LOCUS       AA993745                 446 bp    mRNA    linear   EST 08-JAN-2011
DEFINITION  ot48d10.s1 Soares_testis_NHT Homo sapiens cDNA clone IMAGE:1620019
            3' similar to SW:ADAA_MOUSE P17426 ALPHA-ADAPTIN, mRNA sequence.
ACCESSION   AA993745
VERSION     AA993745.1
DBLINK      BioSample: SAMN00155229
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 446)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            cDNA Library Preparation: M. Bento Soares, Ph.D., M. Fatima
            Bonaldo, Ph.D.
             cDNA Library Arrayed by: Greg Lennon, Ph.D.
             DNA Sequencing by: Washington University Genome Sequencing Center
             Clone distribution: NCI-CGAP clone distribution information can be
            found through the I.M.A.G.E. Consortium/LLNL at:
            www-bio.llnl.gov/bbrp/image/image.html
            
            Insert Length: 492   Std Error: 0.00
            Seq primer: -40m13 fwd. ET from Amersham
            High quality sequence stop: 261.
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:1620019"
                     /sex="male"
                     /clone_lib="SAMN00155229 Soares_testis_NHT"
                     /lab_host="DH10B"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     1st strand cDNA was prepared from mRNA obtained from
                     Clontech Laboratories, Inc., and primed with a Not I -
                     oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCCCAATTTTTTTTTTTTTTTTT 3'].
                     Double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Not I and cloned into the Not I
                     and Eco RI sites of the modified pT7T3 vector. Library
                     went through one round of normalization to Cot5, and was
                     constructed by Bento Soares and M. Fatima Bonaldo."
BASE COUNT           97 a          115 c          146 g           88 t
ORIGIN      
        1 tttgttagcg tctctctgtt tattcgctca caataataca cagcccctgg atggggaggg
       61 ggtaggaggg gctacaacag ggtggggtgg gagggggaga ggttgatgaa cttgatgtag
      121 gtggacagca gcagcgcccg cgtggccacg ctgcacagat ggaacttgga gtggagcagg
      181 gagaactgca ctggatggct ggagcggggg tccccagcaa tcaagttccc aaactcccca
      241 aggatgtagc cgccaacctt caccatgttc tcgtgacagg caggggcctg gagcgcctca
      301 aagacggtct tggcggcata gccctggacg tcatcacggt tggtgacgat ctgtagcaca
      361 cggtaccaca cctcctcact cacgtattcg cccgcaatgc gagtgaggtt gaggatggtg
      421 tccacgtacc agctatagtc cacggc
//