    linear   HUM 02-SEP-2008
DEFINITION  Homo sapiens PRKCH mRNA for protein kinase C eta type, complete
            cds, clone: FLJ08174AAAN.
ACCESSION   AB451313
VERSION     AB451313.1
KEYWORDS    Gateway cloning system.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Kawamura,Y., Nomura,N. and Goshima,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases.
            Contact:Naoki Goshima
            Biomedicinal Information Research Center (BIRC), National
            Institute of Advanced Industrial Science and Technology (AIST);
            2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan
REFERENCE   2
  AUTHORS   Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R.,
            Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T.,
            Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C.,
            Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M.,
            Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K.,
            Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A.,
            Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y.,
            Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T.,
            Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H.,
            Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T.,
            Imai,J.-I., Watanabe,S. and Nomura,N.
  TITLE     Human Protein Factory: an infrastructure to convert the human
            transcriptome into the in vitro-expressed human proteome of
            versatile utility
  JOURNAL   Unpublished (2008)
COMMENT     This human Gateway entry clone was constructed by recombining an
            attB-attached open reading frame (ORF) fragment with attP
            sequences of the Gateway donor vector pDONR201. The ORF sequence
            in the entry clone is flanked with attL1 sequence (100 nt) -
            spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) -
            spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the
            translational initiation codon (ATG) and is also flanked with a
            spacer nucleotide (G) - attL2 sequence (99 nt) downstream of the
            ORF. This is an N-type clone which has an intrinsic stop codon at
            the end of ORF. DNA sequences of the entire ORF and flanking
            regions were validated by sequencing. Clone structure of the ORF
            and flanking sequences is as follows:
            5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct
            tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3'
            G acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaacgaa
            caggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL
            sequences are described in lower cases).
            Clone information: http://www.HGPD.jp/
            This clone was produced in the "Functional Analysis of Protein and
            Research Application" project supported by the New Energy and
            Industrial Technology Development Organization (NEDO), Japan
FEATURES             Location/Qualifiers
     source          1..2052
                     /clone="FLJ08174AAAN"
                     /db_xref="H-InvDB:HIT000487526"
                     /db_xref="taxon:9606"
                     /mol_type="mRNA"
                     /note="Vector: pDONR201"
                     /organism="Homo sapiens"
     CDS             1..2052
                     /codon_start=1
                     /gene="PRKCH"
                     /product="protein kinase C eta type"
                     /protein_id="BAG70127.1"
                     /transl_table=1
