LOCUS       AB451277                1113 bp    mRNA    linear   HUM 02-SEP-2008
DEFINITION  Homo sapiens CAMK1 mRNA for calcium/calmodulin-dependent protein
            kinase I, complete cds, clone: FLJ08111AAAN.
ACCESSION   AB451277
VERSION     AB451277.1
KEYWORDS    Gateway cloning system.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Kawamura,Y., Nomura,N. and Goshima,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases.
            Contact:Naoki Goshima
            Biomedicinal Information Research Center (BIRC), National
            Institute of Advanced Industrial Science and Technology (AIST);
            2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan
REFERENCE   2
  AUTHORS   Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R.,
            Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T.,
            Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C.,
            Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M.,
            Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K.,
            Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A.,
            Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y.,
            Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T.,
            Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H.,
            Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T.,
            Imai,J.-I., Watanabe,S. and Nomura,N.
  TITLE     Human Protein Factory: an infrastructure to convert the human
            transcriptome into the in vitro-expressed human proteome of
            versatile utility
  JOURNAL   Unpublished (2008)
COMMENT     This human Gateway entry clone was constructed by recombining an
            attB-attached open reading frame (ORF) fragment with attP
            sequences of the Gateway donor vector pDONR201. The ORF sequence
            in the entry clone is flanked with attL1 sequence (100 nt) -
            spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) -
            spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the
            translational initiation codon (ATG) and is also flanked with a
            spacer nucleotide (G) - attL2 sequence (99 nt) downstream of the
            ORF. This is an N-type clone which has an intrinsic stop codon at
            the end of ORF. DNA sequences of the entire ORF and flanking
            regions were validated by sequencing. Clone structure of the ORF
            and flanking sequences is as follows:
            5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct
            tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3'
            G acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaacgaa
            caggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL
            sequences are described in lower cases).
            Clone information: http://www.HGPD.jp/
            This clone was produced in the "Functional Analysis of Protein and
            Research Application" project supported by the New Energy and
            Industrial Technology Development Organization (NEDO), Japan
FEATURES             Location/Qualifiers
     source          1..1113
                     /clone="FLJ08111AAAN"
                     /db_xref="H-InvDB:HIT000487490"
                     /db_xref="taxon:9606"
                     /mol_type="mRNA"
                     /note="Vector: pDONR201"
                     /organism="Homo sapiens"
     CDS             1..1113
                     /codon_start=1
                     /gene="CAMK1"
                     /product="calcium/calmodulin-dependent protein kinase I"
                     /protein_id="BAG70091.1"
                     /transl_table=1
                     /translation="MLGAVEGPRWKQAEDIRDIYDFRDVLGTGAFSEVILAEDKRTQK
                     LVAIKCIAKEALEGKEGSMENEIAVLHKIKHPNIVALDDIYESGGHLYLIMQLVSGGE
                     LFDRIVEKGFYTERDASRLIFQVLDAVKYLHDLGIVHRDLKPENLLYYSLDEDSKIMI
                     SDFGLSKMEDPGSVLSTACGTPGYVAPEVLAQKPYSKAVDCWSIGVIAYILLCGYPPF
                     YDENGAKLFEQILKAEYEFDSPYWDDISDSAKDFIRHLMEKDPEKRFTCEQALQHPWI
                     AGDTALDKNIHQSVSEQIKKNFAKSKWKQAFNATAVVRHMRKLQLGTSQEGQGQTASH
                     GELLTPVAGGPAAGCCCRDCCVEPGTELSPTLPHQL"
BASE COUNT          266 a          290 c          338 g          219 t
ORIGIN      
        1 atgctggggg cagtggaagg ccccaggtgg aagcaggcgg aggacattag agacatctac
       61 gacttccgag atgttctggg cacgggggcc ttctcggagg tgatcctggc agaagataag
      121 aggacgcaga agctggtggc catcaaatgc attgccaagg aggccctgga gggcaaggaa
      181 ggcagcatgg agaatgagat tgctgtcctg cacaagatca agcaccccaa cattgtagcc
      241 ctggatgaca tctatgagag tgggggccac ctctacctca tcatgcagct ggtgtcgggt
      301 ggggagctct ttgaccgtat tgtggaaaaa ggcttctaca cggagcggga cgccagccgc
      361 ctcatcttcc aggtgctgga tgctgtgaaa tacctgcatg acctgggcat tgtacaccgg
      421 gatctcaagc cagagaatct gctgtactac agcctggatg aagactccaa aatcatgatc
      481 tccgactttg gcctctccaa gatggaggac ccgggcagtg tgctctccac cgcctgtgga
      541 actccgggat acgtggcccc tgaagtcctg gcccagaagc cctacagcaa ggctgtggat
      601 tgctggtcca taggtgtcat cgcctacatc ttgctctgcg gttaccctcc cttctatgac
      661 gagaatggtg ccaaactctt tgaacagatt ttgaaggccg agtacgagtt tgactctcct
      721 tactgggacg acatctctga ctctgccaaa gatttcatcc ggcacttgat ggagaaggac
      781 ccagagaaaa gattcacctg tgagcaggcc ttgcagcacc catggattgc aggagataca
      841 gctctagata agaatatcca ccagtcggtg agtgagcaga tcaagaagaa ctttgccaag
      901 agcaagtgga agcaagcctt caatgccacg gctgtggtgc ggcacatgag gaaactgcag
      961 ctgggcacca gccaggaggg gcaggggcag acggcgagcc atggggagct gctgacacca
     1021 gtggctgggg ggccggcagc tggctgttgc tgtcgagact gctgcgtgga gccgggcaca
     1081 gaactgtccc ccacactgcc ccaccagctc taa
//