LOCUS AB169654 2637 bp mRNA linear PRI 06-OCT-2006
DEFINITION Macaca fascicularis brain cDNA, clone: QccE-13090, similar to
human prosaposin (variant Gaucher disease and variantmetachromatic
leukodystrophy) (PSAP), mRNA, RefSeq: NM_002778.1.
ACCESSION AB169654
VERSION AB169654.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Macaca fascicularis (crab-eating macaque)
ORGANISM Macaca fascicularis
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Cercopithecidae; Cercopithecinae; Macaca.
REFERENCE 1 (bases 1 to 2637)
AUTHORS Hashimoto,K., Kusuda,J. and Sugano,S.
TITLE Direct Submission
JOURNAL Submitted (18-MAR-2004) to the DDBJ/EMBL/GenBank databases.
Contact:Katsuyuki Hashimoto
National Institute of Infectious Diseases, Division of Genetic
Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan
URL :http://www.nih.go.jp/yoken/genebank/
REFERENCE 2
AUTHORS
CONSRTM International consortium for macaque cDNA sequencing and analysis
TITLE DNA sequences of macaque genes expressed in brain or testis and
its evolutionary implications
JOURNAL Unpublished (2004)
REFERENCE 3
AUTHORS Osada,N., Hirata,M., Tanuma,R., Kusuda,J., Hida,M., Suzuki,Y.,
Sugano,S., Gojobori,T., Shen,C.-K.J., Wu,C.I. and Hashimoto,K.
TITLE Substitution Rate and Structural Divergence of 5'UTR Evolution:
Comparative Analysis Between Human and Cynomolgus Monkey cDNAs
JOURNAL Mol. Biol. Evol. 22, 1976-1982 (2005)
COMMENT The International consortium for macaque cDNA sequencing
and analysis consists of:Department of Virology and Human
Genome Center, Institute of Medical Science, The University of
Tokyo, Tokyo, Japan; Division of Genetic Resources, National
Institute of Infectious Diseases of Japan, Tokyo, Japan; National
Health Research Institute, Taipei, Taiwan; Institute of Molecular
Biology, Academia Sinica, Taipei, Taiwan; Department of Ecology &
Evolution, University of Chicago, Chicago, IL, USA, Center for
Information Biology, National Institute of Genetics of Japan,
Mishima, Japan.
Clone distribution: clone distribution information can be
found at: http://www.nih.go.jp/yoken/genebank/
Lab host: TOP10
Vector: pME18S-FL3 (Acc.No. AB009864)
R. Site1: DraIII (CACTGTGTG)
R. Site2: DraIII (CACCATGTG)
Description: 1st strand cDNA was primed with an oligo(dT) primer
[ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was
synthesized using specific 5' and 3' primers and amplified
by PCR. The PCR product was digested with SfiI and size selection
was performed to exclude fragments <1.5kb.The SfiI-digested
PCR product was cloned into distinct DraIII sites of pME18S-FL3.
XhoI sites just outside the DraIII sites can be used to
isolate the cDNA insert. Libraries were constructed by
oligo-capping method. Libraries were made from:
QccE: cerebellum cortex
QnpA: parietal lobe
QtrA: temporal lobe right
QflA: frontal lobe left
QmoA: medulla oblongata
QbsA: brain stem
QorA: occipital lobe right
QtsA: testis
Custom primers were used for 5' and 3'-end sequencing. The
full-insert sequencing was done by primer-walking method using ABI
DNA sequencer.
FEATURES Location/Qualifiers
source 1..2637
/clone="QccE-13090"
/clone_lib="macaque cDNA library QccE"
/db_xref="taxon:9541"
/dev_stage="adult"
/mol_type="mRNA"
/organism="Macaca fascicularis"
/sex="male"
CDS 73..1566
/note="Homo sapiens prosaposin (variant Gaucher disease
and variantmetachromatic leukodystrophy) (PSAP), mRNA,
RefSeq: NM_002778.1"
/protein_id="BAE01735.1"
/translation="MYALFLLASLLGAALASPVLGMKECTRGSAVWCQNVKTASDCGV
VKHCLQTVWNKPTVKSLPCDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNM
