LOCUS AB168419 2469 bp mRNA linear PRI 06-OCT-2006
DEFINITION Macaca fascicularis testis cDNA clone: QtsA-12009, similar to
human TUDOR gene similar (TGS), mRNA, RefSeq: NM_173083.2.
ACCESSION AB168419
VERSION AB168419.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Macaca fascicularis (crab-eating macaque)
ORGANISM Macaca fascicularis
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Cercopithecidae; Cercopithecinae; Macaca.
REFERENCE 1 (bases 1 to 2469)
AUTHORS Hashimoto,K., Kusuda,J. and Sugano,S.
TITLE Direct Submission
JOURNAL Submitted (18-MAR-2004) to the DDBJ/EMBL/GenBank databases.
Contact:Katsuyuki Hashimoto
National Institute of Infectious Diseases, Division of Genetic
Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan
URL :http://www.nih.go.jp/yoken/genebank/
REFERENCE 2
AUTHORS
CONSRTM International consortium for macaque cDNA sequencing and analysis
TITLE DNA sequences of macaque genes expressed in brain or testis and
its evolutionary implications
JOURNAL Unpublished (2004)
REFERENCE 3
AUTHORS Osada,N., Hirata,M., Tanuma,R., Kusuda,J., Hida,M., Suzuki,Y.,
Sugano,S., Gojobori,T., Shen,C.-K.J., Wu,C.I. and Hashimoto,K.
TITLE Substitution Rate and Structural Divergence of 5'UTR Evolution:
Comparative Analysis Between Human and Cynomolgus Monkey cDNAs
JOURNAL Mol. Biol. Evol. 22, 1976-1982 (2005)
COMMENT The International consortium for macaque cDNA sequencing
and analysis consists of: Department of Virology and Human
Genome Center, Institute of Medical Science, The University of
Tokyo, Tokyo, Japan; Division of Genetic Resources, National
Institute of Infectious Diseases of Japan, Tokyo, Japan; National
Health Research Institute, Taipei, Taiwan; Institute of Molecular
Biology, Academia Sinica, Taipei, Taiwan; Department of Ecology &
Evolution, University of Chicago, Chicago, IL, USA; Center for
Information Biology, National Institute of Genetics of Japan,
Mishima, Japan.
Clone distribution: clone distribution information can be
found at: http://www.nih.go.jp/yoken/genebank/
Lab host: TOP10
Vector: pME18S-FL3 (Acc.No. AB009864)
R. Site1: DraIII (CACTGTGTG)
R. Site2: DraIII (CACCATGTG)
Description: 1st strand cDNA was primed with an oligo(dT) primer
[ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was
synthesized using specific 5' and 3' primers and amplified
by PCR. The PCR product was digested with SfiI and size selection
was performed to exclude fragments <1.5kb.The SfiI-digested
PCR product was cloned into distinct DraIII sites of pME18S-FL3.
XhoI sites just outside the DraIII sites can be used to
isolate the cDNA insert. Libraries were constructed by
oligo-capping method. Libraries were made from:
QccE: cerebellum cortex
QnpA: parietal lobe
QtrA: temporal lobe right
QflA: frontal lobe left
QmoA: medulla oblongata
QbsA: brain stem
QorA: occipital lobe right
QtsA: testis
Custom primers were used for 5' and 3'-end sequencing. The
full-insert sequencing was done by primer-walking method using ABI
DNA sequencer.
FEATURES Location/Qualifiers
source 1..2469
/clone="QtsA-12009"
/clone_lib="macaque cDNA library QtsA"
/db_xref="taxon:9541"
/dev_stage="adult"
/mol_type="mRNA"
/organism="Macaca fascicularis"
/sex="male"
CDS 25..1653
/note="Homo sapiens TUDOR gene similar (TGS), mRNA,
RefSeq: NM_173083.2"
/protein_id="BAE00540.1"
/translation="MAELDQLPDESSSAKALVSLKEGSLSNTWNEKYSSLQKTPVWKG
RNTSPAVEMPFRNSKRSRLFSDEDDRQINTRSPKRNQRVAMVPQKFTATMSTPDKKAS
