LOCUS AB451230 1419 bp mRNA linear HUM 02-SEP-2008
DEFINITION Homo sapiens EIF2S3 mRNA for eukaryotic translation initiation
factor 2 subunit 3, complete cds, clone: FLJ08020AAAN.
ACCESSION AB451230
VERSION AB451230.1
KEYWORDS Gateway cloning system.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1419)
AUTHORS Kawamura,Y., Nomura,N. and Goshima,N.
TITLE Direct Submission
JOURNAL Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Naoki Goshima
Biomedicinal Information Research Center (BIRC), National
Institute of Advanced Industrial Science and Technology (AIST);
2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan
REFERENCE 2
AUTHORS Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R.,
Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T.,
Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C.,
Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M.,
Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K.,
Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A.,
Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y.,
Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T.,
Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H.,
Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T.,
Imai,J.-I., Watanabe,S. and Nomura,N.
TITLE Human Protein Factory: an infrastructure to convert the human
transcriptome into the in vitro-expressed human proteome of
versatile utility
JOURNAL Unpublished (2008)
COMMENT This human Gateway entry clone was constructed by recombining an
attB-attached open reading frame (ORF) fragment with attP
sequences of the Gateway donor vector pDONR201. The ORF sequence
in the entry clone is flanked with attL1 sequence (100 nt) -
spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) -
spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the
translational initiation codon (ATG) and is also flanked with a
spacer nucleotide (G) - attL2 sequence (99 nt) downstream of the
ORF. This is an N-type clone which has an intrinsic stop codon at
the end of ORF. DNA sequences of the entire ORF and flanking
regions were validated by sequencing. Clone structure of the ORF
and flanking sequences is as follows:
5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct
tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3'
G acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaacgaa
caggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL
sequences are described in lower cases).
Clone information: http://www.HGPD.jp/
This clone was produced in the "Functional Analysis of Protein and
Research Application" project supported by the New Energy and
Industrial Technology Development Organization (NEDO), Japan
FEATURES Location/Qualifiers
source 1..1419
/clone="FLJ08020AAAN"
/db_xref="H-InvDB:HIT000487443"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="Vector: pDONR201"
/organism="Homo sapiens"
CDS 1..1419
/codon_start=1
/gene="EIF2S3"
/product="eukaryotic translation initiation factor 2
subunit 3"
/protein_id="BAG70044.1"
/transl_table=1
/translation="MAGGEAGVTLGQPHLSRQDLTTLDVTKLTPLSHEVISRQATINI
GTIGHVAHGKSTVVKAISGVHTVRFKNELERNITIKLGYANAKIYKLDDPSCPRPECY
RSCGSSTPDEFPTDIPGTKGNFKLVRHVSFVDCPGHDILMATMLNGAAVMDAALLLIA
GNESCPQPQTSEHLAAIEIMKLKHILILQNKIDLVKESQAKEQYEQILAFVQGTVAEG
APIIPISAQLKYNIEVVCEYIVKKIPVPPRDFTSEPRLIVIRSFDVNKPGCEVDDLKG
GVAGGSILKGVLKVGQEIEVRPGIVSKDSEGKLMCKPIFSKIVSLFAEHNDLQYAAPG
GLIGVGTKIDPTLCRADRMVGQVLGAVGALPEIFTELEISYFLLRRLLGVRTEGDKKA
AKVQKLSKNEVLMVNIGSLSTGGRVSAVKADLGKIVLTNPVCTEVGEKIALSRRVEKH
WRLIGWGQIRRGVTIKPTVDDD"
BASE COUNT 431 a 266 c 346 g 376 t
ORIGIN
1 atggcgggcg gagaagctgg agtgactcta gggcagccgc atctttcgcg tcaggatctc
61 accaccttgg atgttaccaa gttgacgcca ctttcacatg aagttatcag cagacaagcc
121 acaattaaca taggtacaat tggtcatgta gctcatggga aatccacagt cgtcaaagct
181 atttctggag ttcatactgt caggttcaaa aatgaactag aaagaaatat tacaatcaag
241 cttggatatg ctaatgctaa gatttataag cttgatgacc caagttgccc tcggccagaa
301 tgttatagat cttgtgggag cagtacacct gacgagtttc ctacggacat tccagggacc
361 aaagggaact tcaaattagt cagacatgtt tcctttgttg actgtcctgg ccacgatatt
421 ttgatggcta ctatgctgaa cggtgcagca gtgatggatg cagctcttct gttgatagct
481 ggtaatgaat cttgccctca gcctcagaca tcggaacacc tggctgctat agagatcatg
541 aaactgaagc atattttgat tctacaaaat aaaattgatt tggtaaaaga aagtcaggct
601 aaagaacaat acgagcagat ccttgcattt gtccaaggta cagtagcaga gggagctccc
661 attattccaa tttcagctca gctgaaatac aatattgaag ttgtttgtga gtacatagta
721 aagaaaattc cagtaccccc aagagacttt acttcagagc cccggcttat tgttattaga
781 tcttttgatg tcaacaaacc tggctgtgaa gttgatgacc ttaagggagg tgtagctggt
841 ggtagtatcc taaaaggagt attaaaggtg ggccaggaga tagaagtaag acctggtatt
901 gtttccaaag atagtgaagg aaaactcatg tgtaaaccaa tcttttccaa aattgtatca
961 ctttttgcgg agcataatga tctgcaatat gctgctccag gcggtcttat tggagttgga
1021 acaaaaattg accccacttt gtgccgggct gacagaatgg tggggcaagt acttggtgca
1081 gtcggagctt tacctgagat attcacagaa ttggaaattt cctatttcct gcttagacgg
1141 cttctaggtg tacgcactga aggagacaag aaagcagcaa aggttcaaaa gctgtctaag
1201 aatgaagtgc tcatggtgaa cataggatcc ctgtcaacag gagggagagt tagtgctgtc
1261 aaggccgatt tgggtaaaat tgttttgacc aatccagtgt gcacagaggt aggagaaaaa
1321 attgccctta gccgaagagt tgaaaaacac tggcgtttaa ttggttgggg tcagataaga
1381 agaggagtga caatcaagcc aacagtagat gatgactaa
//