LOCUS AB451237 1002 bp mRNA linear HUM 02-SEP-2008 DEFINITION Homo sapiens EIF2S2 mRNA for eukaryotic translation initiation factor 2 beta, complete cds, clone: FLJ08035AAAN. ACCESSION AB451237 VERSION AB451237.1 KEYWORDS Gateway cloning system. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1002) AUTHORS Kawamura,Y., Nomura,N. and Goshima,N. TITLE Direct Submission JOURNAL Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases. Contact:Naoki Goshima Biomedicinal Information Research Center (BIRC), National Institute of Advanced Industrial Science and Technology (AIST); 2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan REFERENCE 2 AUTHORS Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R., Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T., Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C., Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M., Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K., Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A., Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y., Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T., Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H., Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T., Imai,J.-I., Watanabe,S. and Nomura,N. TITLE Human Protein Factory: an infrastructure to convert the human transcriptome into the in vitro-expressed human proteome of versatile utility JOURNAL Unpublished (2008) COMMENT This human Gateway entry clone was constructed by recombining an attB-attached open reading frame (ORF) fragment with attP sequences of the Gateway donor vector pDONR201. The ORF sequence in the entry clone is flanked with attL1 sequence (100 nt) - spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) - spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the translational initiation codon (ATG) and is also flanked with a spacer nucleotide (G) - attL2 sequence (99 nt) downstream of the ORF. This is an N-type clone which has an intrinsic stop codon at the end of ORF. DNA sequences of the entire ORF and flanking regions were validated by sequencing. Clone structure of the ORF and flanking sequences is as follows: 5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3' G acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaacgaa caggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL sequences are described in lower cases). Clone information: http://www.HGPD.jp/ This clone was produced in the "Functional Analysis of Protein and Research Application" project supported by the New Energy and Industrial Technology Development Organization (NEDO), Japan FEATURES Location/Qualifiers source 1..1002 /clone="FLJ08035AAAN" /db_xref="H-InvDB:HIT000487450" /db_xref="taxon:9606" /mol_type="mRNA" /note="Vector: pDONR201" /organism="Homo sapiens" CDS 1..1002 /codon_start=1 /gene="EIF2S2" /product="eukaryotic translation initiation factor 2 beta" /protein_id="BAG70051.1" /transl_table=1 /translation="MSGDEMIFDPTMSKKKKKKKKPFMLDEEGDTQTEETQPSETKEV EPEPTEDKDLEADEEDTRKKDASDDLDDLNFFNQKKKKKKTKKIFDIDEAEEGVKDLK IESGVQEPTEPEDDLDIMLGNKKKKKKNVKFPDEDEILEKDEALEDEDNKKDDGISFS NQTGPAWAGSERDYTYEELLNRVFNIMREKNPDMVAGEKRKFVMKPPQVVRVGTKKTS FVNFTDICKLLHRQPKHLLAFLLAELGTSGSIDGNNQLVIKGRFQQKQIENVLRRYIK EYVTCHTCRSPDTILQKDTRLYFLQCETCHSRCSVASIKTGFQAVTGKRAQLRAKAN" BASE COUNT 373 a 173 c 230 g 226 t ORIGIN 1 atgtctgggg acgagatgat ttttgatcct actatgagca agaagaaaaa gaagaagaag 61 aagcctttta tgttagatga ggaaggggat acccaaacag aggaaaccca gccttcagaa 121 acaaaagaag tggagccaga gccaactgag gacaaggatt tggaagctga tgaagaggac 181 actaggaaaa aagatgcttc tgatgatcta gatgacttga acttctttaa tcaaaagaaa 241 aagaagaaaa aaactaaaaa gatatttgat attgatgaag ctgaagaagg tgtaaaggat 301 cttaagattg aaagtggtgt tcaagaacca actgaaccag aggatgacct tgacattatg 361 cttggcaata aaaagaagaa aaagaagaat gttaagttcc cagatgagga tgaaatacta 421 gagaaagatg aagctctaga agatgaagac aacaaaaaag atgatggtat ctcattcagt 481 aatcagacag gccctgcttg ggcaggctca gaaagagact acacatatga ggagctgctg 541 aatcgagtgt tcaacatcat gagggaaaag aatccagata tggttgctgg ggagaaaagg 601 aaatttgtca tgaaacctcc acaagtcgtc cgagtaggaa ccaagaaaac ttcttttgtc 661 aactttacag atatctgtaa actattacat cgtcagccca aacatctcct tgcatttttg 721 ttggctgaat tgggtacaag tggttctata gatggtaata accaacttgt aatcaaagga 781 agattccaac agaaacagat agaaaatgtc ttgagaagat atatcaagga atatgtcact 841 tgtcacacat gccgatcacc ggacacaatc ctgcagaagg acacacgact ctatttccta 901 cagtgcgaaa cttgtcattc tagatgttct gttgccagta tcaaaaccgg cttccaggct 961 gtcacgggca agcgagcaca gctccgtgcc aaagctaact aa //