SASCKEIVDSYLPVILDIIKGEMSRPGEVCSALNLCESLQKHLAELNHQKQLESNKIP
ELDMTEVVAPFMANIPLLLYPQDGPRSKPQPKDNGDVCQDCIQMVTDIQTAVRTNSTF
VQALVEHVKEECDRLGPGMADMCKNYISQYSEIAIQMMMHMQDQQPKEICALVGFCDE
VKEMPMQTLVPAKVASKNVIPALELVEPIKKHEVPAKSDVYCEVCEFLVKEVTKLIDN
NKTEKEILDTFDKMCSKLPKSLSEECQEVVDTYGSSILSILLQEVSPELVCSMLRLCS
GTRLPALTVHVTQPKDGGFCEVCKKLVGYLDRNLEKNSTKQEILAALEKGCSFLPDPY
QKQCDQFVAEYEPVLIEILVEVMDPSFVFFSTCVSGRMNAQIYLTLL"
BASE COUNT 573 a 669 c 754 g 641 t
ORIGIN
1 aaaaagggca gatttatatc tgcggggatc agctgacgct ccgcattgca gactgcggag
61 tcaggcggcg ctatgtacgc tctcttcctc ctggccagcc tcctgggcgc ggctctagcc
121 agcccagtcc tcggaatgaa agaatgcacc agaggctcgg cagtgtggtg ccagaatgtg
181 aagacggcat ccgactgcgg ggtagtgaag cactgcctgc agaccgtttg gaacaagccg
241 acagtgaaat ctcttccttg tgacatatgc aaagacgttg tcaccgcagc tggtgatatg
301 ctgaaggaca atgccaccga ggaggagatc cttgtttact tggagaagac ctgtgactgg
361 cttccgaaac cgaacatgtc tgcttcgtgc aaggagatag tggactccta cctccctgtc
421 atcctggaca tcattaaagg agaaatgagc cgtcctgggg aggtgtgctc tgctctcaac
481 ctctgcgagt ctctccagaa gcacctggca gagctgaatc accagaagca gctggagtcc
541 aataagatcc cagagctgga catgactgag gtggtggccc ccttcatggc caacatccct
601 ctcctcctct accctcagga cggcccccgc agcaagcccc agccaaagga taatggggac
661 gtttgccagg actgcattca gatggtgacc gacatccaga ctgctgtacg gaccaactcc
721 acctttgtcc aggccttggt ggaacatgtc aaggaggagt gtgaccgcct gggccctggc
781 atggccgaca tgtgcaagaa ctatatcagc cagtattctg aaattgctat ccagatgatg
841 atgcacatgc aggatcagca acccaaggag atctgtgcgc tggttgggtt ctgtgatgag
901 gtgaaagaga tgcccatgca gactctggtc cccgccaaag tggcctccaa gaatgtcatc
961 cctgccctgg aactggtgga gcccattaag aagcacgagg tcccagcaaa gtctgatgtt
1021 tactgtgagg tgtgtgaatt cctggtgaag gaggtgacca agctgattga caacaacaag
1081 actgagaaag aaatactcga cacttttgac aaaatgtgct cgaagctgcc gaagtccctg
1141 tcggaagagt gccaggaggt ggtggacacg tacggcagct caatcctgtc catcctgctg
1201 caggaggtca gccctgagct ggtgtgcagc atgctgcgcc tctgctctgg cacgcggctg
1261 cctgcgctga ccgttcatgt gactcagcca aaggacggtg gcttctgcga agtgtgcaag
1321 aagctggtgg gttatttgga tcgtaacctg gagaaaaaca gcaccaagca ggagatcctg
1381 gctgctcttg agaaaggctg cagcttcctg cccgaccctt accagaagca gtgtgatcag
1441 tttgtggcag agtacgagcc cgtgctgatc gagatcctgg tggaggtgat ggatccttcg
1501 ttcgtgtttt tttctacttg tgtgtctggg agaatgaacg cacagatcta tttgactttg
1561 ttataaaaat agggctccct cacctccccc atttctgtgt cctttgttgt agcattgctg
1621 tctgcaaggg agcccctagc ccctggcaga catagctgct tcagtggccc ttttctctct
1681 gctagatgga tgttgatgca ctggaggtct tttagcccgc cctcgcatgg cacctgctgg
1741 aggaggagag agctctgctg gcatgagcca cagcttcttg actggaggcc atcaaccctc
1801 ttggctgggg ccttgttctg agccctgaca tgtgcttggg cactggtggg cctgggcttc
1861 tgaggtggcc tcctgccctg gtcagggacc ctcgcccctt tcctgggcct ctcagctgta
1921 caaagcagca aaacaaaggc agttttatat gaaagattag aagcccggaa taatcaggct
1981 ttttaaatga tttaattccc gctgtaatag cacagggatt ttggaagcag ctgctggtgg
2041 cttgggaccc cagtggggcc acggtttctc tgtccctggt tcaactgtga tttggctttc
2101 ccgtgtcttt cctggtgatg ccttgtttgg ggttctgtgg gtttgggtgg gaagggggcc
2161 atctgcctga atgtaacctg ctagctctcc aaaggccccg cgggcctggc ttgtgtgagc
2221 gtgtggacag tggtggccgc gctgtgcctg ctcgtgttac ctacgtgtcc ctggctgctg
2281 aggcgctgct gcagcctgca cccctccccc gtctcataga ggctcctttt gacctttctt
2341 ttcaaataaa tgtggatggc gagttcctag gcctgtggct cctgttagag ggcggcatgc
2401 tgaggggtct gctgggtgtg ggttggacgc tggggtgtgg gttggatgct gtctgcagcc
2461 cacttgggca cccatgcttc tgtctacttc tggttgccag gagacagcaa gcaaagccag
2521 caggatacgc agttgctatt aaatggactt tgtgagtttt gttttgcact aaagtttctg
2581 tgatttaaca ataaaattct gttagccaaa aaaaaaaaaa aaaaaaaaaa aaaaaaa
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