LOCUS AB179174 2572 bp mRNA linear PRI 06-OCT-2006
DEFINITION Macaca fascicularis testis cDNA clone: QtsA-14116, similar to
human CDC42 binding protein kinase alpha (DMPK-like)(CDC42BPA),
transcript variant A, mRNA, RefSeq: NM_014826.3.
ACCESSION AB179174
VERSION AB179174.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Macaca fascicularis (crab-eating macaque)
ORGANISM Macaca fascicularis
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Cercopithecidae; Cercopithecinae; Macaca.
REFERENCE 1 (bases 1 to 2572)
AUTHORS Hashimoto,K., Kusuda,J. and Sugano,S.
TITLE Direct Submission
JOURNAL Submitted (10-MAY-2004) to the DDBJ/EMBL/GenBank databases.
Contact:Katsuyuki Hashimoto
National Institute of Infectious Diseases, Division of Genetic
Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan
URL :http://www.nih.go.jp/yoken/genebank/
REFERENCE 2
AUTHORS
CONSRTM International consortium for macaque cDNA sequencing and analysis
TITLE DNA sequences of macaque genes expressed in brain or testis and
its evolutionary implications
JOURNAL Unpublished (2004)
REFERENCE 3
AUTHORS Osada,N., Hirata,M., Tanuma,R., Kusuda,J., Hida,M., Suzuki,Y.,
Sugano,S., Gojobori,T., Shen,C.-K.J., Wu,C.I. and Hashimoto,K.
TITLE Substitution Rate and Structural Divergence of 5'UTR Evolution:
Comparative Analysis Between Human and Cynomolgus Monkey cDNAs
JOURNAL Mol. Biol. Evol. 22, 1976-1982 (2005)
COMMENT The International consortium for macaque cDNA sequencing
and analysis consists of:Department of Virology and Human
Genome Center, Institute of Medical Science, The University of
Tokyo,
Tokyo, Japan; Division of Genetic Resources, National Institute
of Infectious Diseases of Japan, Tokyo, Japan; National Health
Research Institute, Taipei, Taiwan; Institute of Molecular Biology,
Academia Sinica, Taipei, Taiwan; Department of Ecology &
Evolution,
University of Chicago, Chicago, IL, USA, Center for Information
Biology,
National Institute of Genetics of Japan, Mishima, Japan.
Clone distribution: clone distribution information can be
found at: http://www.nih.go.jp/yoken/genebank/
Lab host: TOP10
Vector: pME18S-FL3 (Acc.No. AB009864)
R. Site1: DraIII (CACTGTGTG)
R. Site2: DraIII (CACCATGTG)
Description: 1st strand cDNA was primed with an oligo(dT) primer
[ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was
synthesized using specific 5' and 3' primers and amplified
by PCR. The PCR product was digested with SfiI and size selection
was performed to exclude fragments <1.5kb.The SfiI-digested
PCR product was cloned into distinct DraIII sites of pME18S-FL3.
XhoI sites just outside the DraIII sites can be used to
isolate the cDNA insert. Libraries were constructed by
oligo-capping method. Libraries were made from:
QccE: cerebellum cortex
QnpA: parietal lobe
QtrA: temporal lobe right
QflA: frontal lobe left
QmoA: medulla oblongata
QbsA: brain stem
QorA: occipital lobe right
QtsA: testis
Custom primers were used for 5' and 3'-end sequencing. The
full-insert
sequencing was done by primer-walking method using ABI DNA
sequencer.
