LOCUS AB170090 1827 bp mRNA linear PRI 06-MAR-2007
DEFINITION Macaca fascicularis brain cDNA clone: QbsB-10150, similar to human
prosaposin (variant Gaucher disease and variantmetachromatic
leukodystrophy) (PSAP), mRNA, RefSeq: NM_002778.1.
ACCESSION AB170090
VERSION AB170090.1
KEYWORDS oligo capping; fis (full insert sequence).
SOURCE Macaca fascicularis (crab-eating macaque)
ORGANISM Macaca fascicularis
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Cercopithecidae; Cercopithecinae; Macaca.
REFERENCE 1 (bases 1 to 1827)
AUTHORS Chien,H.-C., Wang,H.-Y., Liu,Y.-L., Wu,K.-M., Ho,T.-C., Tsai,S.-F.
and Shen,C.-K.J.
TITLE Direct Submission
JOURNAL Submitted (19-MAR-2004) to the DDBJ/EMBL/GenBank databases.
Contact:Che-Kun James Shen
Institute of Molecular Biology, Academia Sinica (IMB, AC); 128,
Section 2, Academy Road, Taipei 115, Taiwan
URL :http://www.imb.sinica.edu.tw
REFERENCE 2
AUTHORS Wang,H.-Y., Chien,H.-C., Osada,N., Hashimoto,K., Sugano,S.,
Gojobori,T., Chou,C.-K., Tsai,S.-F., Wu,C.-I. and Shen,C.-K.J.
TITLE Rate of Evolution in Brain-Expressed Genes in Humans and Other
Primates
JOURNAL PLoS Biol. 5, e13 (2007)
COMMENT The International consortium for macaque cDNA sequencing
and analysis consists of: Department of Virology and Human
Genome Center, Institute of Medical Science, The University of
Tokyo, Tokyo, Japan; Division of Genetic Resources, National
Institute of Infectious Diseases of Japan, Tokyo, Japan; National
Health Research Institute, Taipei, Taiwan; Institute of Molecular
Biology, Academia Sinica, Taipei, Taiwan; Department of Ecology &
Evolution, University of Chicago, Chicago, IL, USA; Center for
Information Biology, National Institute of Genetics of Japan,
Mishima, Japan.
Clone distribution: clone distribution information can be
found at: http://www.nih.go.jp/yoken/genebank/
Lab host: TOP10
Vector: pME18S-FL3 (Acc.No. AB009864)
R. Site1: DraIII (CACTGTGTG)
R. Site2: DraIII (CACCATGTG)
Description: 1st strand cDNA was primed with an oligo(dT) primer
[ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was
synthesized using specific 5' and 3' primers and amplified
by PCR. The PCR product was digested with SfiI and size selection
was performed to exclude fragments <1.5kb.The SfiI-digested
PCR product was cloned into distinct DraIII sites of pME18S-FL3.
XhoI sites just outside the DraIII sites can be used to
isolate the cDNA insert. Libraries were constructed by
oligo-capping method. Libraries were made from:
QccE: cerebellum cortex
QnpA: parietal lobe
QtrA: temporal lobe right
QflA: frontal lobe left
QmoA: medulla oblongata
QbsA: brain stem
QorA: occipital lobe right
QtsA: testis
Custom primers were used for 5' and 3'-end sequencing. The
full-insert sequencing was done by primer-walking method using ABI
DNA sequencer.
