LOCUS AB046005 3060 bp mRNA linear PRI 06-OCT-2006
DEFINITION Macaca fascicularis brain cDNA, clone:QccE-13136, similar to human
thymidine kinase 2 mitochondrial (TK2), mRNA, NM_004614.1.
ACCESSION AB046005
VERSION AB046005.1
KEYWORDS FLI_CDNA; fis (full insert sequence).
SOURCE Macaca fascicularis (crab-eating macaque)
ORGANISM Macaca fascicularis
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Cercopithecidae; Cercopithecinae; Macaca.
REFERENCE 1 (bases 1 to 3060)
AUTHORS Hashimoto,K., Osada,N., Hida,M., Kusuda,J. and Sugano,S.
TITLE Direct Submission
JOURNAL Submitted (14-JUL-2000) to the DDBJ/EMBL/GenBank databases.
Contact:Katsuyuki Hashimoto
National Institute of Infectious Diseases, Division of Genetic
Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan
URL :http://www.nih.go.jp/yoken/genebank/
REFERENCE 2
AUTHORS Osada,N., Hida,M., Kusuda,J., Tanuma,R., Iseki,K., Hirai,M.,
Terao,K., Suzuki,Y., Sugano,S. and Hashimoto,K.
TITLE Isolation of full-length cDNA clones from macaque brain cDNA
libraries.
JOURNAL Unpublished (2001)
COMMENT URL: http://www.nih.go.jp/yoken/genebank/
Lib Name: macaque brain cDNA library QccE
Lab host: TOP10
Vector: pME18S-FL3 (Acc.No. AB009864)
R. Site1: DraIII (CACTGTGTG)
R. Site2: DraIII (CACCATGTG)
Description: 1st strand cDNA was primed with an oligo(dT) primer
[ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was
synthesized using specific 5'and 3'primers and amplified
by PCR. The PCR product was digested with SfiI and size selection
was performed to exclude fragments <1.5kb.The SfiI-digested
PCR product was cloned into distinct DraIII sites of pME18S-FL3.
XhoI sites just outside the DraIII sites can be used to
isolate the cDNA insert. Libraries were constructed by
Sugano et al.(University of Tokyo, Institute of Medical
Science). Custom primer used for sequencing
( 5' end primer [CTTCTGCTCTAAAAGCTGCG];
3' end primer [CGACCTGCAGCTCGAGCACA] ).
FEATURES Location/Qualifiers
source 1..3060
/clone="QccE-13136"
/clone_lib="macaque brain cDNA library QccE"
/db_xref="taxon:9541"
/dev_stage="adult"
/mol_type="mRNA"
/organism="Macaca fascicularis"
CDS 472..1269
/codon_start=1
/note="similar to human thymidine kinase 2 mitochondrial
(TK2), mRNA, NM_004614.1"
/product="unnamed protein product"
/protein_id="BAB01587.1"
/transl_table=1
/translation="MLLRPLRGWAALALRCFEPGSPGSPASGPGSRRVQRGAWPSDKE
REKEKKSVICVEGNIASGKTTCLEFFSNATDIEVLTEPVSKWRNVRGHNPLGLMYQDA
SRWGLTLQTYVQLTMLDRHTCPQVSSVRLMERSIHSARYIFVENLYRSGKMPEVDYVV
LSEWFDWILRNMDVSIDLIVYLRTNPETCYQRLKRRCREEEKVIPLEYLEAIHHLHEE
WLIKGSLFPVAAPVLVIEADHHMERMLQLFEQNRDRILTPENRKLGP"
BASE COUNT 721 a 758 c 867 g 714 t
ORIGIN
1 ctgttgcttc tgaccatttg aacaagttac ctaattctct gaggacaagc tcgaagaatg
61 ggagagacaa tatctatatg cagggttgtt gggaggaata agtgacatga gtgtgtgcca
121 ggagtctgat tacagaaggt gttcaattaa gtaatctgca atcattaatc aacccatcag
181 tcactggtat tatgtgccat ccatcctccc agtgttgcca agttatgggt gcgttctgcc
241 agcgtcctag cagtgggaag gcttctggct gccagcggcg aacctctccc ttggagtatt
301 tctcctctcg ctgagaagat gaaatgtgac cgggtctctt taagggccag gcgccgggat
361 ccaggcggcg cgcaagggct ggacgagcag tagcccgcgc cgactctcac aggaaggaag
