LOCUS AA827939 500 bp mRNA linear EST 07-JAN-2011 DEFINITION od02c09.s1 NCI_CGAP_GCB1 Homo sapiens cDNA clone IMAGE:1358032 3' similar to TR:P70704 P70704 CHROMAFFIN GRANULE ATPASE II HOMOLOG, mRNA sequence. ACCESSION AA827939 VERSION AA827939.1 DBLINK BioSample: SAMN00155198 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 500) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Louis M. Staudt, M.D., Ph.D., David Allman, Ph.D., Gerald Marti, M.D. cDNA Library Preparation: M. Bento Soares, Ph.D., M. Fatima Bonaldo, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Insert Length: 336 Std Error: 0.00 Seq primer: -40m13 fwd. ET from Amersham High quality sequence stop: 372. FEATURES Location/Qualifiers source 1..500 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:1358032" /tissue_type="germinal center B cell" /clone_lib="SAMN00155198 NCI_CGAP_GCB1" /lab_host="DH10B" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was prepared from human tonsillar cells enriched for germinal center B cells by flow sorting (CD20+, IgD-), provided by Dr. Louis M. Staudt (NCI), Dr. David Allman (NCI) and Dr. Gerald Marti (CBER). cDNA synthesis was primed with a Not I - oligo(dT) primer [5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCCTCATTTTTTTTTTTTTTTTTT- 3']. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo." BASE COUNT 122 a 127 c 140 g 111 t ORIGIN 1 aaggtcaaga tgttctgcgt gacgtgcccg tcttgtccga gaagatgtat tccacctggc 61 ccaggtggtc gttgaggctg gtgctgcggg ccttgcaggc acgtcctgcg gcttgtagta 121 catctgcacg tcccagtcga tgaagacgct gttccccagg tagatgaact cggacaggat 181 gaacatggac atcgggatgg tgacgctgag caggatgagg aagctccaga agacgaagaa 241 ggactctgcg gccacgctgc tcccatgcac ccccgagagg tagtagtggt ggtctttgaa 301 ttctttgact gagaaaccga agccgaacgc aacaccaggc agacaagcac cacggagatg 361 aagatcacaa ccaccagctt gttcatcagg aggtccagct tggttctctt caaatggatc 421 ttgccacagt tcctcataat ttttgtgtca aaaccagcat aaatgaccag tccattagca 481 gtgtctgtgt tgcgaatccg //