LOCUS AB171235 1759 bp mRNA linear PRI 06-MAR-2007
DEFINITION Macaca fascicularis brain cDNA clone: QorA-13574, similar to human
prosaposin (variant Gaucher disease and variantmetachromatic
leukodystrophy) (PSAP), mRNA, RefSeq: NM_002778.1.
ACCESSION AB171235
VERSION AB171235.1
KEYWORDS oligo capping; fis (full insert sequence).
SOURCE Macaca fascicularis (crab-eating macaque)
ORGANISM Macaca fascicularis
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Cercopithecidae; Cercopithecinae; Macaca.
REFERENCE 1 (bases 1 to 1759)
AUTHORS Chien,H.-C., Wang,H.-Y., Liu,Y.-L., Wu,K.-M., Ho,T.-C., Tsai,S.-F.
and Shen,C.-K.J.
TITLE Direct Submission
JOURNAL Submitted (19-MAR-2004) to the DDBJ/EMBL/GenBank databases.
Contact:Che-Kun James Shen
Institute of Molecular Biology, Academia Sinica (IMB, AC); 128,
Section 2, Academy Road, Taipei 115, Taiwan
URL :http://www.imb.sinica.edu.tw
REFERENCE 2
AUTHORS Wang,H.-Y., Chien,H.-C., Osada,N., Hashimoto,K., Sugano,S.,
Gojobori,T., Chou,C.-K., Tsai,S.-F., Wu,C.-I. and Shen,C.-K.J.
TITLE Rate of Evolution in Brain-Expressed Genes in Humans and Other
Primates
JOURNAL PLoS Biol. 5, e13 (2007)
COMMENT The International consortium for macaque cDNA sequencing
and analysis consists of: Department of Virology and Human
Genome Center, Institute of Medical Science, The University of
Tokyo, Tokyo, Japan; Division of Genetic Resources, National
Institute of Infectious Diseases of Japan, Tokyo, Japan; National
Health Research Institute, Taipei, Taiwan; Institute of Molecular
Biology, Academia Sinica, Taipei, Taiwan; Department of Ecology &
Evolution, University of Chicago, Chicago, IL, USA; Center for
Information Biology, National Institute of Genetics of Japan,
Mishima, Japan.
Clone distribution: clone distribution information can be
found at: http://www.nih.go.jp/yoken/genebank/
Lab host: TOP10
Vector: pME18S-FL3 (Acc.No. AB009864)
R. Site1: DraIII (CACTGTGTG)
R. Site2: DraIII (CACCATGTG)
Description: 1st strand cDNA was primed with an oligo(dT) primer
[ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was
synthesized using specific 5' and 3' primers and amplified
by PCR. The PCR product was digested with SfiI and size selection
was performed to exclude fragments <1.5kb.The SfiI-digested
PCR product was cloned into distinct DraIII sites of pME18S-FL3.
XhoI sites just outside the DraIII sites can be used to
isolate the cDNA insert. Libraries were constructed by
oligo-capping method. Libraries were made from:
QccE: cerebellum cortex
QnpA: parietal lobe
QtrA: temporal lobe right
QflA: frontal lobe left
QmoA: medulla oblongata
QbsA: brain stem
QorA: occipital lobe right
QtsA: testis
Custom primers were used for 5' and 3'-end sequencing. The
full-insert sequencing was done by primer-walking method using ABI
DNA sequencer.
