LOCUS AB451293 1614 bp mRNA linear HUM 02-SEP-2008
DEFINITION Homo sapiens FYN mRNA for protein-tyrosine kinase fyn isoform a,
complete cds, clone: FLJ08138AAAN.
ACCESSION AB451293
VERSION AB451293.1
KEYWORDS Gateway cloning system.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1614)
AUTHORS Kawamura,Y., Nomura,N. and Goshima,N.
TITLE Direct Submission
JOURNAL Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Naoki Goshima
Biomedicinal Information Research Center (BIRC), National
Institute of Advanced Industrial Science and Technology (AIST);
2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan
REFERENCE 2
AUTHORS Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R.,
Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T.,
Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C.,
Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M.,
Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K.,
Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A.,
Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y.,
Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T.,
Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H.,
Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T.,
Imai,J.-I., Watanabe,S. and Nomura,N.
TITLE Human Protein Factory: an infrastructure to convert the human
transcriptome into the in vitro-expressed human proteome of
versatile utility
JOURNAL Unpublished (2008)
COMMENT This human Gateway entry clone was constructed by recombining an
attB-attached open reading frame (ORF) fragment with attP
sequences of the Gateway donor vector pDONR201. The ORF sequence
in the entry clone is flanked with attL1 sequence (100 nt) -
spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) -
spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the
translational initiation codon (ATG) and is also flanked with a
spacer nucleotide (G) - attL2 sequence (99 nt) downstream of the
ORF. This is an N-type clone which has an intrinsic stop codon at
the end of ORF. DNA sequences of the entire ORF and flanking
regions were validated by sequencing. Clone structure of the ORF
and flanking sequences is as follows:
5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct
tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3'
G acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaacgaa
caggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL
sequences are described in lower cases).
Clone information: http://www.HGPD.jp/
This clone was produced in the "Functional Analysis of Protein and
Research Application" project supported by the New Energy and
Industrial Technology Development Organization (NEDO), Japan
FEATURES Location/Qualifiers
source 1..1614
/clone="FLJ08138AAAN"
/db_xref="H-InvDB:HIT000487506"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="Vector: pDONR201"
/organism="Homo sapiens"
CDS 1..1614
/codon_start=1
/gene="FYN"
/product="protein-tyrosine kinase fyn isoform a"
/protein_id="BAG70107.1"
/transl_table=1
/translation="MGCVQCKDKEATKLTEERDGSLNQSSGYRYGTDPTPQHYPSFGV
TSIPNYNNFHAAGGQGLTVFGGVNSSSHTGTLRTRGGTGVTLFVALYDYEARTEDDLS
FHKGEKFQILNSSEGDWWEARSLTTGETGYIPSNYVAPVDSIQAEEWYFGKLGRKDAE
RQLLSFGNPRGTFLIRESETTKGAYSLSIRDWDDMKGDHVKHYKIRKLDNGGYYITTR
AQFETLQQLVQHYSERAAGLCCRLVVPCHKGMPRLTDLSVKTKDVWEIPRESLQLIKR
LGNGQFGEVWMGTWNGNTKVAIKTLKPGTMSPESFLEEAQIMKKLKHDKLVQLYAVVS
EEPIYIVTEYMNKGSLLDFLKDGEGRALKLPNLVDMAAQVAAGMAYIERMNYIHRDLR
SANILVGNGLICKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVW
SFGILLTELVTKGRVPYPGMNNREVLEQVERGYRMPCPQDCPISLHELMIHCWKKDPE
ERPTFEYLQSFLEDYFTATEPQYQPGENL"
BASE COUNT 469 a 386 c 417 g 342 t
ORIGIN
1 atgggctgtg tgcaatgtaa ggataaagaa gcaacaaaac tgacggagga gagggacggc
61 agcctgaacc agagctctgg gtaccgctat ggcacagacc ccacccctca gcactacccc
121 agcttcggtg tgacctccat ccccaactac aacaacttcc acgcagccgg gggccaagga
181 ctcaccgtct ttggaggtgt gaactcttcg tctcatacgg ggaccttgcg tacgagagga
241 ggaacaggag tgacactctt tgtggccctt tatgactatg aagcacggac agaagatgac
301 ctgagttttc acaaaggaga aaaatttcaa atattgaaca gctcggaagg agattggtgg
361 gaagcccgct ccttgacaac tggagagaca ggttacattc ccagcaatta tgtggctcca
421 gttgactcta tccaggcaga agagtggtac tttggaaaac ttggccgaaa agatgctgag
481 cgacagctat tgtcctttgg aaacccaaga ggtacctttc ttatccgcga gagtgaaacc
541 accaaaggtg cctattcact ttctatccgt gattgggatg atatgaaagg agaccatgtc
601 aaacattata aaattcgcaa acttgacaat ggtggatact acattaccac ccgggcccag
661 tttgaaacac ttcagcagct tgtacaacat tactcagaga gagctgcagg tctctgctgc
721 cgcctagtag ttccctgtca caaagggatg ccaaggctta ccgatctgtc tgtcaaaacc
781 aaagatgtct gggaaatccc tcgagaatcc ctgcagttga tcaagagact gggaaatggg
841 cagtttgggg aagtatggat gggtacctgg aatggaaaca caaaagtagc cataaagact
901 cttaaaccag gcacaatgtc ccccgaatca ttccttgagg aagcgcagat catgaagaag
961 ctgaagcacg acaagctggt ccagctctat gcagtggtgt ctgaggagcc catctacatc
1021 gtcaccgagt atatgaacaa aggaagttta ctggatttct taaaagatgg agaaggaaga
1081 gctctgaaat taccaaatct tgtggacatg gcagcacagg tggctgcagg aatggcttac
1141 atcgagcgca tgaattatat ccatagagat ctgcgatcag caaacattct agtggggaat
1201 ggactcatat gcaagattgc tgacttcgga ttggcccgat tgatagaaga caatgagtac
1261 acagcaagac aaggtgcaaa gttccccatc aagtggacgg cccccgaggc agccctgtac
1321 gggaggttca caatcaagtc tgacgtgtgg tcttttggaa tcttactcac agagctggtc
1381 accaaaggaa gagtgccata cccaggcatg aacaaccggg aggtgctgga gcaggtggag
1441 cgaggctaca ggatgccctg cccgcaggac tgccccatct ctctgcatga gctcatgatc
1501 cactgctgga aaaaggaccc tgaagaacgc cccacttttg agtacttgca gagcttcctg
1561 gaagactact ttaccgcgac agagccccag taccaacctg gtgaaaacct gtaa
//