LOCUS AB220420 1837 bp mRNA linear PRI 06-OCT-2006
DEFINITION Macaca fascicularis mRNA, clone QccE-14817: similar to Homo
sapiens calmodulin 1 (phosphorylase kinase, delta) (CALM1), mRNA,
NM_006888.2.
ACCESSION AB220420
VERSION AB220420.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Macaca fascicularis (crab-eating macaque)
ORGANISM Macaca fascicularis
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Cercopithecidae; Cercopithecinae; Macaca.
REFERENCE 1 (bases 1 to 1837)
AUTHORS Hashimoto,K., Osada,N. and Kusuda,J.
TITLE Direct Submission
JOURNAL Submitted (04-JUL-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Katsuyuki Hashimoto
National Institute of Biomedical Innovation, Division of
Biomedical Resources; 7-6-8, Saito-Asagi, Ibaraki, Osaka 567-0085,
Japan
REFERENCE 2
AUTHORS Kobayashi,M., Tanuma,R., Hirata,M., Osada,N., Kusuda,J., Sugano,S.
and Hashimoto,K.
TITLE Analysis of gene expression in cynomolgus monkey tissues by
macaque cDNA oligo-chips
JOURNAL Unpublished (2005)
COMMENT Lab host: TOP10
Vector: pME18S-FL3 (Acc.No. AB009864)
R. Site1: DraIII (CACTGTGTG)
R. Site2: DraIII (CACCATGTG)
Description: 1st strand cDNA was primed with an oligo(dT) primer
[ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was
synthesized using specific 5' and 3' primers and amplified
by PCR. The PCR product was digested with SfiI and size selection
was performed to exclude fragments <1.5kb.The SfiI-digested
PCR product was cloned into distinct DraIII sites of pME18S-FL3.
XhoI sites just outside the DraIII sites can be used to
isolate the cDNA insert. Libraries were constructed by
oligo-capping method. Libraries were made from:
QccE: cerebellum cortex
QnpA: parietal lobe
QtrA: temporal lobe right
QflA: frontal lobe left
QmoA: medulla oblongata
QbsA(B): brain stem
QorA: occipital lobe right
QtsA: testis
Custom primers were used for 5' and 3'-end sequencing.
The full-insert sequencing was done by primer-walking method using
ABI DNA sequencer.
FEATURES Location/Qualifiers
source 1..1837
/clone="QccE-14817"
/clone_lib="macaque brain cDNA library QccE"
/db_xref="taxon:9541"
/dev_stage="adult"
/mol_type="mRNA"
/organism="Macaca fascicularis"
/sex="male"
/tissue_type="cerebellum cortex"
CDS 206..655
/codon_start=1
/note="similar to Homo sapiens calmodulin 1
(phosphorylase kinase, delta) (CALM1), mRNA, NM_006888.2."
/product="hypothetical protein"
/protein_id="BAE72953.1"
/transl_table=1
/translation="MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNP
TEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYIS
AAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
polyA_site 1821..1837
BASE COUNT 546 a 395 c 417 g 479 t
ORIGIN
1 agtggtgctg gaagtcttgt ggacgccgtg ccgttactcg tagtcaggcg gcggcgcagg
61 cggcggcggc ggcacagcgc acagcgcgcc ttagcagcag cagcagcggc agcagcagca
121 ccatcggagg cacccccgcc gtcacagccc ccgtgctggt gcagccaccc tcgctccctc
181 tgctcttcct cccttcgctc gcaccatggc tgatcagctg accgaggaac agattgctga
241 attcaaggaa gctttctccc tattcgataa ggatggcgat ggcaccatca caacaaagga
301 acttggaact gtcatgaggt cactgggtca gaacccaaca gaagctgaat tgcaggatat
361 gatcaacgaa gtggatgctg atggtaatgg caccattgac ttccctgaat ttttgactat
421 gatggctaga aaaatgaaag atacagatag tgaagaagaa atccgtgagg cattccgagt
481 ctttgacaag gatggcaatg gttacatcag tgcagcagaa ctacgtcacg tcatgacaaa
541 cttaggagaa aaactaacag atgaagaagt agatgaaatg atcagagaag cagatattga
601 tggagacgga caagtcaact atgaagaatt cgtacagatg atgactgcaa aatgaagacc
661 tactttcaac tcctttttcc cccctctaga agaatcaaat tgaatctttt acttacctct
721 tgcaaaaaaa agaaaaaaga aaaaagttca tttattcatt ctgtttctat atagcaaaac
781 tgaatgtcaa aagtaccttc tgtccacaca cacaaaatct gcatgtattg gttggtggtc
841 ctgtccccta aagatcaagc tacacatcag ttttacaata taaatgcttg tactacctta
901 atgataagga ctccttaaag ttccatttgc taatgataca ctgtttgggc tggccagttt
961 ttcatgcatg cagcttgacg attgagcaca gtcaggcctt tgtattaaaa atgaaaaatg
1021 aaaaaaattc aaaacctatt caaatgggtt ctagttcaat ttgtttagta taaattgtca
1081 tagctggttt actgaaaaca aacacattta aaattggttt acctcaggat gacgtgcaga
1141 aaaatgggtg aaggataaac cgttgagaca tggccccact ggtaggatgg tcctcttgta
1201 cttcgtgtgc tccgacccgt ggtgacgaca caccctggtg gcatgcctgt gtttgttggt
1261 ttagcgttgt ctgcatcgtt ctagggtgaa acaggtgtca ggctgtcact gttcacacac
1321 atttttaata agaaaccttt accaagggag catctttgga ctctctcttt ttaaaacctt
1381 ctgaacgatg acttggagcc ggcagagtag gctgtggctg tggacctcag cacaaccatc
1441 aacattgctg ttcaaagaaa ttacaattta cgtccattcc aagttgtaaa tgctagtctt
1501 tttttttttt tttttccaat aaaaagacca ttaacttaaa gtggtgttaa atgctttgta
1561 aagctgagat ctagatgggg acaaggcagg tggaggggag gccagtgtac atttaaatgc
1621 ccacagccca gcattgggtt tccctcccaa ggccccagca ccaacctctg agcccaagac
1681 cttgcctaaa aacaagcaga taccgattgc ttcatcctat ttatggacat gtaggtctag
1741 ttgcattttc actgggggga ggggggaagg tgaattacgg taacttttaa tgatctattc
1801 aggcagtaga gctcttaagg aaaaaaaaaa aaaaaaa
//