                     /translation="MSSGTMKFNGYLRVRIGEAVGLQPTRWSPRHSLFKKGHQLLDPY
                     LTVSVDQVRVGQTSTKQKTNKPTYNEEFCANVTDGGHLELAVFHETPLGYDHFVANCT
                     LQFQELLRTTGASDTFEGWVDLEPEGKVFVVITLTGSFTEATLQRDRIFKHFTRKRQR
                     AMRRRVHQINGHKFMATYLRQPTYCSHCREFIWGVFGKQGYQCQVCTCVVHKRCHHLI
                     VTACTCQNNINKVDSKIAEQRFGINIPHKFSIHNYKVPTFCDHCGSLLWGIMRQGLQC
                     KICKMNVHIRCQANVAPNCGVNAVELAKTLAGMGLQPGNISPTSKLVSRSTLRRQGKE
                     SSKEGNGIGVNSSNRLGIDNFEFIRVLGKGSFGKVMLARVKETGDLYAVKVLKKDVIL
                     QDDDVECTMTEKRILSLARNHPFLTQLFCCFQTPDRLFFVMEFVNGGDLMFHIQKSRR
                     FDEARARFYAAEIISALMFLHDKGIIYRDLKLDNVLLDHEGHCKLADFGMCKEGICNG
                     VTTATFCGTPDYIAPEILQEMLYGPAVDWWAMGVLLYEMLCGHAPFEAENEDDLFEAI
                     LNDEVVYPTWLHEDATGILKSFMTKNPTMRLGSLTQGGEHAILRHPFFKEIDWAQLNH
                     RQIEPPFRPRIKSREDVSNFDPDFIKEEPVLTPIDEGHLPMINQDEFRNFSYVSPELQ
                     P"
BASE COUNT          532 a          512 c          538 g          470 t
ORIGIN      
        1 atgtcgtctg gcaccatgaa gttcaatggc tatttgaggg tccgcatcgg tgaggcagtg
       61 gggctgcagc ccacccgctg gtccccgcgc cactcgctct tcaagaaggg ccaccagctg
      121 ctggacccct atctgacggt gagcgtggac caggtgcgcg tgggccagac cagcaccaag
      181 cagaagacca acaaacccac gtacaacgag gagttttgcg ctaacgtcac cgacggcggc
      241 cacctcgagt tggccgtctt ccacgagacg cccctgggct acgaccactt cgtggccaac
      301 tgcaccctgc agttccagga gctgctgcgc acgaccggcg cctcggacac cttcgagggt
      361 tgggtggatc tcgagccaga ggggaaagta tttgtggtaa taacccttac cgggagtttc
      421 actgaagcta ctctccagag agaccggatc ttcaaacatt ttaccaggaa gcgccaaagg
      481 gctatgcgaa ggcgagtcca ccagatcaat ggacacaagt tcatggccac gtatctgagg
      541 cagcccacct actgctctca ctgcagggag tttatctggg gagtgtttgg gaaacagggt
      601 tatcagtgcc aagtgtgcac ctgtgtcgtc cataaacgct gccatcatct aattgttaca
      661 gcctgtactt gccaaaacaa tattaacaaa gtggattcaa agattgcaga acagaggttc
      721 gggatcaaca tcccacacaa gttcagcatc cacaactaca aagtgccaac attctgcgat
      781 cactgtggct cactgctctg gggaataatg cgacaaggac ttcagtgtaa aatatgtaaa
      841 atgaatgtgc atattcgatg tcaagcgaac gtggccccta actgtggggt aaatgcggtg
      901 gaacttgcca agaccctggc agggatgggt ctccaacccg gaaatatttc tccaacctcg
      961 aaactcgttt ccagatcgac cctaagacga cagggaaagg agagcagcaa agaaggaaat
     1021 gggattgggg ttaattcttc caaccgactt ggtatcgaca actttgagtt catccgagtg
     1081 ttggggaagg ggagttttgg gaaggtgatg cttgcaagag taaaagaaac aggagacctc
     1141 tatgctgtga aggtgctgaa gaaggacgtg attctgcagg atgatgatgt ggaatgcacc
     1201 atgaccgaga aaaggatcct gtctctggcc cgcaatcacc ccttcctcac tcagttgttc
     1261 tgctgctttc agacccccga tcgtctgttt tttgtgatgg agtttgtgaa tgggggtgac
     1321 ttgatgttcc acattcagaa gtctcgtcgt tttgatgaag cacgagctcg cttctatgct
     1381 gcagaaatca tttcggccct catgttcctc catgataaag gaatcatcta tagagatctg
     1441 aaactggaca atgtcctgtt ggaccacgag ggtcactgta aactggcaga cttcggaatg
     1501 tgcaaggagg ggatttgcaa tggtgtcacc acggccacat tctgtggcac gccagactat
     1561 atcgctccag agatcctcca ggaaatgctg tacgggcctg cagtagactg gtgggcaatg
     1621 ggcgtgttgc tctatgagat gctctgtggc cacgcgcctt ttgaggcaga gaatgaagat
     1681 gacctctttg aggccatact gaatgatgag gtggtctacc ctacctggct ccatgaagat
     1741 gccacaggga tcctaaaatc tttcatgacc aagaacccca ccatgcgctt gggcagcctg
     1801 actcagggag gcgagcacgc catcttgaga catccttttt ttaaggaaat cgactgggcc
     1861 cagctgaacc atcgccaaat agaaccgcct ttcagaccca gaatcaaatc ccgagaagat
     1921 gtcagtaatt ttgaccctga cttcataaag gaagagccag ttttaactcc aattgatgag
     1981 ggacatcttc caatgattaa ccaggatgag tttagaaact tttcctatgt gtctccagaa
     2041 ttgcaaccat aa
//