LOCUS AB451313 2052 bp mRNA linear HUM 02-SEP-2008 DEFINITION Homo sapiens PRKCH mRNA for protein kinase C eta type, complete cds, clone: FLJ08174AAAN. ACCESSION AB451313 VERSION AB451313.1 KEYWORDS Gateway cloning system. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 2052) AUTHORS Kawamura,Y., Nomura,N. and Goshima,N. TITLE Direct Submission JOURNAL Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases. Contact:Naoki Goshima Biomedicinal Information Research Center (BIRC), National Institute of Advanced Industrial Science and Technology (AIST); 2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan REFERENCE 2 AUTHORS Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R., Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T., Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C., Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M., Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K., Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A., Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y., Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T., Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H., Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T., Imai,J.-I., Watanabe,S. and Nomura,N. TITLE Human Protein Factory: an infrastructure to convert the human transcriptome into the in vitro-expressed human proteome of versatile utility JOURNAL Unpublished (2008) COMMENT This human Gateway entry clone was constructed by recombining an attB-attached open reading frame (ORF) fragment with attP sequences of the Gateway donor vector pDONR201. The ORF sequence in the entry clone is flanked with attL1 sequence (100 nt) - spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) - spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the translational initiation codon (ATG) and is also flanked with a spacer nucleotide (G) - attL2 sequence (99 nt) downstream of the ORF. This is an N-type clone which has an intrinsic stop codon at the end of ORF. DNA sequences of the entire ORF and flanking regions were validated by sequencing. Clone structure of the ORF and flanking sequences is as follows: 5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3' G acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaacgaa caggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL sequences are described in lower cases). Clone information: http://www.HGPD.jp/ This clone was produced in the "Functional Analysis of Protein and Research Application" project supported by the New Energy and Industrial Technology Development Organization (NEDO), Japan FEATURES Location/Qualifiers source 1..2052 /clone="FLJ08174AAAN" /db_xref="H-InvDB:HIT000487526" /db_xref="taxon:9606" /mol_type="mRNA" /note="Vector: pDONR201" /organism="Homo sapiens" CDS 1..2052 /codon_start=1 /gene="PRKCH" /product="protein kinase C eta type" /protein_id="BAG70127.1" /transl_table=1 /translation="MSSGTMKFNGYLRVRIGEAVGLQPTRWSPRHSLFKKGHQLLDPY LTVSVDQVRVGQTSTKQKTNKPTYNEEFCANVTDGGHLELAVFHETPLGYDHFVANCT