LOCUS       AB451245                 678 bp    mRNA    linear   HUM 02-SEP-2008
DEFINITION  Homo sapiens BCAS2 mRNA for breast carcinoma amplified sequence 2,
            complete cds, clone: FLJ08051AAAN.
ACCESSION   AB451245
VERSION     AB451245.1
KEYWORDS    Gateway cloning system.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Kawamura,Y., Nomura,N. and Goshima,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases.
            Contact:Naoki Goshima
            Biomedicinal Information Research Center (BIRC), National
            Institute of Advanced Industrial Science and Technology (AIST);
            2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan
REFERENCE   2
  AUTHORS   Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R.,
            Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T.,
            Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C.,
            Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M.,
            Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K.,
            Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A.,
            Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y.,
            Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T.,
            Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H.,
            Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T.,
            Imai,J.-I., Watanabe,S. and Nomura,N.
  TITLE     Human Protein Factory: an infrastructure to convert the human
            transcriptome into the in vitro-expressed human proteome of
            versatile utility
  JOURNAL   Unpublished (2008)
COMMENT     This human Gateway entry clone was constructed by recombining an
            attB-attached open reading frame (ORF) fragment with attP
            sequences of the Gateway donor vector pDONR201. The ORF sequence
            in the entry clone is flanked with attL1 sequence (100 nt) -
            spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) -
            spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the
            translational initiation codon (ATG) and is also flanked with a
            spacer nucleotide (G) - attL2 sequence (99 nt) downstream of the
            ORF. This is an N-type clone which has an intrinsic stop codon at
            the end of ORF. DNA sequences of the entire ORF and flanking
            regions were validated by sequencing. Clone structure of the ORF
            and flanking sequences is as follows:
            5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct
            tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3'
            G acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaacgaa
            caggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL
            sequences are described in lower cases).
            Clone information: http://www.HGPD.jp/
            This clone was produced in the "Functional Analysis of Protein and
            Research Application" project supported by the New Energy and
            Industrial Technology Development Organization (NEDO), Japan
FEATURES             Location/Qualifiers
     source          1..678
                     /clone="FLJ08051AAAN"
                     /db_xref="H-InvDB:HIT000487458"
                     /db_xref="taxon:9606"
                     /mol_type="mRNA"
                     /note="Vector: pDONR201"
                     /organism="Homo sapiens"
     CDS             1..678
                     /codon_start=1
                     /gene="BCAS2"
                     /product="breast carcinoma amplified sequence 2"
                     /protein_id="BAG70059.1"
                     /transl_table=1
                     /translation="MAGTGLVAGEVVVDALPYFDQGYEAPGVREAAAALVEEETRRYR
                     PTKNYLSYLTAPDYSAFETDIMRNEFERLAARQPIELLSMKRYELPAPSSGQKNDITA
                     WQECVNNSMAQLEHQAVRIENLELMSQHGCNAWKVYNENLVHMIEHAQKELQKLRKHI
                     QDLNWQRKNMQLTAGSKLREMESNWVSLVSKNYEIERTIVQLENEIYQIKQQHGEANK
                     ENIRQDF"
BASE COUNT          229 a          126 c          166 g          157 t
ORIGIN      
        1 atggcgggca caggtttggt ggctggagag gttgtggtgg atgcgctgcc gtattttgat
       61 caaggttatg aagcccctgg tgtgcgggaa gcggctgcag cgctggtgga ggaggaaact
      121 cgcagatacc ggcctactaa gaactacctg agctacctga cagccccgga ttattctgcc
      181 tttgaaactg acataatgag aaatgaattt gaaagactgg ctgctcgaca accaattgaa
      241 ttgctcagta tgaaacgata tgagcttcca gccccctcct ctggtcaaaa aaatgacatt
      301 actgcatggc aagaatgtgt aaacaattct atggcccagt tagagcatca agcagttaga
      361 attgagaatc tggaactaat gtcacagcat ggatgtaatg cctggaaagt atacaatgaa
      421 aatctagttc atatgattga acacgcacag aaggaacttc agaagttaag aaaacatatt
      481 caagatttaa actggcagag aaagaacatg caactcacag ctggatctaa attgagagaa
      541 atggagtcaa attgggtatc cctggtcagt aagaattatg agattgaacg gactattgtt
      601 cagctagaaa atgaaatcta tcaaattaag cagcaacatg gagaggcaaa caaagaaaac
      661 atccggcaag acttctaa
//