LOCUS AB451251 408 bp mRNA linear HUM 02-SEP-2008
DEFINITION Homo sapiens SFRS6 mRNA for splicing factor arginine/serine-rich
6, complete cds, clone: FLJ08061AAAN.
ACCESSION AB451251
VERSION AB451251.1
KEYWORDS Gateway cloning system.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 408)
AUTHORS Kawamura,Y., Nomura,N. and Goshima,N.
TITLE Direct Submission
JOURNAL Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Naoki Goshima
Biomedicinal Information Research Center (BIRC), National
Institute of Advanced Industrial Science and Technology (AIST);
2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan
REFERENCE 2
AUTHORS Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R.,
Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T.,
Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C.,
Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M.,
Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K.,
Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A.,
Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y.,
Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T.,
Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H.,
Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T.,
Imai,J.-I., Watanabe,S. and Nomura,N.
TITLE Human Protein Factory: an infrastructure to convert the human
transcriptome into the in vitro-expressed human proteome of
versatile utility
JOURNAL Unpublished (2008)
COMMENT This human Gateway entry clone was constructed by recombining an
attB-attached open reading frame (ORF) fragment with attP
sequences of the Gateway donor vector pDONR201. The ORF sequence
in the entry clone is flanked with attL1 sequence (100 nt) -
spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) -
spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the
translational initiation codon (ATG) and is also flanked with a
spacer nucleotide (G) - attL2 sequence (99 nt) downstream of the
ORF. This is an N-type clone which has an intrinsic stop codon at
the end of ORF. DNA sequences of the entire ORF and flanking
regions were validated by sequencing. Clone structure of the ORF
and flanking sequences is as follows:
5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct
tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3'
G acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaacgaa
caggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL
sequences are described in lower cases).
Clone information: http://www.HGPD.jp/
This clone was produced in the "Functional Analysis of Protein and
Research Application" project supported by the New Energy and
Industrial Technology Development Organization (NEDO), Japan
FEATURES Location/Qualifiers
source 1..408
/clone="FLJ08061AAAN"
/db_xref="H-InvDB:HIT000487464"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="Vector: pDONR201"
/organism="Homo sapiens"
CDS 1..408
/codon_start=1
/gene="SFRS6"
/product="splicing factor arginine/serine-rich 6"
/protein_id="BAG70065.1"
/transl_table=1
/translation="MPRVYIGRLSYNVREKDIQRFFSGYGRLLEVDLKNGYGFVEFED
SRDADDAVYELNGKELCGERVIVEHARGPRRDRDGYSYGSRMTNGAEAVSTEAKVTAF
PDWPWLFHTLCDPCPMTLWLTLPEAMTTAAFCH"
BASE COUNT 81 a 121 c 121 g 85 t
ORIGIN
1 atgccgcgcg tctacatagg acgcctgagc tacaacgtcc gggagaagga catccagcgc
61 tttttcagtg gctatggccg cctcctcgaa gtagacctca aaaatgggta cggcttcgtg
121 gagttcgagg actcccgcga cgccgacgac gccgtttacg agctgaacgg caaggagctc
181 tgcggcgagc gcgtgatcgt agagcacgcc cggggcccgc gtcgcgatcg cgacggctac
241 agctacggaa gccgcatgac caatggggct gaggctgtgt ccactgaggc taaggtgact
301 gcctttcctg attggccttg gcttttccat acattgtgtg acccttgccc tatgaccctt
361 tggctgacct taccggaagc catgacgaca gcagcctttt gccattaa
//