FEATURES Location/Qualifiers
source 1..1759
/clone="QorA-13574"
/clone_lib="macaque cDNA library QorA"
/db_xref="taxon:9541"
/dev_stage="adult"
/mol_type="mRNA"
/organism="Macaca fascicularis"
/sex="male"
CDS 609..1445
/note="Homo sapiens prosaposin (variant Gaucher disease
and variantmetachromatic leukodystrophy) (PSAP), mRNA,
RefSeq: NM_002778.1"
/protein_id="BAE88298.1"
/translation="MGDVCQDCIQMVTDIQTAVRTNSTFVQALVEHVKEECDRLGPGM
ADMCKNYISQYSEIAIQMMMHMQDQQPKEICALVGFCDEVKEMPMQTLVPAKVASKNV
IPALELVEPIKKHEVPAKSDVYCEVCEFLVKEVTKLIDNNKTEKEILDTFDKMCSKLP
KSLSEECQEVVDTYGSSILSILLQEVSPELVCSMLRLCSGTRLPALTVHMTQPKDGGF
CEVCKKLVGYLDRNLEKNSTKQEILACVSVWTVVAALCLLVLPTCPWRRCCCSLHPSP
VS"
BASE COUNT 413 a 447 c 504 g 395 t
ORIGIN
1 attgcagact gcggagtcag gcggcgctat gtacgctctc ttcctcctgg ccagcctcct
61 gggcgcggct ctagccagcc cagtcctcgg aatgaaagaa tgcaccagag gctcggcagt
121 gtggtgccag aatgtgaaga cggcatccga ctgcggggca gtgaagcact gcctgcagac
181 cgtttggaac aagccgacag tgaaatccct tccttgtgac atatgcaaag atgttgtcac
241 cgcagctggt gatatgctga aggacaatgc caccgaggag gagatccttg tttacttgga
301 gaagacctgt gactggcttc cgaaaccgaa catgtctgct tcgtgcaagg agatagtgga
361 ctcctacctc cctgtcatcc tggacatcat taaaggagaa atgagccgtc ctggggaggt
421 gtgctctgct ctcaacctct gcgagtctct ccagaagcac ctggcagagc tgaatcacca
481 gaaacagctg gagtccaata agatcccaga gctggacatg actgaggtgg tggccccctt
541 catggccaac atccctctcc tcctctaccc tcaggacagc ccccgcagca agccccagcc
601 aaaggataat gggggacgtt tgccaggact gcattcagat ggtgaccgac atccagactg
661 ctgtacggac caactccacc tttgtccagg ccttggtgga acatgtcaag gaggagtgtg
721 accgcctggg ccctggcatg gccgacatgt gcaagaacta tatcagccag tattctgaaa
781 ttgctatcca gatgatgatg cacatgcagg atcagcaacc caaggagatc tgtgcgctgg
841 ttgggttctg tgatgaggtg aaagagatgc ccatgcagac tctggtcccc gccaaagtgg
901 cctccaagaa tgtcatccct gccctggaac tggtggagcc cattaagaag cacgaggtcc
961 cagcaaagtc tgatgtttac tgtgaggtgt gtgaattcct ggtgaaggag gtgaccaagc
1021 tgattgacaa caacaagact gagaaagaaa tactcgacac ttttgacaaa atgtgctcga
1081 agctgccgaa gtccctgtcg gaagagtgcc aggaggtggt ggacacgtac ggcagctcaa
1141 tcctgtccat cctgctgcag gaggtcagcc ctgagctggt gtgcagcatg ctgcgcctct
1201 gctctggcac gcggctgcct gcgctgaccg ttcatatgac tcagccgaag gacggtggct
1261 tctgcgaagt gtgcaagaag ctggtgggtt atttggatcg taacctggag aaaaacagca
1321 ccaagcagga gatcctggct tgtgtgagcg tgtggacagt ggtggccgcg ctgtgcctgc
1381 tcgtgttgcc tacgtgtccc tggaggaggt gctgctgcag cctgcacccc tcccccgtct
1441 catagaggct ccttttgacc tttcttttca aataaatgtg gatggcgagt tcctaggcct
1501 gtggctcctg ttagagggcg gcatgctgag gggtctgctg ggtgtgggtt ggacgctggg
1561 gtgtgggttg gatgctgtct gcagcccact tgggcaccca tgcttctgtc tacttctggt
1621 tgccaggaga cagcaagcaa agccagcagg atacgcagtt gctattaaat ggactttgtg
1681 agttttgttt tgcactaaag tttctgtgat ttaacaataa aattctgtta gccacaaaaa
1741 aaaaaaaaaa aaaaaaaaa
//