LQFQELLRTTGASDTFEGWVDLEPEGKVFVVITLTGSFTEATLQRDRIFKHFTRKRQR AMRRRVHQINGHKFMATYLRQPTYCSHCREFIWGVFGKQGYQCQVCTCVVHKRCHHLI VTACTCQNNINKVDSKIAEQRFGINIPHKFSIHNYKVPTFCDHCGSLLWGIMRQGLQC KICKMNVHIRCQANVAPNCGVNAVELAKTLAGMGLQPGNISPTSKLVSRSTLRRQGKE SSKEGNGIGVNSSNRLGIDNFEFIRVLGKGSFGKVMLARVKETGDLYAVKVLKKDVIL QDDDVECTMTEKRILSLARNHPFLTQLFCCFQTPDRLFFVMEFVNGGDLMFHIQKSRR FDEARARFYAAEIISALMFLHDKGIIYRDLKLDNVLLDHEGHCKLADFGMCKEGICNG VTTATFCGTPDYIAPEILQEMLYGPAVDWWAMGVLLYEMLCGHAPFEAENEDDLFEAI LNDEVVYPTWLHEDATGILKSFMTKNPTMRLGSLTQGGEHAILRHPFFKEIDWAQLNH RQIEPPFRPRIKSREDVSNFDPDFIKEEPVLTPIDEGHLPMINQDEFRNFSYVSPELQ P" BASE COUNT 532 a 512 c 538 g 470 t ORIGIN 1 atgtcgtctg gcaccatgaa gttcaatggc tatttgaggg tccgcatcgg tgaggcagtg 61 gggctgcagc ccacccgctg gtccccgcgc cactcgctct tcaagaaggg ccaccagctg 121 ctggacccct atctgacggt gagcgtggac caggtgcgcg tgggccagac cagcaccaag 181 cagaagacca acaaacccac gtacaacgag gagttttgcg ctaacgtcac cgacggcggc 241 cacctcgagt tggccgtctt ccacgagacg cccctgggct acgaccactt cgtggccaac 301 tgcaccctgc agttccagga gctgctgcgc acgaccggcg cctcggacac cttcgagggt 361 tgggtggatc tcgagccaga ggggaaagta tttgtggtaa taacccttac cgggagtttc 421 actgaagcta ctctccagag agaccggatc ttcaaacatt ttaccaggaa gcgccaaagg 481 gctatgcgaa ggcgagtcca ccagatcaat ggacacaagt tcatggccac gtatctgagg 541 cagcccacct actgctctca ctgcagggag tttatctggg gagtgtttgg gaaacagggt 601 tatcagtgcc aagtgtgcac ctgtgtcgtc cataaacgct gccatcatct aattgttaca 661 gcctgtactt gccaaaacaa tattaacaaa gtggattcaa agattgcaga acagaggttc 721 gggatcaaca tcccacacaa gttcagcatc cacaactaca aagtgccaac attctgcgat 781 cactgtggct cactgctctg gggaataatg cgacaaggac ttcagtgtaa aatatgtaaa 841 atgaatgtgc atattcgatg tcaagcgaac gtggccccta actgtggggt aaatgcggtg 901 gaacttgcca agaccctggc agggatgggt ctccaacccg gaaatatttc tccaacctcg 961 aaactcgttt ccagatcgac cctaagacga cagggaaagg agagcagcaa agaaggaaat 1021 gggattgggg ttaattcttc caaccgactt ggtatcgaca actttgagtt catccgagtg 1081 ttggggaagg ggagttttgg gaaggtgatg cttgcaagag taaaagaaac aggagacctc 1141 tatgctgtga aggtgctgaa gaaggacgtg attctgcagg atgatgatgt ggaatgcacc 1201 atgaccgaga aaaggatcct gtctctggcc cgcaatcacc ccttcctcac tcagttgttc 1261 tgctgctttc agacccccga tcgtctgttt tttgtgatgg agtttgtgaa tgggggtgac 1321 ttgatgttcc acattcagaa gtctcgtcgt tttgatgaag cacgagctcg cttctatgct 1381 gcagaaatca tttcggccct catgttcctc catgataaag gaatcatcta tagagatctg 1441 aaactggaca atgtcctgtt ggaccacgag ggtcactgta aactggcaga cttcggaatg 1501 tgcaaggagg ggatttgcaa tggtgtcacc acggccacat tctgtggcac gccagactat 1561 atcgctccag agatcctcca ggaaatgctg tacgggcctg cagtagactg gtgggcaatg 1621 ggcgtgttgc tctatgagat gctctgtggc cacgcgcctt ttgaggcaga gaatgaagat 1681 gacctctttg aggccatact gaatgatgag gtggtctacc ctacctggct ccatgaagat 1741 gccacaggga tcctaaaatc tttcatgacc aagaacccca ccatgcgctt gggcagcctg 1801 actcagggag gcgagcacgc catcttgaga catccttttt ttaaggaaat cgactgggcc 1861 cagctgaacc atcgccaaat agaaccgcct ttcagaccca gaatcaaatc ccgagaagat 1921 gtcagtaatt ttgaccctga cttcataaag gaagagccag ttttaactcc aattgatgag 1981 ggacatcttc caatgattaa ccaggatgag tttagaaact tttcctatgt gtctccagaa 2041 ttgcaaccat aa //