LOCUS AB179125 2208 bp mRNA linear PRI 06-OCT-2006
DEFINITION Macaca fascicularis testis cDNA clone: QtsA-13209, similar to
human epoxide hydrolase 1, microsomal (xenobiotic) (EPHX1), mRNA,
RefSeq: NM_000120.2.
ACCESSION AB179125
VERSION AB179125.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Macaca fascicularis (crab-eating macaque)
ORGANISM Macaca fascicularis
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Cercopithecidae; Cercopithecinae; Macaca.
REFERENCE 1 (bases 1 to 2208)
AUTHORS Hashimoto,K., Kusuda,J. and Sugano,S.
TITLE Direct Submission
JOURNAL Submitted (10-MAY-2004) to the DDBJ/EMBL/GenBank databases.
Contact:Katsuyuki Hashimoto
National Institute of Infectious Diseases, Division of Genetic
Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan
URL :http://www.nih.go.jp/yoken/genebank/
REFERENCE 2
AUTHORS
CONSRTM International consortium for macaque cDNA sequencing and analysis
TITLE DNA sequences of macaque genes expressed in brain or testis and
its evolutionary implications
JOURNAL Unpublished (2004)
REFERENCE 3
AUTHORS Osada,N., Hirata,M., Tanuma,R., Kusuda,J., Hida,M., Suzuki,Y.,
Sugano,S., Gojobori,T., Shen,C.-K.J., Wu,C.I. and Hashimoto,K.
TITLE Substitution Rate and Structural Divergence of 5'UTR Evolution:
Comparative Analysis Between Human and Cynomolgus Monkey cDNAs
JOURNAL Mol. Biol. Evol. 22, 1976-1982 (2005)
COMMENT The International consortium for macaque cDNA sequencing
and analysis consists of:Department of Virology and Human
Genome Center, Institute of Medical Science, The University of
Tokyo,
Tokyo, Japan; Division of Genetic Resources, National Institute
of Infectious Diseases of Japan, Tokyo, Japan; National Health
Research Institute, Taipei, Taiwan; Institute of Molecular Biology,
Academia Sinica, Taipei, Taiwan; Department of Ecology &
Evolution,
University of Chicago, Chicago, IL, USA, Center for Information
Biology,
National Institute of Genetics of Japan, Mishima, Japan.
Clone distribution: clone distribution information can be
found at: http://www.nih.go.jp/yoken/genebank/
Lab host: TOP10
Vector: pME18S-FL3 (Acc.No. AB009864)
R. Site1: DraIII (CACTGTGTG)
R. Site2: DraIII (CACCATGTG)
Description: 1st strand cDNA was primed with an oligo(dT) primer
[ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was
synthesized using specific 5' and 3' primers and amplified
by PCR. The PCR product was digested with SfiI and size selection
was performed to exclude fragments <1.5kb.The SfiI-digested
PCR product was cloned into distinct DraIII sites of pME18S-FL3.
XhoI sites just outside the DraIII sites can be used to
isolate the cDNA insert. Libraries were constructed by
oligo-capping method. Libraries were made from:
QccE: cerebellum cortex
QnpA: parietal lobe
QtrA: temporal lobe right
QflA: frontal lobe left
QmoA: medulla oblongata
QbsA: brain stem
QorA: occipital lobe right
QtsA: testis
Custom primers were used for 5' and 3'-end sequencing. The
full-insert
sequencing was done by primer-walking method using ABI DNA
sequencer.
