LOCUS AA993745 446 bp mRNA linear EST 08-JAN-2011 DEFINITION ot48d10.s1 Soares_testis_NHT Homo sapiens cDNA clone IMAGE:1620019 3' similar to SW:ADAA_MOUSE P17426 ALPHA-ADAPTIN, mRNA sequence. ACCESSION AA993745 VERSION AA993745.1 DBLINK BioSample: SAMN00155229 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 446) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov cDNA Library Preparation: M. Bento Soares, Ph.D., M. Fatima Bonaldo, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Insert Length: 492 Std Error: 0.00 Seq primer: -40m13 fwd. ET from Amersham High quality sequence stop: 261. FEATURES Location/Qualifiers source 1..446 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:1620019" /sex="male" /clone_lib="SAMN00155229 Soares_testis_NHT" /lab_host="DH10B" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was prepared from mRNA obtained from Clontech Laboratories, Inc., and primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCCAATTTTTTTTTTTTTTTTT 3']. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization to Cot5, and was constructed by Bento Soares and M. Fatima Bonaldo." BASE COUNT 97 a 115 c 146 g 88 t ORIGIN 1 tttgttagcg tctctctgtt tattcgctca caataataca cagcccctgg atggggaggg 61 ggtaggaggg gctacaacag ggtggggtgg gagggggaga ggttgatgaa cttgatgtag 121 gtggacagca gcagcgcccg cgtggccacg ctgcacagat ggaacttgga gtggagcagg 181 gagaactgca ctggatggct ggagcggggg tccccagcaa tcaagttccc aaactcccca 241 aggatgtagc cgccaacctt caccatgttc tcgtgacagg caggggcctg gagcgcctca 301 aagacggtct tggcggcata gccctggacg tcatcacggt tggtgacgat ctgtagcaca 361 cggtaccaca cctcctcact cacgtattcg cccgcaatgc gagtgaggtt gaggatggtg 421 tccacgtacc agctatagtc cacggc //