LOCUS AB451295 1338 bp mRNA linear HUM 02-SEP-2008 DEFINITION Homo sapiens GDI2 mRNA for GDP dissociation inhibitor 2 isoform 1, complete cds, clone: FLJ08144AAAN. ACCESSION AB451295 VERSION AB451295.1 KEYWORDS Gateway cloning system. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1338) AUTHORS Kawamura,Y., Nomura,N. and Goshima,N. TITLE Direct Submission JOURNAL Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases. Contact:Naoki Goshima Biomedicinal Information Research Center (BIRC), National Institute of Advanced Industrial Science and Technology (AIST); 2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan REFERENCE 2 AUTHORS Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R., Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T., Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C., Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M., Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K., Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A., Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y., Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T., Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H., Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T., Imai,J.-I., Watanabe,S. and Nomura,N. TITLE Human Protein Factory: an infrastructure to convert the human transcriptome into the in vitro-expressed human proteome of versatile utility JOURNAL Unpublished (2008) COMMENT This human Gateway entry clone was constructed by recombining an attB-attached open reading frame (ORF) fragment with attP sequences of the Gateway donor vector pDONR201. The ORF sequence in the entry clone is flanked with attL1 sequence (100 nt) - spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) - spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the translational initiation codon (ATG) and is also flanked with a spacer nucleotide (G) - attL2 sequence (99 nt) downstream of the ORF. This is an N-type clone which has an intrinsic stop codon at the end of ORF. DNA sequences of the entire ORF and flanking regions were validated by sequencing. Clone structure of the ORF and flanking sequences is as follows: 5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3' G acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaacgaa caggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL sequences are described in lower cases). Clone information: http://www.HGPD.jp/ This clone was produced in the "Functional Analysis of Protein and Research Application" project supported by the New Energy and Industrial Technology Development Organization (NEDO), Japan FEATURES Location/Qualifiers source 1..1338 /clone="FLJ08144AAAN" /db_xref="H-InvDB:HIT000487508" /db_xref="taxon:9606" /mol_type="mRNA" /note="Vector: pDONR201" /organism="Homo sapiens" CDS 1..1338 /codon_start=1 /gene="GDI2" /product="GDP dissociation inhibitor 2 isoform 1" /protein_id="BAG70109.1" /transl_table=1 /translation="MNEEYDVIVLGTGLTECILSGIMSVNGKKVLHMDRNPYYGGESA SITPLEDLYKRFKIPGSPPESMGRGRDWNVDLIPKFLMANGQLVKMLLYTEVTRYLDF KVTEGSFVYKGGKIYKVPSTEAEALASSLMGLFEKRRFRKFLVYVANFDEKDPRTFEG IDPKKTTMRDVYKKFDLGQDVIDFTGHALALYRTDDYLDQPCYETINRIKLYSESLAR YGKSPYLYPLYGLGELPQGFARLSAIYGGTYMLNKPIEEIIVQNGKVIGVKSEGEIAR CKQLICDPSYVKDRVEKVGQVIRVICILSHPIKNTNDANSCQIIIPQNQVNRKSDIYV CMISFAHNVAAQGKYIAIVSTTVETKEPEKEIRPALELLEPIEQKFVSISDLLVPKDL GTESQIFISRTYDATTHFETTCDDIKNIYKRMTGSEFDFEEMKRKKNDIYGED" BASE COUNT 436 a 256 c 304 g 342 t ORIGIN 1 atgaatgagg agtacgacgt gatcgtgctg ggcaccggcc tgacggaatg tatcctgtca 61 ggtataatgt cagtgaatgg caagaaagtt cttcatatgg atcgaaaccc ttactacgga 121 ggagagagtg catctataac accattggaa gatttataca aaagatttaa aataccagga 181 tcaccacccg agtcaatggg gagaggaaga gactggaatg ttgacttgat tcccaagttc 241 cttatggcta atggtcagct ggttaagatg ctgctttata cagaggtaac tcgctatctg 301 gattttaaag tgactgaagg gagctttgtc tataagggtg gaaaaatcta caaggttcct 361 tccactgaag cagaagccct ggcatctagc ctaatgggat tgtttgaaaa acgtcgcttc 421 aggaaattcc tagtgtatgt tgccaacttc gatgaaaaag atccaagaac ttttgaaggc 481 attgatccta agaagaccac aatgcgagat gtgtataaga aatttgattt gggtcaagac 541 gttatagatt ttactggtca tgctcttgca ctttacagaa ctgatgatta cttagatcaa 601 ccgtgttatg aaaccattaa tagaattaaa ctttacagtg aatctttggc aagatatggc 661 aaaagcccat acctttatcc actctatggc cttggagaac tgccccaagg atttgcaagg 721 ctaagtgcta tttatggagg tacctatatg ctgaataaac ccattgaaga aatcattgta 781 cagaatggaa aagtaattgg tgtaaaatct gaaggagaaa ttgctcgctg taagcagctc 841 atctgtgacc ccagctacgt aaaagatcgg gtagaaaaag tgggccaggt gatcagagtt 901 atttgcatcc tcagccaccc catcaagaac accaatgatg ccaactcctg ccagatcatt 961 attccacaga accaagtcaa tcgaaagtca gatatctacg tctgcatgat ctcctttgcg 1021 cacaatgtag cagcacaagg gaagtacatt gctatagtta gtacaactgt ggaaaccaag 1081 gagcctgaga aggaaatcag accagctttg gagctcttgg aaccaattga acagaaattt 1141 gttagcatca gtgacctcct ggtaccaaaa gacttgggaa cagaaagcca gatctttatt 1201 tcccgcacat atgatgccac cactcatttt gagacaacgt gtgatgacat taaaaacatc 1261 tataagagga tgacaggatc agagtttgac tttgaggaaa tgaagcgcaa gaagaatgac 1321 atctatgggg aagactaa //