LOCUS AB028069 2780 bp mRNA linear HUM 03-JUN-1999
DEFINITION Homo sapiens ASK/H37 (huDbf4) mRNA for activator of S phase
Kinase, complete cds.
ACCESSION AB028069
VERSION AB028069.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2780)
AUTHORS Masai,H. and Kumagai,H.
TITLE Direct Submission
JOURNAL Submitted (28-MAY-1999) to the DDBJ/EMBL/GenBank databases.
Contact:Hisao Masai
Institute of Medical Science, University of Tokyo, Department of
Developmental Biology; 4-6-1 Shirokanedai, Minato-ku, Tokyo
108-8639, Japan
REFERENCE 2
AUTHORS Kumagai,H., Sato,N., Yamada,M., Mahony,D., Seghezzi,W., Lees,E.,
Arai,K. and Masai,H.
TITLE A Novel Growth- and Cell Cycle-Regulated Protein, ASK, Activates
Human Cdc7-Related Kinase and Is Essential for G1/S transition in
Mammalian Cells
JOURNAL Mol. Cell. Biol. 19 (1999) In press
COMMENT
FEATURES Location/Qualifiers
source 1..2780
/cell_type="HeLa"
/chromosome="7"
/clone="H37"
/db_xref="H-InvDB:HIT000058919"
/db_xref="taxon:9606"
/map="7q21"
/mol_type="mRNA"
/organism="Homo sapiens"
CDS 518..2542
/codon_start=1
/function="binds and activates huCdc7 kinase"
/gene="ASK/H37 (huDbf4)"
/note="ASK encodes a regulatory subunit for huCdc7, the
human homologue of budding yeast Cdc7 kinase."
/note="Amino acid 51-90 and 290-322 are moif-N and
motif-C, respectively, conserved in Dbf4-related
molecules. Amino acid 201-218 is a putative bipartite
nuclear localization signal. Amino acid 101-120 and
552-564 are two putative PEST-like sequences."
/product="activator of S phase Kinase"
/protein_id="BAA78326.1"
/transl_table=1
/translation="MNSGAMRIHSKGHFQGGIQVKNEKNRPSLKSLKTDNRPEKSKCK
PLWGKVFYLDLPSVTISEKLQKDIKDLGGRVEEFLSKDISYLISNKKEAKFAQTLGRI
SPVPSPESAYTAETTSPHPSHDGSSFKSPDTVCLSRGKLLVEKAIKDHDFIPSNSILS
NALSWGVKILHIDDIRYYIEQKKKELYLLKKSSTSVRDGGKRVGSGAQKTRTGRLKKP
FVKVEDMSQLYRPFYLQLTNMPFINYSIQKPCSPFDVDKPSSMQKQTQVKLRIQTDGD
KYGGTSIQLQLKEKKKKGYCECCLQKYEDLETHLLSEQHRNFAQSNQYQVVDDIVSKL
VFDFVEYEKDTPKKKRIKYSVGSLSPVSASVLKKTEQKEKVELQHISQKDCQEDDTTV
KEQNFLYKETQETEKKLLFISEPIPHPSNELRGLNEKMSNKCSMLSTAEDDIRQNFTQ
LPLHKNKQECILDISEHTLSENDLEELRVDHYKCNIQASVHVSDFSTDNSGSQPKQKS
DTVLFPAKDLKEKDLHSIFTHDSGLITINSSQEHLTVQAKAPFHTPPEEPNECDFKNM
DSLPSGKIHRKVKIILGRNRKENLEPNAEFDKRTEFITQEENRICSSPVQSLLDLFQT
SEEKSEFLGFTSYTEKSGICNVLDIWEEENSDNLLTAFFSSPSTSTFTGF"
regulatory 2724..2730
/regulatory_class="polyA_signal_sequence"
BASE COUNT 949 a 534 c 613 g 684 t
ORIGIN
1 aattcggcac gagctctctg aggctgcgcc aagacctgaa gcggcggacc gagagcccgg
61 gtctgagact gagagagcaa cggaatggag gcggggtaga ggcggaaaca caacctgcag
121 ggccagagcg aggcgcgaga aggacggcgg cgtgaggggg cggggcgcgc agcgcgagaa
181 ggcaggcacg aggggcgagc gcgaggcggg gcacggcgcg tggcgtgaga cggggcgggg
241 cgcgcgtatc ggcgccgcgg ccgcgtgacg cgttttcaaa tcttcaaccg ccgcagccca
301 ctcgtttgtg ctttgcgcct tcctcctccg cgccttggag ccggatccgg ccccggaaac