421 cccgggcctc tggatccact ctccagggcc tctggatccg ctcgcccagt tatgctgctg
481 cggccgctgc ggggctgggc cgccctggcg ctgcgctgct ttgagccggg aagccccggg
541 agcccggcct caggccccgg gtcgcggagg gtgcagcgcg gggcctggcc ttcggataaa
601 gaacgggaaa aagagaaaaa atccgtgatc tgtgtcgagg gcaatattgc aagtgggaag
661 acgacatgcc tggaattctt ctccaatgcg acagacattg aggtattaac ggagcctgtg
721 tccaagtgga gaaatgtccg tggccacaat cctctgggcc tgatgtacca agatgcctct
781 cgctggggcc ttacgctaca gacttacgtg cagctcacca tgctggacag gcatacttgt
841 cctcaggtgt catctgtacg gttgatggag aggtcgattc acagcgcaag atacattttt
901 gtagaaaacc tgtatagaag tgggaagatg ccagaagtgg actatgtagt tctgtcggaa
961 tggtttgact ggatcttgag gaacatggac gtgtccattg atttgattgt ttatcttcgg
1021 accaatcctg agacttgtta ccagaggtta aagaggagat gcagggaaga ggagaaggtc
1081 attccactgg aatacctgga agcaattcac catctccatg aggagtggct catcaaaggc
1141 agccttttcc ccgtggcagc ccctgttctg gtgatcgagg ctgaccacca catggagagg
1201 atgttacaac tctttgaaca aaatcgggat cgaatattaa ctccagagaa tcggaagctt
1261 ggcccatagg aggcagaagg tctatggatc atgtctgaaa aatgcctgct gctgccaagt
1321 tagctattat gagcaatctg gaaaaacttg ctcccaggag ggctttgtgt ctggccggct
1381 tgatttttct aatggtctca tctcctttgc tagtgtcttt gtcataagtc tctggccctc
1441 gtgggtaagt gacaaacggg accaatgggt ttgccaagcc ctttgctgtt cgcagcctca
1501 cattcccccg ctgcctcttc cagtggcttc gtgctgccaa gttgctttca agaagccctt
1561 agggagagca cctgctgtgt gcctggcgtc ccgctggagc tgtgtaccaa tcatctcagc
1621 cttcatcagg aggctgaggt aggagtctta tgtcccaggt gaggaatttg aagctcagaa
1681 agattgaggg ctctccagag gtcacacagc cagtgtgcag tggagctggc accattcaga
1741 ctttcagcca actccacaac tttcctttgc cctggctgcc tcctcctgag agctgttccc
1801 cactgccctg cctcttctgg ttggaggctc tcgtgtctct ttggggagag ctggcagtgt
1861 gtggagccaa taacattttc tcaatgttga gcagttccca aggacagtca gcatttctag
1921 acttccacaa aattgtgctg catttgactg gagcctggtg ttcagtggtt tccccgcccg
1981 aggccactgc aggcccatcc accacgtcct cctgtggatg ttgacgttag gagattcaca
2041 cgctggctcc tgaatggtgc tctgtctcct tggcgatgaa ggtcctgctt cagctgctgc
2101 caactccatg tcagggaagt cgcttttggt gtgtggctgg tttgcccaga gccaagcgga
2161 ggcaaggggc agccagccct ggcttccaag gctcccgtac tatctgtgtc cttctataag
2221 gagctttact cttggaatta ctgaaagtct gtggcccaag ggagagatac aagtggcctt
2281 aagtcttttt gaaatgttct ttcatccagg ggaatgcctc aagccataga gcctgaaatc
2341 atctttgttg gttccgaaaa taccttaact tcactcagct ggaactgcgt tgaaagcaag
2401 gctgtccctt ggatcaagca gaaaacaaga gaaagaaaga atattccctt tggggacagt
2461 ctggaaaact gggattcgca aataaaggct ctggaagcat tgctagtcct gaagcttcgg
2521 aggtgggcag agcgagcttc aagaagactg gatggaaacc ctgggaagga ttaaggctca
2581 actctggaga aacaggccat gggctctcag agcagccgtt agctgtaaat agaggtagca
2641 aggccactcc caggcccctg tgagtatggg cacctgtaca tgcagtgctc cgactctgca
2701 gaggtgccaa gtgaccctgc tgggccagtc ccagagagtt aagaagtcaa ggccggccgg
2761 gcgcggtggc tcaagcctgt aatcccagca ctttgggagg ccgagacggg cggatcacga
2821 ggtcaggaga tcgagaccat cctggctaat atggtgaaac cccgtctcta ctaaaaatac
2881 aaaaaactag ccgggcgagg tggtgggcgc ctgtagtccc agctactcgg gaggctgagg
2941 caggagaatg gcgtaaaccc gggaggcgga gcttgcagtg agctgagatc cggccactgc
3001 actccagcct gggcgacaaa gcgagactcc gtctcaaaaa aaaaaaaaaa aaaaaaaaaa
//