LOCUS       AB451253                 849 bp    mRNA    linear   HUM 02-SEP-2008
DEFINITION  Homo sapiens SIAH1 mRNA for seven in absentia homolog 1 isoform a,
            complete cds, clone: FLJ08065AAAN.
ACCESSION   AB451253
VERSION     AB451253.1
KEYWORDS    Gateway cloning system.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Kawamura,Y., Nomura,N. and Goshima,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases.
            Contact:Naoki Goshima
            Biomedicinal Information Research Center (BIRC), National
            Institute of Advanced Industrial Science and Technology (AIST);
            2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan
REFERENCE   2
  AUTHORS   Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R.,
            Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T.,
            Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C.,
            Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M.,
            Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K.,
            Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A.,
            Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y.,
            Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T.,
            Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H.,
            Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T.,
            Imai,J.-I., Watanabe,S. and Nomura,N.
  TITLE     Human Protein Factory: an infrastructure to convert the human
            transcriptome into the in vitro-expressed human proteome of
            versatile utility
  JOURNAL   Unpublished (2008)
COMMENT     This human Gateway entry clone was constructed by recombining an
            attB-attached open reading frame (ORF) fragment with attP
            sequences of the Gateway donor vector pDONR201. The ORF sequence
            in the entry clone is flanked with attL1 sequence (100 nt) -
            spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) -
            spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the
            translational initiation codon (ATG) and is also flanked with a
            spacer nucleotide (G) - attL2 sequence (99 nt) downstream of the
            ORF. This is an N-type clone which has an intrinsic stop codon at
            the end of ORF. DNA sequences of the entire ORF and flanking
            regions were validated by sequencing. Clone structure of the ORF
            and flanking sequences is as follows:
            5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct
            tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3'
            G acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaacgaa
            caggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL
            sequences are described in lower cases).
            Clone information: http://www.HGPD.jp/
            This clone was produced in the "Functional Analysis of Protein and
            Research Application" project supported by the New Energy and
            Industrial Technology Development Organization (NEDO), Japan
FEATURES             Location/Qualifiers
     source          1..849
                     /clone="FLJ08065AAAN"
                     /db_xref="H-InvDB:HIT000487466"
                     /db_xref="taxon:9606"
                     /mol_type="mRNA"
                     /note="Vector: pDONR201"
                     /organism="Homo sapiens"
     CDS             1..849
                     /codon_start=1
                     /gene="SIAH1"
                     /product="seven in absentia homolog 1 isoform a"
                     /protein_id="BAG70067.1"
                     /transl_table=1
                     /translation="MSRQTATALPTGTSKCPPSQRVPALTGTNASNNDLASLFECPVC
                     FDYVLPPILQCQSGHLVCSNCRPKLTCCPTCRGPLGSIRNLAMEKVANSVLFPCKYAS
                     SGCEITLPHTEKADHEELCEFRPYSCPCPGASCKWQGSLDAVMPHLMHQHKSITTLQG
                     EDIVFLATDINLPGAVDWVMMQSCFGFHFMLVLEKQEKYDGHQQFFAIVQLIGTRKQA
                     ENFAYRLELNGHRRRLTWEATPRSIHEGIATAIMNSDCLVFDTSIAQLFAENGNLGIN
                     VTISMC"
BASE COUNT          216 a          201 c          190 g          242 t
ORIGIN      
        1 atgagccgtc agactgctac agcattacct accggtacct cgaagtgtcc accatcccag
       61 agggtgcctg ccctgactgg cacaaatgca tccaacaatg acttggcgag tctttttgag
      121 tgtccagtct gctttgacta tgtgttaccg cccattcttc aatgtcagag tggccatctt
      181 gtttgtagca actgtcgccc aaagctcaca tgttgtccaa cttgccgggg ccctttggga
      241 tccattcgca acttggctat ggagaaagtg gctaattcag tacttttccc ctgtaaatat
      301 gcgtcttctg gatgtgaaat aactctgcca cacacagaaa aagcagacca tgaagagctc
      361 tgtgagttta ggccttattc ctgtccgtgc cctggtgctt cctgtaaatg gcaaggctct
      421 ctggatgctg taatgcccca tctgatgcat cagcataagt ccattacaac cctacaggga
      481 gaggatatag tttttcttgc tacagacatt aatcttcctg gtgctgttga ctgggtgatg
      541 atgcagtcct gttttggctt tcacttcatg ttagtcttag agaaacagga aaaatacgat
      601 ggtcaccagc agttcttcgc aatcgtacag ctgataggaa cacgcaagca agctgaaaat
      661 tttgcttacc gacttgagct aaatggtcat aggcgacgat tgacttggga agcgactcct
      721 cgatctattc atgaaggaat tgcaacagcc attatgaata gcgactgtct agtctttgac
      781 accagcattg cacagctttt tgcagaaaat ggcaatttag gcatcaatgt aactatttcc
      841 atgtgttaa
//