LOCUS       AB451260                 741 bp    mRNA    linear   HUM 02-SEP-2008
DEFINITION  Homo sapiens YWHAB mRNA for 14-3-3 protein beta/alpha, complete
            cds, clone: FLJ08074AAAN.
ACCESSION   AB451260
VERSION     AB451260.1
KEYWORDS    Gateway cloning system.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Kawamura,Y., Nomura,N. and Goshima,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases.
            Contact:Naoki Goshima
            Biomedicinal Information Research Center (BIRC), National
            Institute of Advanced Industrial Science and Technology (AIST);
            2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan
REFERENCE   2
  AUTHORS   Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R.,
            Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T.,
            Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C.,
            Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M.,
            Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K.,
            Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A.,
            Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y.,
            Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T.,
            Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H.,
            Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T.,
            Imai,J.-I., Watanabe,S. and Nomura,N.
  TITLE     Human Protein Factory: an infrastructure to convert the human
            transcriptome into the in vitro-expressed human proteome of
            versatile utility
  JOURNAL   Unpublished (2008)
COMMENT     This human Gateway entry clone was constructed by recombining an
            attB-attached open reading frame (ORF) fragment with attP
            sequences of the Gateway donor vector pDONR201. The ORF sequence
            in the entry clone is flanked with attL1 sequence (100 nt) -
            spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) -
            spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the
            translational initiation codon (ATG) and is also flanked with a
            spacer nucleotide (G) - attL2 sequence (99 nt) downstream of the
            ORF. This is an N-type clone which has an intrinsic stop codon at
            the end of ORF. DNA sequences of the entire ORF and flanking
            regions were validated by sequencing. Clone structure of the ORF
            and flanking sequences is as follows:
            5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct
            tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3'
            G acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaacgaa
            caggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL
            sequences are described in lower cases).
            Clone information: http://www.HGPD.jp/
            This clone was produced in the "Functional Analysis of Protein and
            Research Application" project supported by the New Energy and
            Industrial Technology Development Organization (NEDO), Japan
FEATURES             Location/Qualifiers
     source          1..741
                     /clone="FLJ08074AAAN"
                     /db_xref="H-InvDB:HIT000487473"
                     /db_xref="taxon:9606"
                     /mol_type="mRNA"
                     /note="Vector: pDONR201"
                     /organism="Homo sapiens"
     CDS             1..741
                     /codon_start=1
                     /gene="YWHAB"
                     /product="14-3-3 protein beta/alpha"
                     /protein_id="BAG70074.1"
                     /transl_table=1
                     /translation="MTMDKSELVQKAKLAEQAERYDDMAAAMKAVTEQGHELSNEERN
                     LLSVAYKNVVGARRSSWRVISSIEQKTERNEKKQQMGKEYREKIEAELQDICNDVLEL
                     LDKYLIPNATQPESKVFYLKMKGDYFRYLSEVASGDNKQTTVSNSQQAYQEAFEISKK
                     EMQPTHPIRLGLALNFSVFYYEILNSPEKACSLAKMAFDEAIAELDTLNEESYKDSTL
                     IMQLLRDNLTLWTSENQGDEGDAGEGEN"
BASE COUNT          242 a          147 c          188 g          164 t
ORIGIN      
        1 atgacaatgg ataaaagtga gctggtacag aaagccaaac tcgctgagca ggctgagcga
       61 tatgatgata tggctgcagc catgaaggca gtcacagaac aggggcatga actctccaac
      121 gaagagagaa atctgctctc tgttgcctac aagaatgtgg taggcgcccg ccgctcttcc
      181 tggcgtgtca tctccagcat tgagcagaaa acagagagga atgagaagaa gcagcagatg
      241 ggcaaagagt accgtgagaa gatagaggca gaactgcagg acatctgcaa tgatgttctg
      301 gagctgttgg acaaatatct tattcccaat gctacacaac cagaaagtaa ggtgttctac
      361 ttgaaaatga aaggagatta ttttaggtat ctttctgaag tggcatctgg agacaacaaa
      421 caaaccactg tgtcgaactc ccagcaggct taccaggaag catttgaaat tagtaagaaa
      481 gaaatgcagc ctacacaccc aattcgtctt ggtctggcac taaatttctc agtcttttac
      541 tatgagattc taaactctcc tgaaaaggcc tgtagcctgg caaaaatggc atttgatgaa
      601 gcaattgctg aattggatac gctgaatgaa gagtcttata aagacagcac tctgattatg
      661 cagttactta gggacaatct cactctgtgg acatcggaaa accagggaga cgaaggagac
      721 gctggggagg gagagaacta a
//