LOCUS       AB170870                1760 bp    mRNA    linear   PRI 06-MAR-2007
DEFINITION  Macaca fascicularis brain cDNA clone: QorA-10690, similar to human
            prosaposin (variant Gaucher disease and variantmetachromatic
            leukodystrophy) (PSAP), mRNA, RefSeq: NM_002778.1.
ACCESSION   AB170870
VERSION     AB170870.1
KEYWORDS    oligo capping; fis (full insert sequence).
SOURCE      Macaca fascicularis (crab-eating macaque)
  ORGANISM  Macaca fascicularis
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Cercopithecidae; Cercopithecinae; Macaca.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Chien,H.-C., Wang,H.-Y., Liu,Y.-L., Wu,K.-M., Ho,T.-C., Tsai,S.-F.
            and Shen,C.-K.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2004) to the DDBJ/EMBL/GenBank databases.
            Contact:Che-Kun James Shen
            Institute of Molecular Biology, Academia Sinica (IMB, AC); 128,
            Section 2, Academy Road, Taipei 115, Taiwan
            URL    :http://www.imb.sinica.edu.tw
REFERENCE   2
  AUTHORS   Wang,H.-Y., Chien,H.-C., Osada,N., Hashimoto,K., Sugano,S.,
            Gojobori,T., Chou,C.-K., Tsai,S.-F., Wu,C.-I. and Shen,C.-K.J.
  TITLE     Rate of Evolution in Brain-Expressed Genes in Humans and Other
            Primates
  JOURNAL   PLoS Biol. 5, e13 (2007)
COMMENT     The International consortium for macaque cDNA sequencing
            and analysis consists of: Department of Virology and Human
            Genome Center, Institute of Medical Science, The University of
            Tokyo, Tokyo, Japan; Division of Genetic Resources, National
            Institute of Infectious Diseases of Japan, Tokyo, Japan; National
            Health Research Institute, Taipei, Taiwan; Institute of Molecular
            Biology, Academia Sinica, Taipei, Taiwan; Department of Ecology &
            Evolution, University of Chicago, Chicago, IL, USA; Center for
            Information Biology, National Institute of Genetics of Japan,
            Mishima, Japan.
            Clone distribution: clone distribution information can be
            found at: http://www.nih.go.jp/yoken/genebank/
            
            Lab host:    TOP10
            Vector:      pME18S-FL3 (Acc.No. AB009864)
            R.  Site1:   DraIII (CACTGTGTG)
            R.  Site2:   DraIII (CACCATGTG)
            Description: 1st strand cDNA was primed with an oligo(dT) primer
            [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was
            synthesized using specific 5' and 3' primers and amplified
            by PCR.  The PCR product was digested with SfiI and size selection
            was performed to exclude fragments <1.5kb.The SfiI-digested
            PCR product was cloned into distinct DraIII sites of pME18S-FL3.
            XhoI sites just outside the DraIII sites can be used to
            isolate the cDNA insert.  Libraries were constructed by
            oligo-capping method. Libraries were made from:
                         QccE: cerebellum cortex
                         QnpA: parietal lobe
                         QtrA: temporal lobe right
                         QflA: frontal lobe left
                         QmoA: medulla oblongata
                         QbsA: brain stem
                         QorA: occipital lobe right
                         QtsA: testis
            Custom primers were used for 5' and 3'-end sequencing. The
            full-insert sequencing was done by primer-walking method using ABI
            DNA sequencer.
