LOCUS       AB451289                1119 bp    mRNA    linear   HUM 02-SEP-2008
DEFINITION  Homo sapiens CDK9 mRNA for cyclin-dependent kinase 9, complete
            cds, clone: FLJ08130AAAN.
ACCESSION   AB451289
VERSION     AB451289.1
KEYWORDS    Gateway cloning system.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Kawamura,Y., Nomura,N. and Goshima,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases.
            Contact:Naoki Goshima
            Biomedicinal Information Research Center (BIRC), National
            Institute of Advanced Industrial Science and Technology (AIST);
            2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan
REFERENCE   2
  AUTHORS   Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R.,
            Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T.,
            Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C.,
            Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M.,
            Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K.,
            Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A.,
            Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y.,
            Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T.,
            Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H.,
            Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T.,
            Imai,J.-I., Watanabe,S. and Nomura,N.
  TITLE     Human Protein Factory: an infrastructure to convert the human
            transcriptome into the in vitro-expressed human proteome of
            versatile utility
  JOURNAL   Unpublished (2008)
COMMENT     This human Gateway entry clone was constructed by recombining an
            attB-attached open reading frame (ORF) fragment with attP
            sequences of the Gateway donor vector pDONR201. The ORF sequence
            in the entry clone is flanked with attL1 sequence (100 nt) -
            spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) -
            spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the
            translational initiation codon (ATG) and is also flanked with a
            spacer nucleotide (G) - attL2 sequence (99 nt) downstream of the
            ORF. This is an N-type clone which has an intrinsic stop codon at
            the end of ORF. DNA sequences of the entire ORF and flanking
            regions were validated by sequencing. Clone structure of the ORF
            and flanking sequences is as follows:
            5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct
            tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3'
            G acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaacgaa
            caggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL
            sequences are described in lower cases).
            Clone information: http://www.HGPD.jp/
            This clone was produced in the "Functional Analysis of Protein and
            Research Application" project supported by the New Energy and
            Industrial Technology Development Organization (NEDO), Japan
FEATURES             Location/Qualifiers
     source          1..1119
                     /clone="FLJ08130AAAN"
                     /db_xref="H-InvDB:HIT000487502"
                     /db_xref="taxon:9606"
                     /mol_type="mRNA"
                     /note="Vector: pDONR201"
                     /organism="Homo sapiens"
     CDS             1..1119
                     /codon_start=1
                     /gene="CDK9"
                     /product="cyclin-dependent kinase 9"
                     /protein_id="BAG70103.1"
                     /transl_table=1
                     /translation="MAKQYDSVECPFCDEVSKYEKLAKIGQGTFGEVFKARHRKTGQK
                     VALKKVLMENEKEGFPITALREIKILQLLKHENVVNLIEICRTKASPYNRCKGSIYLV
                     FDFCEHDLAGLLSNVLVKFTLSEIKRVMQMLLNGLYYIHRNKILHRDMKAANVLITRD
                     GVLKLADFGLARAFSLAKDSQPNRYTNRVVTLWYRPPELLLGERDYGPPIDLWGAGCI
                     MAEMWTRSPIMQGNTEQHQLALISQLCGSITPEVWPNVDNYELYEKLELVKGQKRKVK
                     DRLKAYVRDPYALDLIDKLLVLDPAQRIDSDDALNHDFFWSDPMPSDLKGMLSTHLTS
                     MFEYLAPPRRKGSQITQQSTNQSRNPATTNQTEFERVF"
BASE COUNT          262 a          327 c          315 g          215 t
ORIGIN      
        1 atggcaaagc agtacgactc ggtggagtgc cctttttgtg atgaagtttc caaatacgag
       61 aagctcgcca agatcggcca aggcaccttc ggggaggtgt tcaaggccag gcaccgcaag
      121 accggccaga aggtggctct gaagaaggtg ctgatggaaa acgagaagga ggggttcccc
      181 attacagcct tgcgggagat caagatcctt cagcttctaa aacacgagaa tgtggtcaac
      241 ttgattgaga tttgtcgaac caaagcttcc ccctataacc gctgcaaggg tagtatatac
      301 ctggtgttcg acttctgcga gcatgacctt gctgggctgt tgagcaatgt tttggtcaag
      361 ttcacgctgt ctgagatcaa gagggtgatg cagatgctgc ttaacggcct ctactacatc
      421 cacagaaaca agatcctgca tagggacatg aaggctgcta atgtgcttat cactcgtgat
      481 ggggtcctga agctggcaga ctttgggctg gcccgggcct tcagcctggc caaggacagc
      541 cagcccaacc gctacaccaa ccgtgtggtg acactctggt accggccccc ggagctgttg
      601 ctcggggagc gggactacgg cccccccatt gacctgtggg gtgctgggtg catcatggca
      661 gagatgtgga cccgcagccc catcatgcag ggcaacacgg agcagcacca actcgccctc
      721 atcagtcagc tctgcggctc catcacccct gaggtgtggc caaacgtgga caactatgag
      781 ctgtacgaaa agctggagct ggtcaagggc cagaagcgga aggtgaagga caggctgaag
      841 gcctatgtgc gtgacccata cgcactggac ctcatcgaca agctgctggt gctggaccct
      901 gcccagcgca tcgacagcga tgacgccctc aaccacgact tcttctggtc cgaccccatg
      961 ccctccgacc tcaagggcat gctctccacc cacctgacgt ccatgttcga gtacttggca
     1021 ccaccgcgcc ggaagggcag ccagatcacc cagcagtcca ccaaccagag tcgcaatccc
     1081 gccaccacca accagacgga gtttgagcgc gtcttctaa
//