FEATURES Location/Qualifiers
source 1..2572
/clone="QtsA-14116"
/clone_lib="macaque cDNA library QtsA"
/db_xref="taxon:9541"
/dev_stage="adult"
/mol_type="mRNA"
/organism="Macaca fascicularis"
/sex="male"
CDS 608..2119
/note="Homo sapiens CDC42 binding protein kinase alpha
(DMPK-like)(CDC42BPA), transcript variant A, mRNA,
RefSeq: NM_014826.3"
/protein_id="BAE02225.1"
/translation="MRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSI
LMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIID
HERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPL
SALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKF
KEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDHTLSFIAHQPMD
AICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENA
VDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETS
DNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGP
GDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSANG"
BASE COUNT 793 a 539 c 548 g 692 t
ORIGIN
1 agttggggtg gcctcctgct tcctgtggtt ctgtctttct gactgatccc gttgagaaca
61 catatgtatg gaacccgagc gtcaagtttc acatccagtc acggtccaca tctccttcca
121 catctagtga agctgagcca gttaagactg tagactccac tccacttcca gttcacacac
181 caaccttaag gaaaaaagga tgtcctggtt caactggctt tccacttaag cgcaagactc
241 accagttttt tgtaaaatct tttactactc ctaccaagtg tcatcagtgt acctccttga
301 tggtgggttt aataagacag ggctgttcat gtgaagtatg tggattctca tgccatataa
361 cttgtgtaaa caaagctcca accacttgtc cagttcctcc tgaacagaca aaaggtcccc
421 tgggtataga tcctcagaaa ggaataggaa cagcatatga aggtcattca ggattcctaa
481 accagccgga gtgaagaaag ggtggcagag agcactggct atagtgtgtg acttcaaact
541 gtttctgtac gatattgctg aaggaaaagc atctcagccc agtgttgtca ttagtcaagt
601 gattgacatg agggatgaag aattttctgt gagttcagtg ttggcttctg atgttatcca
661 tgcaagtcga aaagatatac cctgtatatt tagggtcaca gcttcccagc tctcagcatc
721 taataacaaa tgttcaatcc tgatgctagc agacagtgag aatgagaaga gtaagtgggt
781 gggagtgctg agtgaattgc acaagatttt gaagaaaaac aaattcagag accgctcagt
841 ctatgttccc aaagaggctt atgacagcac tctacccctc attaaaacaa cccaggcagc
901 cgcaatcata gatcatgaaa gaattgcttt gggaaacgaa gaagggttat ttgttgtaca
961 tgtcaccaaa gatgaaatta ttcgagttgg tgataataag aagattcatc agattgaact
1021 cattccaaat gatcagcttg ttgctgtgat ctcaggacga aatcgtcatg tacgactttt
1081 tcctctgtca gcactggatg ggcgagagac cgatttttac aagctggcag aaactaaagg
1141 gtgtcaaacc ataacttctg gaaaggtgcg ccatggagct ctcacatgcc tgtgtgtggc
1201 tatgaaaagg caggtcctct gttatgaact atttcagagc aagactcgtc acagaaaatt
1261 taaggaaatt caagtcccat ataatgtcca gtggatggca atcttcagtg aacaactctg
1321 tgtgggattc cagtcaggat ttctaagata ccctttgaat ggagaaggaa gtccatacag
1381 tatgctccat tcaaatgacc atacactatc atttattgca catcaaccaa tggatgctat
1441 ctgcgcagtt gagatctcca gtaaagaata tctgctgtgt tttaacagca ttgggatata
1501 tactgactgc cagggccgaa gatctagaca acaggaattg atgtggccag caaatccttc
1561 ctcttgttgt tacaatgcac catatctctc ggtgtacagt gaaaatgcag ttgatatctt
1621 tgatgtgaac tccatggaat ggattcagac tcttcctctc aaaaaggttc gacccttaaa
1681 caatgaagga tcattaaatc ttttagggct ggagaccatt agattaatat atttcaaaaa
1741 taagatggca gaaggggacg aactggtagt tcctgaaaca tcagataata gtcggaaaca
1801 aatggttaga aatattaaca ataagcggcg ttattccttc agagtcccag aagaggaaag
1861 gatgcagcag aggagggaaa tgctacgaga tccagaaatg agaaataaat taatttctaa
1921 tccaactaat tttaaccaca tagcacacat gggtcctgga gatggaatac agatccttaa
1981 agatctgccc atgaaccctc ggcctcagga aagtcggaca gtattcagtg gctcagtcag
2041 tattccatct atcaccaaat cccgccctga gccaggccgc tccatgagtg ctagcagtgg
2101 cctgtcagca aatggttgag ttcaagtctt ctgatgcaga ttgagtttcc accattcatt
2161 tgctcttcat tcagcaagtc tgattttggc acccaatttg tgcccagggt catccgcaca
2221 acaaagacag actttccata gcagcttaga ttaacgttga tttcattcca tgcacttaga
2281 gttgctttca gtaacatttt gcccctactc ccaaaggtag cttaaataga ttacacaaat
2341 gtaagtgata agaataagat tagacagatt ttgctttcac agtagagtct cattatagtc
2401 ctaaaatagc tcatgggatt ctccgcatcc agaagggaga atccgtccct ggagtggctc
2461 actaagctct taatcggcaa ccacagtgag tgtcaacctg attgttgcca ggaaatcctt
2521 atgaattaaa acaatgcata ttttactaca aaaaaaaaaa aaaaaaaaaa aa
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