361 ccgacctgca gacgcggtac ctctactgcg tagaggccgt agctggcgga aggagagagg
421 cggccgtcct gtcaacaggc cgggggaagc cgtgctttcg cggctgcccg gtgcgacact
481 ttctccggac ccagcatgta ggtgccgggc gactgccatg aactccggag ccatgaggat
541 ccacagtaaa ggacatttcc agggtggaat ccaagtcaaa aatgaaaaaa acagaccatc
601 tctgaaatct ctgaaaactg ataacaggcc agaaaaatcc aaatgtaagc cactttgggg
661 aaaagtattt taccttgact taccttctgt caccatatct gaaaaacttc aaaaggacat
721 taaggatctg ggagggcgag ttgaagaatt tctcagcaaa gatatcagtt atcttatttc
781 aaataagaag gaagctaaat ttgcacaaac cttgggtcga atttctcctg taccaagtcc
841 agaatctgca tatactgcag aaaccacttc acctcatccc agccatgatg gaagttcatt
901 taagtcacca gacacagtgt gtttaagcag aggaaaatta ttagttgaaa aagctatcaa
961 ggaccatgat tttattcctt caaatagtat attatcaaat gccttgtcat ggggagtaaa
1021 aattcttcat attgatgaca ttagatacta cattgaacaa aagaaaaaag agttgtattt
1081 actcaagaaa tcaagtactt cagtaagaga tgggggcaaa agagttggta gtggtgcaca
1141 aaaaacaaga acaggaagac tcaaaaagcc ttttgtaaag gtggaagata tgagccaact
1201 ttataggcca ttttatcttc agctgaccaa tatgcctttt ataaattatt ctattcagaa
1261 gccctgcagt ccatttgatg tagacaagcc atctagtatg caaaagcaaa ctcaggttaa
1321 actaagaatc caaacagatg gcgataagta tggtggaacc tcaattcaac tccagttgaa
1381 agagaagaag aaaaaaggat attgtgaatg ttgcttgcag aaatatgaag atctagaaac
1441 tcaccttcta agtgagcaac acagaaactt tgcacagagt aaccagtatc aagttgttga
1501 tgatattgta tctaagttag tttttgactt tgtggaatat gaaaaggaca cacctaaaaa
1561 gaaaagaata aaatacagtg ttggatccct ttctcctgtt tctgcaagtg tcctgaaaaa
1621 gactgaacaa aaggaaaaag tggaattgca acatatttct cagaaagatt gccaggaaga
1681 tgatacaaca gtgaaggagc agaatttcct gtataaagag acccaggaaa ctgaaaaaaa
1741 gctcctgttt atttcagagc ccatccccca cccttcaaat gaattgagag ggcttaatga
1801 gaaaatgagt aataaatgtt ccatgttaag tacagctgaa gatgacataa gacagaattt
1861 tacacagcta cctctacata aaaacaaaca ggaatgcatt cttgacattt ccgaacacac
1921 attaagtgaa aatgacttag aagaactaag ggtagatcac tataaatgta acatacaggc
1981 atctgtacat gtttctgatt tcagtacaga taatagtgga tctcaaccaa aacagaagtc
2041 agatactgtg ctttttccag caaaggatct caaggaaaag gaccttcatt caatatttac
2101 tcatgattct ggtctgataa caataaacag ttcacaagag cacctaactg ttcaggcaaa
2161 ggctccattc catactcctc ctgaggaacc caatgaatgt gacttcaaga atatggatag
2221 tttaccttct ggtaaaatac atcgaaaagt gaaaataata ttaggacgaa atagaaaaga
2281 aaatctggaa ccaaatgctg aatttgataa aagaactgaa tttattacac aagaagaaaa
2341 cagaatttgt agttcaccgg tacagtcttt actagacttg tttcagacta gtgaagagaa
2401 atcagaattt ttgggtttca caagctacac agaaaagagt ggtatatgca atgttttaga
2461 tatttgggaa gaggaaaatt cagataatct gttaacagcg tttttctcgt ccccttcaac
2521 ttctacattt actggctttt agaatttaaa aaatgcatac ttttcagaag tgataaggat
2581 catattcttg aaatttttat aaatatgtat ggaaattctt aggatttttt taccagcttt
2641 gtttacagac ccaaatgtaa atattaaaaa taaatatttg caattttcta cagaattgaa
2701 tacctgttaa agaaaaatta cagaataaac ttgtgactgg tcttgtttta cattaaaaaa
2761 aaaaaaaaaa aaaactcgag
//