LOCUS       AB451221                1098 bp    mRNA    linear   HUM 02-SEP-2008
DEFINITION  Homo sapiens MAPK13 mRNA for mitogen-activated protein kinase 13,
            complete cds, clone: FLJ08003AAAN.
ACCESSION   AB451221
VERSION     AB451221.1
KEYWORDS    Gateway cloning system.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Kawamura,Y., Nomura,N. and Goshima,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases.
            Contact:Naoki Goshima
            Biomedicinal Information Research Center (BIRC), National
            Institute of Advanced Industrial Science and Technology (AIST);
            2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan
REFERENCE   2
  AUTHORS   Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R.,
            Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T.,
            Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C.,
            Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M.,
            Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K.,
            Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A.,
            Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y.,
            Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T.,
            Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H.,
            Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T.,
            Imai,J.-I., Watanabe,S. and Nomura,N.
  TITLE     Human Protein Factory: an infrastructure to convert the human
            transcriptome into the in vitro-expressed human proteome of
            versatile utility
  JOURNAL   Unpublished (2008)
COMMENT     This human Gateway entry clone was constructed by recombining an
            attB-attached open reading frame (ORF) fragment with attP
            sequences of the Gateway donor vector pDONR201. The ORF sequence
            in the entry clone is flanked with attL1 sequence (100 nt) -
            spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) -
            spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the
            translational initiation codon (ATG) and is also flanked with a
            spacer nucleotide (G) - attL2 sequence (99 nt) downstream of the
            ORF. This is an N-type clone which has an intrinsic stop codon at
            the end of ORF. DNA sequences of the entire ORF and flanking
            regions were validated by sequencing. Clone structure of the ORF
            and flanking sequences is as follows:
            5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct
            tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3'
            G acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaacgaa
            caggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL
            sequences are described in lower cases).
            Clone information: http://www.HGPD.jp/
            This clone was produced in the "Functional Analysis of Protein and
            Research Application" project supported by the New Energy and
            Industrial Technology Development Organization (NEDO), Japan
FEATURES             Location/Qualifiers
     source          1..1098
                     /clone="FLJ08003AAAN"
                     /db_xref="H-InvDB:HIT000487434"
                     /db_xref="taxon:9606"
                     /mol_type="mRNA"
                     /note="Vector: pDONR201"
                     /organism="Homo sapiens"
     CDS             1..1098
                     /codon_start=1
                     /gene="MAPK13"
                     /product="mitogen-activated protein kinase 13"
                     /protein_id="BAG70035.1"
                     /transl_table=1
                     /translation="MSLIRKKGFYKQDVNKTAWELPKTYVSPTHVGSGAYDSVCSAID
                     KRSGEKVAIKKLSRPFQSEIFAKRAYRELLLLKHMQHENVIGLLDVFTPASSLRNFYD
                     FYLVMPFMQTDLQKIMGMEFSEEKIQYLVYQMLKGLKYIHSAGVVHRDLKPGNLAVNE
                     DCELKILDFGLARHADAEMTGYVVTRWYRAPEVILSWMHYNQTVDIWSVGCIMAEMLT
                     GKTLFKGKDYLDQLTQILKVTGVPGTEFVQKLNDKAAKSYIQSLPQTPRKDFTQLFPR
                     ASPQAADLLEKMLELDVDKRLTAAQALTHPFFEPFRDPEEETEAQQPFDDSLEHEKLT
                     VDEWKQHIYKEIVNFSPIARKDSRRRSGMKL"
BASE COUNT          262 a          310 c          322 g          204 t
ORIGIN      
        1 atgagcctca tccggaaaaa gggcttctac aagcaggacg tcaacaagac cgcctgggag
       61 ctgcccaaga cctacgtgtc cccgacgcac gtcggcagcg gggcctatga ctccgtgtgc
      121 tcggccatcg acaagcggtc aggggagaag gtggccatca agaagctgag ccgacccttt
      181 cagtccgaga tcttcgccaa gcgcgcctac cgggagctgc tgctgctgaa gcacatgcag
      241 catgagaacg tcattgggct cctggatgtc ttcaccccag cctcctccct gcgcaacttc
      301 tatgacttct acctggtgat gcccttcatg cagacggatc tgcagaagat catggggatg
      361 gagttcagtg aggagaagat ccagtacctg gtgtatcaga tgctcaaagg ccttaagtac
      421 atccactctg ctggggtcgt gcacagggac ctgaagccag gcaacctggc tgtgaatgag
      481 gactgtgaac tgaagattct ggattttggg ctggcgcgac atgcagacgc cgagatgact
      541 ggctacgtgg tgacccgctg gtaccgagcc cccgaggtga tcctcagctg gatgcactac
      601 aaccagacag tggacatctg gtctgtgggc tgtatcatgg cagagatgct gacagggaaa
      661 actctgttca aggggaaaga ttacctggac cagctgaccc agatcctgaa agtgaccggg
      721 gtgcctggca cggagtttgt gcagaagctg aacgacaaag cggccaaatc ctacatccag
      781 tccctgccac agacccccag gaaggatttc actcagctgt tcccacgggc cagcccccag
      841 gctgcggacc tgctggagaa gatgctggag ctagacgtgg acaagcgcct gacggccgcg
      901 caggccctca cccatccctt ctttgaaccc ttccgggacc ctgaggaaga gacggaggcc
      961 cagcagccgt ttgatgattc cttagaacac gagaaactca cagtggatga atggaagcag
     1021 cacatctaca aggagattgt gaacttcagc cccattgccc ggaaggactc acggcgccgg
     1081 agtggcatga agctgtaa
//