LOCUS AA813437 519 bp mRNA linear EST 08-JAN-2011 DEFINITION ai77h11.s1 Soares_testis_NHT Homo sapiens cDNA clone 1376901 3' similar to SW:VP40_BDV Q01552 40 KD PROTEIN. [2] SW:VP40_BDV, mRNA sequence. ACCESSION AA813437 VERSION AA813437.1 DBLINK BioSample: SAMN00155229 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 519) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov cDNA Library Preparation: M. Bento Soares, Ph.D., M. Fatima Bonaldo, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Possible reversed clone: similarity on wrong strand Insert Length: 1647 Std Error: 0.00 Seq primer: -40m13 fwd. ET from Amersham High quality sequence stop: 51. FEATURES Location/Qualifiers source 1..519 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="1376901" /sex="male" /clone_lib="SAMN00155229 Soares_testis_NHT" /lab_host="DH10B" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was prepared from mRNA obtained from Clontech Laboratories, Inc., and primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCCAATTTTTTTTTTTTTTTTT 3']. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization to Cot5, and was constructed by Bento Soares and M. Fatima Bonaldo." BASE COUNT 147 a 101 c 108 g 163 t ORIGIN 1 tttctatttt agaatgtgat tttcacataa tttcttttta tatgtatata taaggattag 61 ttcacgtatt tgttattcaa atcccgaaat cccataacgg cgaaggatgt taagtgtata 121 tggatccatg tcacttcctc tggagatttt ctgagcagat gcaatttgaa gtacagggaa 181 tgtaatagtt gatcctggta taatgtcaag ggcttcaaaa tccagaaaat gtggaattcc 241 ttcttttggc ccagtagttt gctgctgtgg ctagggtttg gggaacattt gtggtgatag 301 caccccaata acagggtggc gaagtacacc cccaaattca aagaaatctc caagtacctt 361 tttagccagt ggtttcttct gctcaaaaac tggaatctcc aacacaacag cgggtaaagc 421 agtggaacca tccacacact gattcaggaa cattctcaca gtgtagtagg tcgtcatctg 481 tgctttgctg gcaactactt taacttgatc aagtagctc //