FEATURES Location/Qualifiers
source 1..2208
/clone="QtsA-13209"
/clone_lib="macaque cDNA library QtsA"
/db_xref="taxon:9541"
/dev_stage="adult"
/mol_type="mRNA"
/organism="Macaca fascicularis"
/sex="male"
CDS 502..1950
/note="Homo sapiens epoxide hydrolase 1, microsomal
(xenobiotic) (EPHX1), mRNA, RefSeq: NM_000120.2"
/protein_id="BAE02176.1"
/translation="MWLEILLTSVLGFAIYWFISRDKEETLPLEDGWWGPGTRPTGRE
DESIHPFKVETSDEEIHDLHQRIDKFRFTPPLEDSCFHYGFNSNYLKKVISYWRNEFN
WKKQVEILNRYPHFKTKIEGLDIHFIHVKPPQLPAGRTPKPLLMVHGWPGSFYEFYKI
IPLLTDPKNHGLSDEHVFEVICPSIPGYGFSEASSKKGFNSVATARIFYKLMLRLGFQ
EFYIQGGDWGALICTNMAQLVPSHVKGLHLNMALVLSNFSTLTLLLGRRFGRFLGYTE
RDVELLYPVKEKVFYSLMRESGYMHIQCTKPDTVGSALNDSPVGLAAYILEKFSTWTN
TEFRYLEDGGLERKFSLDDLLTNVMLYWTTATIVSSQRFYKENLGQGWMTQKHERMKV
YVPTGFSAFPSELMHTPEKWVRFKYPKLISYSYMARGGHFAALRNQSCSPRTSASSCQ
CWSSSDHPTPPPASLHKCPPGFSLGRYPFSEE"
BASE COUNT 585 a 611 c 543 g 469 t
ORIGIN
1 acttttccac ttcaggccac gatgccgcag taccagacct gggaggagtt cagccgcgct
61 gccgaaaaac tttacctcgc tgatcctatg aagagttcag ggcgactgtg accctgaagc
121 accagtgacc gagggcacct gctgctgccg ccaggagatg tacattgacc tgcaggggat
181 aaagtgggct gaaaactggg tactggagcc cccggggttc ctggcttatg agtgtgtggg
241 cacctgccag cagcccccgg aggccctggc cttcaagtgg ccgtttctgg ggcctggaca
301 gtgcatcgcc tgggagactg ccttgctgcc catgagattc acttacaaag ttacgggatc
361 aaactgctgt acaagctgct aaacgcttct ctccatttct gtcatcgggg ccgcggaccg
421 ccctttgagt agcctgtttt acctcctgcg gaggtggagc cctgaggagg tgtagagact
481 ttcccggatc tctcaggagc catgtggcta gaaatcctcc tgacttcagt gctgggcttt
541 gccatctact ggttcatctc ccgggacaaa gaggaaactt tgccacttga agatgggtgg
601 tggggcccag gcacgaggcc cacaggcagg gaagacgaga gcatccaccc tttcaaggtg
661 gaaacgtcag atgaggagat ccacgactta caccagagga tcgataagtt ccgtttcacc
721 ccacctttgg aggacagctg cttccactat ggcttcaact ccaactacct gaagaaagtc
781 atctcctact ggcggaatga attcaactgg aagaagcagg tggagattct caatagatac
841 cctcacttca agactaagat tgaagggctg gacatccact tcatccatgt gaagcccccc
901 cagctgcccg caggccgtac cccgaagccc ttgctgatgg tgcatggctg gcctggctct
961 ttctacgagt tttataagat catcccactc ctgactgacc ccaagaacca tggcctgagc
1021 gatgagcacg tttttgaagt catctgccct tccatccccg gctatggctt ttcagaggca
1081 tcctccaaga aggggttcaa ctcggtggcc accgccagga tcttctacaa gctgatgctg
1141 cggctgggct tccaggaatt ctacattcaa ggaggggact ggggggccct gatctgcacc
1201 aatatggccc agctggtgcc cagccacgtg aaaggcctgc acttgaacat ggctttggtt
1261 ttaagcaact tctctaccct gacccttctc ctgggacggc gtttcgggag gtttcttggc
1321 tacactgaga gagatgtgga gctgctgtac cctgtcaagg agaaggtctt ctacagcctg
1381 atgagggaga gcggctacat gcacatccag tgcaccaagc ccgacaccgt gggctctgct
1441 ctgaatgact ctcctgtggg tctggctgcc tatattctag agaagttttc cacatggacc
1501 aatacggaat tccgatacct ggaggatgga ggcctggaaa ggaagttctc cctggatgac
1561 ctgctgacca acgtcatgct ctactggaca acagcaacca tcgtctcctc ccagcgcttc
1621 tacaaggaga acctgggaca gggctggatg acccagaagc atgagcggat gaaggtctac
1681 gtgcccactg gcttctctgc cttcccttct gagctaatgc acacgcctga aaagtgggtg
1741 aggttcaagt acccgaagct catctcctat tcctacatgg ctcgtggggg ccactttgca
1801 gccttgagga accagagctg ctcgcccagg acatccgcaa gttcctgtca gtgctggagc
1861 agcagtgacc accccacacc gccccctgcc tccctccaca agtgccctcc aggcttttct
1921 ttgggaagat accccttttc tgaggaatga gtttgcctcc gtcccctgcc catgctggga
1981 gcccacactt accccctcac ccctccaagc tcactcccca atccccaact ctgtgcgata
2041 agcaatatgg ccttgatgat aaatgacttt acttctaaaa aaaaaaaaaa aaaaaaaaaa
2101 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
2161 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaa
//