LOCUS       AB451237                1002 bp    mRNA    linear   HUM 02-SEP-2008
DEFINITION  Homo sapiens EIF2S2 mRNA for eukaryotic translation initiation
            factor 2 beta, complete cds, clone: FLJ08035AAAN.
ACCESSION   AB451237
VERSION     AB451237.1
KEYWORDS    Gateway cloning system.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Kawamura,Y., Nomura,N. and Goshima,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases.
            Contact:Naoki Goshima
            Biomedicinal Information Research Center (BIRC), National
            Institute of Advanced Industrial Science and Technology (AIST);
            2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan
REFERENCE   2
  AUTHORS   Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R.,
            Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T.,
            Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C.,
            Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M.,
            Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K.,
            Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A.,
            Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y.,
            Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T.,
            Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H.,
            Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T.,
            Imai,J.-I., Watanabe,S. and Nomura,N.
  TITLE     Human Protein Factory: an infrastructure to convert the human
            transcriptome into the in vitro-expressed human proteome of
            versatile utility
  JOURNAL   Unpublished (2008)
COMMENT     This human Gateway entry clone was constructed by recombining an
            attB-attached open reading frame (ORF) fragment with attP
            sequences of the Gateway donor vector pDONR201. The ORF sequence
            in the entry clone is flanked with attL1 sequence (100 nt) -
            spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) -
            spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the
            translational initiation codon (ATG) and is also flanked with a
            spacer nucleotide (G) - attL2 sequence (99 nt) downstream of the
            ORF. This is an N-type clone which has an intrinsic stop codon at
            the end of ORF. DNA sequences of the entire ORF and flanking
            regions were validated by sequencing. Clone structure of the ORF
            and flanking sequences is as follows:
            5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct
            tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3'
            G acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaacgaa
            caggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL
            sequences are described in lower cases).
            Clone information: http://www.HGPD.jp/
            This clone was produced in the "Functional Analysis of Protein and
            Research Application" project supported by the New Energy and
            Industrial Technology Development Organization (NEDO), Japan
FEATURES             Location/Qualifiers
     source          1..1002
                     /clone="FLJ08035AAAN"
                     /db_xref="H-InvDB:HIT000487450"
                     /db_xref="taxon:9606"
                     /mol_type="mRNA"
                     /note="Vector: pDONR201"
                     /organism="Homo sapiens"
     CDS             1..1002
                     /codon_start=1
                     /gene="EIF2S2"
                     /product="eukaryotic translation initiation factor 2 beta"
                     /protein_id="BAG70051.1"
                     /transl_table=1
                     /translation="MSGDEMIFDPTMSKKKKKKKKPFMLDEEGDTQTEETQPSETKEV
                     EPEPTEDKDLEADEEDTRKKDASDDLDDLNFFNQKKKKKKTKKIFDIDEAEEGVKDLK
                     IESGVQEPTEPEDDLDIMLGNKKKKKKNVKFPDEDEILEKDEALEDEDNKKDDGISFS
                     NQTGPAWAGSERDYTYEELLNRVFNIMREKNPDMVAGEKRKFVMKPPQVVRVGTKKTS
                     FVNFTDICKLLHRQPKHLLAFLLAELGTSGSIDGNNQLVIKGRFQQKQIENVLRRYIK
                     EYVTCHTCRSPDTILQKDTRLYFLQCETCHSRCSVASIKTGFQAVTGKRAQLRAKAN"
BASE COUNT          373 a          173 c          230 g          226 t
ORIGIN      
        1 atgtctgggg acgagatgat ttttgatcct actatgagca agaagaaaaa gaagaagaag
       61 aagcctttta tgttagatga ggaaggggat acccaaacag aggaaaccca gccttcagaa
      121 acaaaagaag tggagccaga gccaactgag gacaaggatt tggaagctga tgaagaggac
      181 actaggaaaa aagatgcttc tgatgatcta gatgacttga acttctttaa tcaaaagaaa
      241 aagaagaaaa aaactaaaaa gatatttgat attgatgaag ctgaagaagg tgtaaaggat
      301 cttaagattg aaagtggtgt tcaagaacca actgaaccag aggatgacct tgacattatg
      361 cttggcaata aaaagaagaa aaagaagaat gttaagttcc cagatgagga tgaaatacta
      421 gagaaagatg aagctctaga agatgaagac aacaaaaaag atgatggtat ctcattcagt
      481 aatcagacag gccctgcttg ggcaggctca gaaagagact acacatatga ggagctgctg
      541 aatcgagtgt tcaacatcat gagggaaaag aatccagata tggttgctgg ggagaaaagg
      601 aaatttgtca tgaaacctcc acaagtcgtc cgagtaggaa ccaagaaaac ttcttttgtc
      661 aactttacag atatctgtaa actattacat cgtcagccca aacatctcct tgcatttttg
      721 ttggctgaat tgggtacaag tggttctata gatggtaata accaacttgt aatcaaagga
      781 agattccaac agaaacagat agaaaatgtc ttgagaagat atatcaagga atatgtcact
      841 tgtcacacat gccgatcacc ggacacaatc ctgcagaagg acacacgact ctatttccta
      901 cagtgcgaaa cttgtcattc tagatgttct gttgccagta tcaaaaccgg cttccaggct
      961 gtcacgggca agcgagcaca gctccgtgcc aaagctaact aa
//