FEATURES Location/Qualifiers
source 1..1827
/clone="QbsB-10150"
/clone_lib="macaque cDNA library QbsB"
/db_xref="taxon:9541"
/dev_stage="adult"
/mol_type="mRNA"
/organism="Macaca fascicularis"
/sex="male"
CDS 29..949
/note="Homo sapiens prosaposin (variant Gaucher disease
and variantmetachromatic leukodystrophy) (PSAP), mRNA,
RefSeq: NM_002778.1"
/protein_id="BAE87153.1"
/translation="MYALFLLASLLGAALASPVLGMKECTRGSAVWCQNVKTASDCGA
VKHCLQTVWNKPTVKSLPCDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNM
SASCKEIVDSYLPVILDIIKGEMSRPGEVCSALNLCESLQKHLAELNHQKQLESNKIP
ELVCSMLRLCSGTRLPALTVHMTQPKDGGFCEVCKKLVGYLDRNLEKNSTKQEILAAL
EKGCSFLPDPYQKQCDQFVAEYEPVLIEILVEVMDPSFVCLKIGACPSAHKPLLGTEK
CVWGPSYWCQNTETAAQCNAVEHCKRHVWN"
BASE COUNT 373 a 470 c 536 g 448 t
ORIGIN
1 attgcagact gcggagtcag gcggcgctat gtacgctctc ttcctcctgg ccagcctcct
61 gggcgcggct ctagccagcc cagtcctcgg aatgaaagaa tgcaccagag gctcggcagt
121 gtggtgccag aatgtgaaga cggcatccga ctgcggggca gtgaagcact gcctgcagac
181 cgtttggaac aagccgacag tgaaatccct tccttgtgac atatgcaaag atgttgtcac
241 cgcagctggt gatatgctga aggacaatgc caccgaggag gagatccttg tttacttgga
301 gaagacctgt gactggcttc cgaaaccgaa catgtctgct tcgtgcaagg agatagtgga
361 ctcctacctc cctgtcatcc tggacatcat taaaggagaa atgagccgtc ctggggaggt
421 gtgctctgct ctcaacctct gcgagtctct ccagaagcac ctggcagagc tgaatcacca
481 gaaacagctg gagtccaata agatccctga gctggtgtgc agcatgctgc gcctctgctc
541 tggcacgcgg ctgcctgcgc tgaccgttca tatgactcag ccgaaggacg gtggcttctg
601 cgaagtgtgc aagaagctgg tgggttattt ggatcgtaac ctggagaaaa acagcaccaa
661 gcaggagatc ctggctgctc ttgagaaagg ctgcagcttc ctgcccgacc cttaccagaa
721 gcagtgtgat cagtttgtgg cagagtacga gcccgtgctg atcgagatcc tggtggaggt
781 gatggatcct tcgttcgtgt gcttgaaaat tggagcctgc ccgtcagccc ataagccctt
841 gctgggaact gagaagtgtg tatggggccc aagctactgg tgccagaaca cagagacggc
901 agcccagtgc aatgctgtcg agcattgcaa acgccacgtg tggaactagg aggaggaata
961 ttccagcttg gagaaactgc agcattagtt tttttctact tgtgtgtctg ggagaatgaa
1021 cgcacagatc tatttgactt tgttataaaa atagggctcc ctcacctccc ccatttctgt
1081 gtcctttgtt gtagcattgc tgtctgcaag ggagccccta gcccctggca gacatagctg
1141 cttcagtggc ccttttctct ctgctagatg gatgttgatg cactggaggt cttttagccc
1201 gccctcgcat ggcacctgct ggaggaggag agagctctgc tggcatgagc cacagcttct
1261 tgactggagg ccatcaaccc tcttggctgg ggccttgttc tgagccctga catgtgcttg
1321 ggcactggtg ggcctgggct tctgaggtgg cctcctgccc tggtcaggga ccctctcccc
1381 tttcctgggc ctctcagctg tacaaagcag caaaacaaag gcagttttat atgaaagatt
1441 agaagcccgg aataatcagg ctttttaaat gatttaattc ccgctgtaat agcacaggga
1501 ttttggaagc agctgctggt ggcttgtgtg agcgtgtgga cagtggtggc cgcgctgtgc
1561 ctgctcgtgt tgcctacgtg tccctggctg ctgaggtgct gctgcagcct gcacccctcc
1621 cccgtctcat agaggctcct tttgaccttt cttttcaaat aaatgtggat ggcgagttcc
1681 taggcctgtg gctcctgtta gagggcggca tgctgagggg tctgctgggt gtgggttgga
1741 cgctggggtg tgggttggat gctgtctgca gcccacttgg gcacccatgc ttctgtctac
1801 ttctggttgc caggagacag caagcaa
//