LOCUS       AB121443                 762 bp    DNA     linear   BCT 03-OCT-2003
DEFINITION  Plasmid pUB110 htk gene for highly thermostable kanamycin
            nucleotidyltransferase, complete cds.
ACCESSION   AB121443
VERSION     AB121443.1
KEYWORDS    .
SOURCE      Plasmid pUB110
  ORGANISM  Plasmid pUB110
            other sequences; plasmids.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Hoseki,J., Yano,T., Koyama,Y., Kuramitsu,S. and Kagamiyama,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-OCT-2003) to the DDBJ/EMBL/GenBank databases.
            Contact:Jun Hoseki
            Osaka Medical College, Biochemistry; 2-7 Daigaku-machi, Takatsuki,
            Osaka 569-8686, Japan
REFERENCE   2
  AUTHORS   Hoseki,J., Yano,T., Koyama,Y., Kuramitsu,S. and Kagamiyama,H.
  TITLE     Directed evolution of thermostable kanamycin-resistance gene: a
            convenient selection marker for Thermus thermophilus
  JOURNAL   J. Biochem. 126, 951-956 (1999)
REFERENCE   3
  AUTHORS   Hashimoto,Y., Yano,T., Kuramitsu,S. and Kagamiyama,H.
  TITLE     Disruption of Thermus thermophilus genes by homologous
            recombination using a thermostable kanamycin-resistant marker
  JOURNAL   FEBS Lett. 506, 231-234 (2001)
COMMENT     
FEATURES             Location/Qualifiers
     source          1..762
                     /db_xref="taxon:2655"
                     /mol_type="genomic DNA"
                     /organism="Plasmid pUB110"
     CDS             1..762
                     /citation=[2]
                     /codon_start=1
                     /EC_number="2.7.7.46"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /gene="htk"
                     /note="a mutant containing 21 amino-acid substitutions"
                     /product="highly thermostable kanamycin
                     nucleotidyltransferase"
                     /protein_id="BAC98411.1"
                     /transl_table=11
                     /translation="MKGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLGRQT
                     DGPYSDIEMMCVLSTEGVEFSYEWTTGEWKAEVNFYSEEILLDYASRVEPDWPLTHGR
                     FFSILPIYDPGGYFEKVYQTAKSVEAQKFHDAICALIVEELFEYAGKWRNIRVQGPTT
                     FLPSLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQPDLPPGYVQLCQLVMSGQLS
                     DPEKLLESLENFWNGVQEWAERHGYIVDVSKRIPF"
BASE COUNT          214 a          136 c          200 g          212 t
ORIGIN      
        1 atgaaaggac caataataat gactagagaa gaaagaatga agattgttca tgaaattaag
       61 gaacgaatat tggataaata tggggatgat gttaaggcaa ttggtgttta tggctctctt
      121 ggtcgtcaga ctgatgggcc ctattcggat attgagatga tgtgtgttct gtcaacagag
      181 ggagtagagt tcagctatga atggacaacc ggtgagtgga aggcggaagt gaatttttat
      241 agcgaagaga ttctactaga ttatgcatct cgggtggaac cggattggcc gcttacacat
      301 ggtcgatttt tctctatttt gccgatttat gatccaggtg gatactttga gaaagtgtac
      361 caaactgcta aatcggtaga agcccaaaag ttccacgatg cgatctgtgc ccttatcgta
      421 gaagagctgt ttgaatatgc aggcaaatgg cgtaatattc gtgtgcaagg accgacaaca
      481 tttctaccat ccttgactgt acaggtggca atggcaggtg ccatgttgat tggtctgcat
      541 catcgcatct gttatacgac gagcgcttcg gtcttaactg aagcagttaa gcaaccagat
      601 cttcctccag gttatgtcca actgtgccag ctcgtaatgt ctggtcaact ttccgaccct
      661 gagaaacttc tggaatcgct agagaatttc tggaatgggg ttcaggagtg ggcggaacga
      721 cacggatata tagtggatgt gtcaaaacgc ataccatttt ga
//