LOCUS AB169197 1470 bp mRNA linear PRI 06-OCT-2006
DEFINITION Macaca fascicularis testis cDNA, clone: QtsA-17935, similar to
human signal recognition particle 9kDa (SRP9), mRNA, RefSeq:
NM_003133.1.
ACCESSION AB169197
VERSION AB169197.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Macaca fascicularis (crab-eating macaque)
ORGANISM Macaca fascicularis
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Cercopithecidae; Cercopithecinae; Macaca.
REFERENCE 1 (bases 1 to 1470)
AUTHORS Hashimoto,K., Kusuda,J. and Sugano,S.
TITLE Direct Submission
JOURNAL Submitted (18-MAR-2004) to the DDBJ/EMBL/GenBank databases.
Contact:Katsuyuki Hashimoto
National Institute of Infectious Diseases, Division of Genetic
Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan
URL :http://www.nih.go.jp/yoken/genebank/
REFERENCE 2
AUTHORS
CONSRTM International consortium for macaque cDNA sequencing and analysis
TITLE DNA sequences of macaque genes expressed in brain or testis and
its evolutionary implications
JOURNAL Unpublished (2004)
REFERENCE 3
AUTHORS Osada,N., Hirata,M., Tanuma,R., Kusuda,J., Hida,M., Suzuki,Y.,
Sugano,S., Gojobori,T., Shen,C.-K.J., Wu,C.I. and Hashimoto,K.
TITLE Substitution Rate and Structural Divergence of 5'UTR Evolution:
Comparative Analysis Between Human and Cynomolgus Monkey cDNAs
JOURNAL Mol. Biol. Evol. 22, 1976-1982 (2005)
COMMENT The International consortium for macaque cDNA sequencing
and analysis consists of: Department of Virology and Human
Genome Center, Institute of Medical Science, The University of
Tokyo, Tokyo, Japan; Division of Genetic Resources, National
Institute of Infectious Diseases of Japan, Tokyo, Japan; National
Health Research Institute, Taipei, Taiwan; Institute of Molecular
Biology, Academia Sinica, Taipei, Taiwan; Department of Ecology &
Evolution, University of Chicago, Chicago, IL, USA; Center for
Information Biology, National Institute of Genetics of Japan,
Mishima, Japan.
Clone distribution: clone distribution information can be
found at: http://www.nih.go.jp/yoken/genebank/
Lab host: TOP10
Vector: pME18S-FL3 (Acc.No. AB009864)
R. Site1: DraIII (CACTGTGTG)
R. Site2: DraIII (CACCATGTG)
Description: 1st strand cDNA was primed with an oligo(dT) primer
[ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was
synthesized using specific 5' and 3' primers and amplified
by PCR. The PCR product was digested with SfiI and size selection
was performed to exclude fragments <1.5kb.The SfiI-digested
PCR product was cloned into distinct DraIII sites of pME18S-FL3.
XhoI sites just outside the DraIII sites can be used to
isolate the cDNA insert. Libraries were constructed by
oligo-capping method. Libraries were made from:
QccE: cerebellum cortex
QnpA: parietal lobe
QtrA: temporal lobe right
QflA: frontal lobe left
QmoA: medulla oblongata
QbsA: brain stem
QorA: occipital lobe right
QtsA: testis
Custom primers were used for 5' and 3'-end sequencing. The
full-insert sequencing was done by primer-walking method using ABI
DNA sequencer.
FEATURES Location/Qualifiers
source 1..1470
/clone="QtsA-17935"
/clone_lib="macaque cDNA library QtsA"
/db_xref="taxon:9541"
/dev_stage="adult"
/mol_type="mRNA"
/organism="Macaca fascicularis"
/sex="male"
CDS 97..357
/note="Homo sapiens signal recognition particle 9kDa
(SRP9), mRNA, RefSeq: NM_003133.1"
/protein_id="BAE01289.1"
/translation="MPQYQTWEEFSRAAEKLYLADPMKARVVLKYRHSDGNLCVKVTD
DLVCLVYKTEQAQDVKKIEKFHSQLMRLMVAKESRNVTMETE"
BASE COUNT 469 a 220 c 290 g 491 t
ORIGIN
1 tgggactcgc gtcggttggc gactcccgga cgtaggtagt ttgttgggcc gggttctgag
61 gcgttggttc tctttacttt tccacttcag gccacgatgc cgcagtacca gacctgggag
121 gagttcagcc gcgctgccga aaaactttac ctcgctgatc ctatgaaggc acgtgtggtt
181 ctcaaatata ggcattctga tgggaacttg tgtgttaaag taacagatga tttagtttgt
241 ttggtgtata aaacagagca agctcaagat gtaaagaaga ttgagaaatt ccacagtcaa
301 ctaatgcgac ttatggtagc caaggaatcc cgcaatgtta ccatggaaac tgagtgactg
361 gtttgaaatg aagactttgt catgtactta ggaagtaaat atcttgaaat agagaaagtg
421 ttgggacaga aagtacttta tgtaactaag tggactattc agaagcttag aggtcatttt
481 ttgtaatttt ctttttaatg tttacattag agagctaggg atgcaaatgt tttcagttag
541 aaagccttta tttacttttg gaaattgaac aagaaatgca tctgtcttgg aaactttgca
601 gattatttga tgttagataa aacatgtaat tgtttctctg gcaaatttat atcagtaatt
661 tgaaaatgag atattagaaa aaaccaattc ttaaatttag ttcatcttta aaagaacatt
721 aaatataacc attttgttgg atcgatgtgt ttcttttgga gcataaaatg tatgctgttg
781 tgaccaataa atataaaata tggaaattgg aattaactcc acaccatagt atgcattgtt
841 atgcatacgg tgcacctaat tatgtatacc agtgtagtct caattatatc taaaagtagt
901 tgtgactaac aagtatgctt tgccttattt ccacatttaa actacctttt aacgtaaggg
961 atttgtagta tcagcttgtt gagcaatgac tttgaatcga gttttcagtg atcagaagca
1021 gcagttactt gagtgcatga atggaatgat aactataatg tagtgaaacc accatattac
1081 agaaatattt actacatatt ttccatctgt agtttttcag agaggctatg gattagttga
1141 actgtcaaat cttgcatact tctgtgacac ccttgcctgt tttctctaac caaggtgtta
1201 ggtgtgactg tcacaactgt taatgttttc cggtaaacta gaagtataat attatttggt
1261 aattatattt gtaccctaat tttcaccacc taaatgtaat gttgattccc aagaatgaaa
1321 tgaaggcact acattgaaat atgttttgta taaatttgtc atgttgaaca acattttagc
1381 atggtaagtt cccttagcta tatgaatttt ggcatgtttc agagagatca gtaaataaaa
1441 tattacataa aataaaaaaa aaaaaaaaaa
//