LOCUS AB451277 1113 bp mRNA linear HUM 02-SEP-2008 DEFINITION Homo sapiens CAMK1 mRNA for calcium/calmodulin-dependent protein kinase I, complete cds, clone: FLJ08111AAAN. ACCESSION AB451277 VERSION AB451277.1 KEYWORDS Gateway cloning system. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1113) AUTHORS Kawamura,Y., Nomura,N. and Goshima,N. TITLE Direct Submission JOURNAL Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases. Contact:Naoki Goshima Biomedicinal Information Research Center (BIRC), National Institute of Advanced Industrial Science and Technology (AIST); 2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan REFERENCE 2 AUTHORS Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R., Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T., Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C., Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M., Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K., Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A., Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y., Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T., Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H., Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T., Imai,J.-I., Watanabe,S. and Nomura,N. TITLE Human Protein Factory: an infrastructure to convert the human transcriptome into the in vitro-expressed human proteome of versatile utility JOURNAL Unpublished (2008) COMMENT This human Gateway entry clone was constructed by recombining an attB-attached open reading frame (ORF) fragment with attP sequences of the Gateway donor vector pDONR201. The ORF sequence in the entry clone is flanked with attL1 sequence (100 nt) - spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) - spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the translational initiation codon (ATG) and is also flanked with a spacer nucleotide (G) - attL2 sequence (99 nt) downstream of the ORF. This is an N-type clone which has an intrinsic stop codon at the end of ORF. DNA sequences of the entire ORF and flanking regions were validated by sequencing. Clone structure of the ORF and flanking sequences is as follows: 5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3' G acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaacgaa caggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL sequences are described in lower cases). Clone information: http://www.HGPD.jp/ This clone was produced in the "Functional Analysis of Protein and Research Application" project supported by the New Energy and Industrial Technology Development Organization (NEDO), Japan FEATURES Location/Qualifiers source 1..1113 /clone="FLJ08111AAAN" /db_xref="H-InvDB:HIT000487490" /db_xref="taxon:9606" /mol_type="mRNA" /note="Vector: pDONR201" /organism="Homo sapiens" CDS 1..1113 /codon_start=1 /gene="CAMK1" /product="calcium/calmodulin-dependent protein kinase I" /protein_id="BAG70091.1" /transl_table=1 /translation="MLGAVEGPRWKQAEDIRDIYDFRDVLGTGAFSEVILAEDKRTQK LVAIKCIAKEALEGKEGSMENEIAVLHKIKHPNIVALDDIYESGGHLYLIMQLVSGGE LFDRIVEKGFYTERDASRLIFQVLDAVKYLHDLGIVHRDLKPENLLYYSLDEDSKIMI SDFGLSKMEDPGSVLSTACGTPGYVAPEVLAQKPYSKAVDCWSIGVIAYILLCGYPPF YDENGAKLFEQILKAEYEFDSPYWDDISDSAKDFIRHLMEKDPEKRFTCEQALQHPWI AGDTALDKNIHQSVSEQIKKNFAKSKWKQAFNATAVVRHMRKLQLGTSQEGQGQTASH GELLTPVAGGPAAGCCCRDCCVEPGTELSPTLPHQL" BASE COUNT 266 a 290 c 338 g 219 t ORIGIN 1 atgctggggg cagtggaagg ccccaggtgg aagcaggcgg aggacattag agacatctac 61 gacttccgag atgttctggg cacgggggcc ttctcggagg tgatcctggc agaagataag 121 aggacgcaga agctggtggc catcaaatgc attgccaagg aggccctgga gggcaaggaa 181 ggcagcatgg agaatgagat tgctgtcctg cacaagatca agcaccccaa cattgtagcc 241 ctggatgaca tctatgagag tgggggccac ctctacctca tcatgcagct ggtgtcgggt 301 ggggagctct ttgaccgtat tgtggaaaaa ggcttctaca cggagcggga cgccagccgc 361 ctcatcttcc aggtgctgga tgctgtgaaa tacctgcatg acctgggcat tgtacaccgg 421 gatctcaagc cagagaatct gctgtactac agcctggatg aagactccaa aatcatgatc 481 tccgactttg gcctctccaa gatggaggac ccgggcagtg tgctctccac cgcctgtgga 541 actccgggat acgtggcccc tgaagtcctg gcccagaagc cctacagcaa ggctgtggat 601 tgctggtcca taggtgtcat cgcctacatc ttgctctgcg gttaccctcc cttctatgac 661 gagaatggtg ccaaactctt tgaacagatt ttgaaggccg agtacgagtt tgactctcct 721 tactgggacg acatctctga ctctgccaaa gatttcatcc ggcacttgat ggagaaggac 781 ccagagaaaa gattcacctg tgagcaggcc ttgcagcacc catggattgc aggagataca 841 gctctagata agaatatcca ccagtcggtg agtgagcaga tcaagaagaa ctttgccaag 901 agcaagtgga agcaagcctt caatgccacg gctgtggtgc ggcacatgag gaaactgcag 961 ctgggcacca gccaggaggg gcaggggcag acggcgagcc atggggagct gctgacacca 1021 gtggctgggg ggccggcagc tggctgttgc tgtcgagact gctgcgtgga gccgggcaca 1081 gaactgtccc ccacactgcc ccaccagctc taa //