LOCUS AB170870 1760 bp mRNA linear PRI 06-MAR-2007 DEFINITION Macaca fascicularis brain cDNA clone: QorA-10690, similar to human prosaposin (variant Gaucher disease and variantmetachromatic leukodystrophy) (PSAP), mRNA, RefSeq: NM_002778.1. ACCESSION AB170870 VERSION AB170870.1 KEYWORDS oligo capping; fis (full insert sequence). SOURCE Macaca fascicularis (crab-eating macaque) ORGANISM Macaca fascicularis Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Cercopithecidae; Cercopithecinae; Macaca. REFERENCE 1 (bases 1 to 1760) AUTHORS Chien,H.-C., Wang,H.-Y., Liu,Y.-L., Wu,K.-M., Ho,T.-C., Tsai,S.-F. and Shen,C.-K.J. TITLE Direct Submission JOURNAL Submitted (19-MAR-2004) to the DDBJ/EMBL/GenBank databases. Contact:Che-Kun James Shen Institute of Molecular Biology, Academia Sinica (IMB, AC); 128, Section 2, Academy Road, Taipei 115, Taiwan URL :http://www.imb.sinica.edu.tw REFERENCE 2 AUTHORS Wang,H.-Y., Chien,H.-C., Osada,N., Hashimoto,K., Sugano,S., Gojobori,T., Chou,C.-K., Tsai,S.-F., Wu,C.-I. and Shen,C.-K.J. TITLE Rate of Evolution in Brain-Expressed Genes in Humans and Other Primates JOURNAL PLoS Biol. 5, e13 (2007) COMMENT The International consortium for macaque cDNA sequencing and analysis consists of: Department of Virology and Human Genome Center, Institute of Medical Science, The University of Tokyo, Tokyo, Japan; Division of Genetic Resources, National Institute of Infectious Diseases of Japan, Tokyo, Japan; National Health Research Institute, Taipei, Taiwan; Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan; Department of Ecology & Evolution, University of Chicago, Chicago, IL, USA; Center for Information Biology, National Institute of Genetics of Japan, Mishima, Japan. Clone distribution: clone distribution information can be found at: http://www.nih.go.jp/yoken/genebank/ Lab host: TOP10 Vector: pME18S-FL3 (Acc.No. AB009864) R. Site1: DraIII (CACTGTGTG) R. Site2: DraIII (CACCATGTG) Description: 1st strand cDNA was primed with an oligo(dT) primer [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was synthesized using specific 5' and 3' primers and amplified by PCR. The PCR product was digested with SfiI and size selection was performed to exclude fragments <1.5kb.The SfiI-digested PCR product was cloned into distinct DraIII sites of pME18S-FL3. XhoI sites just outside the DraIII sites can be used to isolate the cDNA insert. Libraries were constructed by oligo-capping method. Libraries were made from: QccE: cerebellum cortex QnpA: parietal lobe QtrA: temporal lobe right QflA: frontal lobe left QmoA: medulla oblongata QbsA: brain stem QorA: occipital lobe right QtsA: testis Custom primers were used for 5' and 3'-end sequencing. The full-insert sequencing was done by primer-walking method using ABI DNA sequencer. FEATURES Location/Qualifiers source 1..1760 /clone="QorA-10690" /clone_lib="macaque cDNA library QorA" /db_xref="taxon:9541" /dev_stage="adult" /mol_type="mRNA" /organism="Macaca