FEATURES             Location/Qualifiers
     source          1..1760
                     /clone="QorA-10690"
                     /clone_lib="macaque cDNA library QorA"
                     /db_xref="taxon:9541"
                     /dev_stage="adult"
                     /mol_type="mRNA"
                     /organism="Macaca fascicularis"
                     /sex="male"
     CDS             31..1677
                     /note="Homo sapiens prosaposin (variant Gaucher disease
                     and variantmetachromatic leukodystrophy) (PSAP), mRNA,
                     RefSeq: NM_002778.1"
                     /protein_id="BAE87933.1"
                     /translation="MYALFLLASLLGAALASPVLGMKECTRGSAVWCQNVKTASDCGA
                     VKHCLQTVWNKPTVKSLPCDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNM
                     SASCKEIVDSYLPVILDIIKGEMSRPGEVCSALNLCESLQKHLAELNHQKQLESNKIP
                     ELDMTEVVAPFMANIPLLLYPQDSPRSKPQPKDNGDVCQDCIQMVTDIQTAVRTNSTF
                     VQALVEHVKEECDRLGPGMADMCKNYISQYSEIAIQMMMHMQDQQPKEICALVGFCDE
                     VKEMPMQTLVPAKVASKNVIPALELVEPIKKHEVPAKSDVYCEVCEFLVKEVTKLIDN
                     NKTEKEILDTFDKMCSKLPKSLSEECQEVVDTYGSSILSILLQEVSPELVCSMLRLCS
                     GTRLPALTVHMTQPKDGGFCEVCKKLVGYLDRNLEKNSTKQEILAALEKGCSFLPDPY
                     QKQCDQFVAEYEPVLIEILIEAPFDLSFQINVDGEFLGLWLLLEGGMLRGLLGVGWTL
                     GCGLDAVCSPLGHPCFCLLLVARRQQAKPAGYAVAIKWTL"
BASE COUNT          430 a          442 c          496 g          392 t
ORIGIN      
        1 gcattgcaga ctgcggagtc aggcggcgct atgtacgctc tcttcctcct ggccagcctc
       61 ctgggcgcgg ctctagccag cccagtcctc ggaatgaaag aatgcaccag aggctcggca
      121 gtgtggtgcc agaatgtgaa gacggcatcc gactgcgggg cagtgaagca ctgcctgcag
      181 accgtttgga acaagccgac agtgaaatcc cttccttgtg acatatgcaa agatgttgtc
      241 accgcagctg gtgatatgct gaaggacaat gccaccgagg aggagatcct tgtttacttg
      301 gagaagacct gtgactggct tccgaaaccg aacatgtctg cttcgtgcaa ggagatagtg
      361 gactcctacc tccctgtcat cctggacatc attaaaggag aaatgagccg tcctggggag
      421 gtgtgctctg ctctcaacct ctgcgagtct ctccagaagc acctggcaga gctgaatcac
      481 cagaaacagc tggagtccaa taagatccca gagctggaca tgactgaggt ggtggccccc
      541 ttcatggcca acatccctct cctcctctac cctcaggaca gcccccgcag caagccccag
      601 ccaaaggata atggggacgt ttgccaggac tgcattcaga tggtgaccga catccagact
      661 gctgtacgga ccaactccac ctttgtccag gccttggtgg aacatgtcaa ggaggagtgt
      721 gaccgcctgg gccctggcat ggccgacatg tgcaagaact atatcagcca gtattctgaa
      781 attgctatcc agatgatgat gcacatgcag gatcagcaac ccaaggagat ctgtgcgctg
      841 gttgggttct gtgatgaggt gaaagagatg cccatgcaga ctctggtccc cgccaaagtg
      901 gcctccaaga atgtcatccc tgccctggaa ctggtggagc ccattaagaa gcacgaggtc
      961 ccagcaaagt ctgatgttta ctgtgaggtg tgtgaattcc tggtgaagga ggtgaccaag
     1021 ctgattgaca acaacaagac tgagaaagaa atactcgaca cttttgacaa aatgtgctcg
     1081 aagctgccga agtccctgtc ggaagagtgc caggaggtgg tggacacgta cggcagctca
     1141 atcctgtcca tcctgctgca ggaggtcagc cctgagctgg tgtgcagcat gctgcgcctc
     1201 tgctctggca cgcggctgcc tgcgctgacc gttcatatga ctcagccgaa ggacggtggc
     1261 ttctgcgaag tgtgcaagaa gctggtgggt tatttggatc gtaacctgga gaaaaacagc
     1321 accaagcagg agatcctggc tgctcttgag aaaggctgca gcttcctgcc cgacccttac
     1381 cagaagcagt gtgatcagtt tgtggcagag tacgagcccg tgctgatcga gatcctcata
     1441 gaggctcctt ttgacctttc ttttcaaata aatgtggatg gcgagttcct aggcctgtgg
     1501 ctcctgttag agggcggcat gctgaggggt ctgctgggtg tgggttggac gctggggtgt
     1561 gggttggatg ctgtctgcag cccacttggg cacccatgct tctgtctact tctggttgcc
     1621 aggagacagc aagcaaagcc agcaggatac gcagttgcta ttaaatggac tttgtgagtt
     1681 ttgttttgca ctaaagtttc tgtgatttaa caataaaatt ctgttagcca caaaaaaaaa
     1741 aaaaaaaaaa aaaaaaaaaa
//