LOCUS       AA827939                 500 bp    mRNA    linear   EST 07-JAN-2011
DEFINITION  od02c09.s1 NCI_CGAP_GCB1 Homo sapiens cDNA clone IMAGE:1358032 3'
            similar to TR:P70704 P70704 CHROMAFFIN GRANULE ATPASE II HOMOLOG,
            mRNA sequence.
ACCESSION   AA827939
VERSION     AA827939.1
DBLINK      BioSample: SAMN00155198
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 500)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: Louis M. Staudt, M.D., Ph.D., David Allman,
            Ph.D., Gerald Marti, M.D.
             cDNA Library Preparation: M. Bento Soares, Ph.D., M. Fatima
            Bonaldo, Ph.D.
             cDNA Library Arrayed by: Greg Lennon, Ph.D.
             DNA Sequencing by: Washington University Genome Sequencing Center
             Clone distribution: NCI-CGAP clone distribution information can be
            found through the I.M.A.G.E. Consortium/LLNL at:
            www-bio.llnl.gov/bbrp/image/image.html
            
            Insert Length: 336   Std Error: 0.00
            Seq primer: -40m13 fwd. ET from Amersham
            High quality sequence stop: 372.
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:1358032"
                     /tissue_type="germinal center B cell"
                     /clone_lib="SAMN00155198 NCI_CGAP_GCB1"
                     /lab_host="DH10B"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     1st strand cDNA was prepared from human tonsillar cells
                     enriched for germinal center B cells by flow sorting
                     (CD20+, IgD-), provided by Dr. Louis M. Staudt (NCI), Dr.
                     David Allman (NCI) and Dr. Gerald Marti (CBER). cDNA
                     synthesis was primed with a Not I - oligo(dT) primer
                     [5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCCTCATTTTTTTTTTTTTTTTTT-
                     3']. Double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Not I and cloned into the Not I
                     and Eco RI sites of the modified pT7T3 vector. Library
                     went through one round of normalization, and was
                     constructed by Bento Soares and M. Fatima Bonaldo."
BASE COUNT          122 a          127 c          140 g          111 t
ORIGIN      
        1 aaggtcaaga tgttctgcgt gacgtgcccg tcttgtccga gaagatgtat tccacctggc
       61 ccaggtggtc gttgaggctg gtgctgcggg ccttgcaggc acgtcctgcg gcttgtagta
      121 catctgcacg tcccagtcga tgaagacgct gttccccagg tagatgaact cggacaggat
      181 gaacatggac atcgggatgg tgacgctgag caggatgagg aagctccaga agacgaagaa
      241 ggactctgcg gccacgctgc tcccatgcac ccccgagagg tagtagtggt ggtctttgaa
      301 ttctttgact gagaaaccga agccgaacgc aacaccaggc agacaagcac cacggagatg
      361 aagatcacaa ccaccagctt gttcatcagg aggtccagct tggttctctt caaatggatc
      421 ttgccacagt tcctcataat ttttgtgtca aaaccagcat aaatgaccag tccattagca
      481 gtgtctgtgt tgcgaatccg
//