fascicularis" /sex="male" CDS 31..1677 /note="Homo sapiens prosaposin (variant Gaucher disease and variantmetachromatic leukodystrophy) (PSAP), mRNA, RefSeq: NM_002778.1" /protein_id="BAE87933.1" /translation="MYALFLLASLLGAALASPVLGMKECTRGSAVWCQNVKTASDCGA VKHCLQTVWNKPTVKSLPCDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNM SASCKEIVDSYLPVILDIIKGEMSRPGEVCSALNLCESLQKHLAELNHQKQLESNKIP ELDMTEVVAPFMANIPLLLYPQDSPRSKPQPKDNGDVCQDCIQMVTDIQTAVRTNSTF VQALVEHVKEECDRLGPGMADMCKNYISQYSEIAIQMMMHMQDQQPKEICALVGFCDE VKEMPMQTLVPAKVASKNVIPALELVEPIKKHEVPAKSDVYCEVCEFLVKEVTKLIDN NKTEKEILDTFDKMCSKLPKSLSEECQEVVDTYGSSILSILLQEVSPELVCSMLRLCS GTRLPALTVHMTQPKDGGFCEVCKKLVGYLDRNLEKNSTKQEILAALEKGCSFLPDPY QKQCDQFVAEYEPVLIEILIEAPFDLSFQINVDGEFLGLWLLLEGGMLRGLLGVGWTL GCGLDAVCSPLGHPCFCLLLVARRQQAKPAGYAVAIKWTL" BASE COUNT 430 a 442 c 496 g 392 t ORIGIN 1 gcattgcaga ctgcggagtc aggcggcgct atgtacgctc tcttcctcct ggccagcctc 61 ctgggcgcgg ctctagccag cccagtcctc ggaatgaaag aatgcaccag aggctcggca 121 gtgtggtgcc agaatgtgaa gacggcatcc gactgcgggg cagtgaagca ctgcctgcag 181 accgtttgga acaagccgac agtgaaatcc cttccttgtg acatatgcaa agatgttgtc 241 accgcagctg gtgatatgct gaaggacaat gccaccgagg aggagatcct tgtttacttg 301 gagaagacct gtgactggct tccgaaaccg aacatgtctg cttcgtgcaa ggagatagtg 361 gactcctacc tccctgtcat cctggacatc attaaaggag aaatgagccg tcctggggag 421 gtgtgctctg ctctcaacct ctgcgagtct ctccagaagc acctggcaga gctgaatcac 481 cagaaacagc tggagtccaa taagatccca gagctggaca tgactgaggt ggtggccccc 541 ttcatggcca acatccctct cctcctctac cctcaggaca gcccccgcag caagccccag 601 ccaaaggata atggggacgt ttgccaggac tgcattcaga tggtgaccga catccagact 661 gctgtacgga ccaactccac ctttgtccag gccttggtgg aacatgtcaa ggaggagtgt 721 gaccgcctgg gccctggcat ggccgacatg tgcaagaact atatcagcca gtattctgaa 781 attgctatcc agatgatgat gcacatgcag gatcagcaac ccaaggagat ctgtgcgctg 841 gttgggttct gtgatgaggt gaaagagatg cccatgcaga ctctggtccc cgccaaagtg 901 gcctccaaga atgtcatccc tgccctggaa ctggtggagc ccattaagaa gcacgaggtc 961 ccagcaaagt ctgatgttta ctgtgaggtg tgtgaattcc tggtgaagga ggtgaccaag 1021 ctgattgaca acaacaagac tgagaaagaa atactcgaca cttttgacaa aatgtgctcg 1081 aagctgccga agtccctgtc ggaagagtgc caggaggtgg tggacacgta cggcagctca 1141 atcctgtcca tcctgctgca ggaggtcagc cctgagctgg tgtgcagcat gctgcgcctc 1201 tgctctggca cgcggctgcc tgcgctgacc gttcatatga ctcagccgaa ggacggtggc 1261 ttctgcgaag tgtgcaagaa gctggtgggt tatttggatc gtaacctgga gaaaaacagc 1321 accaagcagg agatcctggc tgctcttgag aaaggctgca gcttcctgcc cgacccttac 1381 cagaagcagt gtgatcagtt tgtggcagag tacgagcccg tgctgatcga gatcctcata 1441 gaggctcctt ttgacctttc ttttcaaata aatgtggatg gcgagttcct aggcctgtgg 1501 ctcctgttag agggcggcat gctgaggggt ctgctgggtg tgggttggac gctggggtgt 1561 gggttggatg ctgtctgcag cccacttggg cacccatgct tctgtctact tctggttgcc 1621 aggagacagc aagcaaagcc agcaggatac gcagttgcta ttaaatggac tttgtgagtt 1681 ttgttttgca ctaaagtttc tgtgatttaa caataaaatt ctgttagcca caaaaaaaaa 1741 aaaaaaaaaa aaaaaaaaaa //