LOCUS AB451311 1323 bp mRNA linear HUM 02-SEP-2008
DEFINITION Homo sapiens PSMC1 mRNA for proteasome 26S ATPase subunit 1,
complete cds, clone: FLJ08170AAAN.
ACCESSION AB451311
VERSION AB451311.1
KEYWORDS Gateway cloning system.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1323)
AUTHORS Kawamura,Y., Nomura,N. and Goshima,N.
TITLE Direct Submission
JOURNAL Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Naoki Goshima
Biomedicinal Information Research Center (BIRC), National
Institute of Advanced Industrial Science and Technology (AIST);
2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan
REFERENCE 2
AUTHORS Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R.,
Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T.,
Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C.,
Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M.,
Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K.,
Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A.,
Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y.,
Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T.,
Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H.,
Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T.,
Imai,J.-I., Watanabe,S. and Nomura,N.
TITLE Human Protein Factory: an infrastructure to convert the human
transcriptome into the in vitro-expressed human proteome of
versatile utility
JOURNAL Unpublished (2008)
COMMENT This human Gateway entry clone was constructed by recombining an
attB-attached open reading frame (ORF) fragment with attP
sequences of the Gateway donor vector pDONR201. The ORF sequence
in the entry clone is flanked with attL1 sequence (100 nt) -
spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) -
spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the
translational initiation codon (ATG) and is also flanked with a
spacer nucleotide (G) - attL2 sequence (99 nt) downstream of the
ORF. This is an N-type clone which has an intrinsic stop codon at
the end of ORF. DNA sequences of the entire ORF and flanking
regions were validated by sequencing. Clone structure of the ORF
and flanking sequences is as follows:
5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct
tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3'
G acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaacgaa
caggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL
sequences are described in lower cases).
Clone information: http://www.HGPD.jp/
This clone was produced in the "Functional Analysis of Protein and
Research Application" project supported by the New Energy and
Industrial Technology Development Organization (NEDO), Japan
FEATURES Location/Qualifiers
source 1..1323
/clone="FLJ08170AAAN"
/db_xref="H-InvDB:HIT000487524"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="Vector: pDONR201"
/organism="Homo sapiens"
CDS 1..1323
/codon_start=1
/gene="PSMC1"
/product="proteasome 26S ATPase subunit 1"
/protein_id="BAG70125.1"
/transl_table=1
/translation="MGQSQSGGHGPGGGKKDDKDKKKKYEPPVPTRVGKKKKKTKGPD
AASKLPLVTPHTQCRLKLLKLERIKDYLLMEEEFIRNQEQMKPLEEKQEEERSKVDDL
RGTPMSVGTLEEIIDDNHAIVSTSVGSEHYVSILSFVDKDLLEPGCSVLLNHKVHAVI
GVLMDDTDPLVTVMKVEKAPQETYADIGGLDNQIQEIKESVELPLTHPEYYEEMGIKP
PKGVILYGPPGTGKTLLAKAVANQTSATFLRVVGSELIQKYLGDGPKLVRELFRVAEE
HAPSIVFIDEIDAIGTKRYDSNSGGEREIQRTMLELLNQLDGFDSRGDVKVIMATNRI
ETLDPALIRPGRIDRKIEFPLPDEKTKKRIFQIHTSRMTLADDVTLDDLIMAKDDLSG
ADIKAICTEAGLMALRERRMKVTNEDFKKSKENVLYKKQEGTPEGLYL"
BASE COUNT 426 a 264 c 340 g 293 t
ORIGIN
1 atgggtcaaa gtcagagtgg tggtcatggt cctggaggtg gcaagaagga tgacaaggac
61 aagaaaaaga aatatgaacc tcctgtacca actagagtgg ggaaaaagaa gaagaaaaca
121 aagggaccag atgctgccag caaactgcca ctggtgacac ctcacactca gtgccggtta
181 aaattactga agttagagag aattaaagac tatcttctca tggaggaaga attcattaga
241 aatcaggaac aaatgaaacc attagaagaa aagcaagagg aggaaagatc aaaagtggat
301 gatctgaggg ggaccccgat gtcagtagga accttggaag agatcattga tgacaatcat
361 gccatcgtgt ctacatctgt gggctcagaa cactacgtca gcattctttc atttgtagac
421 aaggatctgc tggaacctgg ctgctcggtc ctgctcaacc acaaggtgca tgccgtgata
481 ggggtgctga tggatgacac ggatcccctg gtcacagtga tgaaggtaga aaaggccccc
541 caggagacct atgcagatat tggggggttg gacaaccaaa ttcaggaaat taaggaatct
601 gtggagcttc ctctcaccca tcctgaatat tatgaagaga tgggtataaa gcctcctaag
661 ggggtcattc tctatggtcc acctggcaca ggtaaaacct tgttagccaa agcagtagca
721 aaccaaacct cagccacttt cttgagagtg gttggctctg aacttattca gaagtaccta
781 ggtgatgggc ccaaactcgt acgggaattg ttccgagttg ctgaagaaca tgcaccgtcc
841 atcgtgttta ttgatgaaat tgacgccatt gggacaaaaa gatatgactc caattctggt
901 ggtgagagag aaattcagcg aacaatgttg gaactgctga accagttgga tggatttgat
961 tctaggggag atgtgaaagt tatcatggcc acaaaccgaa tagaaacttt ggatccagca
1021 cttatcagac caggccgcat tgacaggaag attgagttcc ccctgcctga tgaaaagacg
1081 aagaagcgca tctttcagat tcacacaagc aggatgacgc tggctgatga tgtaaccctg
1141 gacgacctga tcatggctaa agatgacctc tctggtgctg acatcaaggc aatctgtaca
1201 gaagctggtc tgatggcctt aagagaacgt agaatgaaag taacaaatga agacttcaaa
1261 aaatctaaag aaaatgttct ttataagaaa caggaaggca cccctgaggg gctgtatctc
1321 taa
//