QKIGFRLRNLLKLPKAHKWCIYEWFYSNIDKPLFEGDNDFCVCLKESFPNLKTRKLTR
VEWGKIRRLMGKPRRCSSAFFEEERSALKQKRQKIRLLQQRKVADVSQFKDLPDEIPL
PLVIGTKVTARLRGVHDGLFTGQIDAVDTLNATYRVTFDRTGLGTHTIPDYEVLSNEP
HETMPIAAFGQKQRPSRFFMTPPRLHYTPPLQSPIMDNDPLLGQSPWRSKISGSDTET
LGGFPVEFLIQVTRLSKILMIKKEHIKKLREMNTDAEKLKSYSMPISIEFQRRYATIV
LELEQLNKDLNKVLHKVQQYCYELAPDQGLQPADQPTDMRRRCEEEAQEIVRHANSST
GQPCVENENLTDLISRLTAILLQIKCLAEGGDLNSFEFKSLTDSLNDIKSTIDASNIS
CFQNNVEIHVAHIQSGLSQMGNLHAFAANNTNRD"
BASE COUNT 859 a 438 c 480 g 692 t
ORIGIN
1 aaaaagcggc gcggctcgtt caagatggcg gagctcgacc agttgcctga cgagagttct
61 tcagcaaaag cccttgtcag tttaaaagag ggaagcttat ctaacacatg gaatgaaaag
121 tacagttctt tacagaaaac acctgtttgg aaaggcagga atacaagccc tgctgtggaa
181 atgcctttca gaaattcaaa acgaagtcga cttttttctg atgaagatga taggcaaata
241 aatacaaggt cacctaaaag aaaccagagg gttgcaatgg ttccacagaa atttacagca
301 acaatgtcaa caccagataa gaaagcttca cagaagattg gttttcgatt acgtaatctg
361 ctcaagcttc ctaaagcaca taaatggtgt atatacgagt ggttctattc aaatatagat
421 aaaccgcttt ttgaaggtga taatgacttc tgtgtatgtc taaaggaatc ttttcctaat
481 ttgaaaacaa gaaagttaac cagagtagaa tggggaaaaa ttaggcggct tatgggaaaa
541 ccacggagat gttcttctgc attttttgag gaagagagat cagcattaaa acagaaacgg
601 cagaaaataa ggctcttaca acaaaggaaa gttgcagatg tttcacaatt caaagatctc
661 ccagatgaaa ttcctttgcc tctggttatt ggaacgaaag ttacagcacg attacgtggt
721 gttcatgatg gtttgttcac tggacaaata gatgctgtgg atactcttaa tgctacttat
781 agagtaactt ttgataggac agggcttgga acccatacca tccctgacta tgaagttctc
841 agtaatgagc ctcatgagac aatgccaatt gctgcctttg gacagaaaca gcggccttct
901 cgatttttta tgaccccacc acggttacat tatactcctc ctctccagtc accaattatg
961 gataatgatc ctttattggg acagtcgccg tggagaagta aaatttctgg ctctgacact
1021 gaaacattag gtggttttcc agtagaattt cttatccaag tgacccgatt atcaaaaatt
1081 ctcatgatta aaaaggaaca tatcaagaaa ttaagggaaa tgaacacaga cgcagaaaaa
1141 ctgaaatcat attccatgcc catcagcatt gaatttcagc ggagatatgc aacaattgtt
1201 ctagagcttg aacagctgaa caaggaccta aacaaagttt tgcataaagt tcaacagtat
1261 tgctatgagc ttgctccaga ccaggggctc cagcctgcag atcagccaac agatatgaga
1321 cgcaggtgtg aggaagaagc acaggaaatt gttcggcatg caaattcctc aacaggacag
1381 ccctgtgtgg aaaatgaaaa tctgacagac ttaatttcca ggcttacagc tattttgtta
1441 caaattaagt gtctagcaga aggaggagac ctgaattcct ttgaattcaa atcacttaca
1501 gactcattaa atgatatcaa gagtacaata gatgcttcta atatcagttg cttccagaat
1561 aatgtagaaa tccatgttgc acatattcag agtggcctga gccagatggg aaacttacat
1621 gcctttgcag caaataacac caacagagac tgagtaaaga tttcattatt ccaactgcac
1681 gggacattgt ttttgagaag ttcttttccc ttgtataggc ttccaacacc gaataaccta
1741 accgctggaa aacaagggaa atttaaatct ccaaataagg cattttaata gactgtactg
1801 cttcttaaac cagcattgct gaccagcatt atatttattt ttcttttatt attctgatgc
1861 agtagcattg cttatgttac atatgtttat attcacaaat atttttaaac tgaaatatct
1921 gaacataata taatttcgtg gaagattaca ttgaccattt tttttaatgt gcatgaattc
1981 accgcaacac atgcagacaa ctgctgcaat ggagagtatg aagaaacctg gtctttttgt
2041 tcatgtcggt ggcagtgtgg aaattccatc cagaaaatta caactccact tgatttagtt
2101 gatcaccatc tcagtcttca aaagataaca tcatgaggtg tgggaagtcc tagttttaag
2161 gaagccactg aaatatagat gggaaatgtg gactatacaa gtatatgtta tatatacttg
2221 caatgtgaca tggttctata gatcatttta taataataaa tattttaatt tatcataact
2281 tatataaaag aaacctttgc tgtttgttga aagaaaatga aggaacagga agaaaggtgc
2341 aaaatgctaa atttctgcaa atggatttgg catgtcttcc cgtcagttca ggtcaaaagt
2401 gcattgttat gagatttatt taaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
2461 aaaaaaaaa
//