LOCUS AB451289 1119 bp mRNA linear HUM 02-SEP-2008 DEFINITION Homo sapiens CDK9 mRNA for cyclin-dependent kinase 9, complete cds, clone: FLJ08130AAAN. ACCESSION AB451289 VERSION AB451289.1 KEYWORDS Gateway cloning system. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1119) AUTHORS Kawamura,Y., Nomura,N. and Goshima,N. TITLE Direct Submission JOURNAL Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases. Contact:Naoki Goshima Biomedicinal Information Research Center (BIRC), National Institute of Advanced Industrial Science and Technology (AIST); 2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan REFERENCE 2 AUTHORS Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R., Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T., Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C., Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M., Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K., Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A., Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y., Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T., Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H., Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T., Imai,J.-I., Watanabe,S. and Nomura,N. TITLE Human Protein Factory: an infrastructure to convert the human transcriptome into the in vitro-expressed human proteome of versatile utility JOURNAL Unpublished (2008) COMMENT This human Gateway entry clone was constructed by recombining an attB-attached open reading frame (ORF) fragment with attP sequences of the Gateway donor vector pDONR201. The ORF sequence in the entry clone is flanked with attL1 sequence (100 nt) - spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) - spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the translational initiation codon (ATG) and is also flanked with a spacer nucleotide (G) - attL2 sequence (99 nt) downstream of the ORF. This is an N-type clone which has an intrinsic stop codon at the end of ORF. DNA sequences of the entire ORF and flanking regions were validated by sequencing. Clone structure of the ORF and flanking sequences is as follows: 5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3' G acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaacgaa caggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL sequences are described in lower cases). Clone information: http://www.HGPD.jp/ This clone was produced in the "Functional Analysis of Protein and Research Application" project supported by the New Energy and Industrial Technology Development Organization (NEDO), Japan FEATURES Location/Qualifiers source 1..1119 /clone="FLJ08130AAAN" /db_xref="H-InvDB:HIT000487502" /db_xref="taxon:9606" /mol_type="mRNA" /note="Vector: pDONR201" /organism="Homo sapiens" CDS 1..1119 /codon_start=1 /gene="CDK9" /product="cyclin-dependent kinase 9" /protein_id="BAG70103.1" /transl_table=1 /translation="MAKQYDSVECPFCDEVSKYEKLAKIGQGTFGEVFKARHRKTGQK VALKKVLMENEKEGFPITALREIKILQLLKHENVVNLIEICRTKASPYNRCKGSIYLV FDFCEHDLAGLLSNVLVKFTLSEIKRVMQMLLNGLYYIHRNKILHRDMKAANVLITRD GVLKLADFGLARAFSLAKDSQPNRYTNRVVTLWYRPPELLLGERDYGPPIDLWGAGCI MAEMWTRSPIMQGNTEQHQLALISQLCGSITPEVWPNVDNYELYEKLELVKGQKRKVK DRLKAYVRDPYALDLIDKLLVLDPAQRIDSDDALNHDFFWSDPMPSDLKGMLSTHLTS MFEYLAPPRRKGSQITQQSTNQSRNPATTNQTEFERVF" BASE COUNT 262 a 327 c 315 g 215 t ORIGIN 1 atggcaaagc agtacgactc ggtggagtgc cctttttgtg atgaagtttc caaatacgag 61 aagctcgcca agatcggcca aggcaccttc ggggaggtgt tcaaggccag gcaccgcaag 121 accggccaga aggtggctct gaagaaggtg ctgatggaaa acgagaagga ggggttcccc 181 attacagcct tgcgggagat caagatcctt cagcttctaa aacacgagaa tgtggtcaac 241 ttgattgaga tttgtcgaac caaagcttcc ccctataacc gctgcaaggg tagtatatac 301 ctggtgttcg acttctgcga gcatgacctt gctgggctgt tgagcaatgt tttggtcaag 361 ttcacgctgt ctgagatcaa gagggtgatg cagatgctgc ttaacggcct ctactacatc 421 cacagaaaca agatcctgca tagggacatg aaggctgcta atgtgcttat cactcgtgat 481 ggggtcctga agctggcaga ctttgggctg gcccgggcct tcagcctggc caaggacagc 541 cagcccaacc gctacaccaa ccgtgtggtg acactctggt accggccccc ggagctgttg 601 ctcggggagc gggactacgg cccccccatt gacctgtggg gtgctgggtg catcatggca 661 gagatgtgga cccgcagccc catcatgcag ggcaacacgg agcagcacca actcgccctc 721 atcagtcagc tctgcggctc catcacccct gaggtgtggc caaacgtgga caactatgag 781 ctgtacgaaa agctggagct ggtcaagggc cagaagcgga aggtgaagga caggctgaag 841 gcctatgtgc gtgacccata cgcactggac ctcatcgaca agctgctggt gctggaccct 901 gcccagcgca tcgacagcga tgacgccctc aaccacgact tcttctggtc cgaccccatg 961 ccctccgacc tcaagggcat gctctccacc cacctgacgt ccatgttcga gtacttggca 1021 ccaccgcgcc ggaagggcag ccagatcacc cagcagtcca ccaaccagag tcgcaatccc 1081 gccaccacca accagacgga gtttgagcgc gtcttctaa //