LOCUS       AA813437                 519 bp    mRNA    linear   EST 08-JAN-2011
DEFINITION  ai77h11.s1 Soares_testis_NHT Homo sapiens cDNA clone 1376901 3'
            similar to SW:VP40_BDV Q01552 40 KD PROTEIN. [2] SW:VP40_BDV, mRNA
            sequence.
ACCESSION   AA813437
VERSION     AA813437.1
DBLINK      BioSample: SAMN00155229
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 519)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            cDNA Library Preparation: M. Bento Soares, Ph.D., M. Fatima
            Bonaldo, Ph.D.
             cDNA Library Arrayed by: Greg Lennon, Ph.D.
             DNA Sequencing by: Washington University Genome Sequencing Center
             Clone distribution: NCI-CGAP clone distribution information can be
            found through the I.M.A.G.E. Consortium/LLNL at:
            www-bio.llnl.gov/bbrp/image/image.html
            
            Possible reversed clone: similarity on wrong strand
            Insert Length: 1647   Std Error: 0.00
            Seq primer: -40m13 fwd. ET from Amersham
            High quality sequence stop: 51.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="1376901"
                     /sex="male"
                     /clone_lib="SAMN00155229 Soares_testis_NHT"
                     /lab_host="DH10B"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     1st strand cDNA was prepared from mRNA obtained from
                     Clontech Laboratories, Inc., and primed with a Not I -
                     oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCCCAATTTTTTTTTTTTTTTTT 3'].
                     Double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Not I and cloned into the Not I
                     and Eco RI sites of the modified pT7T3 vector. Library
                     went through one round of normalization to Cot5, and was
                     constructed by Bento Soares and M. Fatima Bonaldo."
BASE COUNT          147 a          101 c          108 g          163 t
ORIGIN      
        1 tttctatttt agaatgtgat tttcacataa tttcttttta tatgtatata taaggattag
       61 ttcacgtatt tgttattcaa atcccgaaat cccataacgg cgaaggatgt taagtgtata
      121 tggatccatg tcacttcctc tggagatttt ctgagcagat gcaatttgaa gtacagggaa
      181 tgtaatagtt gatcctggta taatgtcaag ggcttcaaaa tccagaaaat gtggaattcc
      241 ttcttttggc ccagtagttt gctgctgtgg ctagggtttg gggaacattt gtggtgatag
      301 caccccaata acagggtggc gaagtacacc cccaaattca aagaaatctc caagtacctt
      361 tttagccagt ggtttcttct gctcaaaaac tggaatctcc aacacaacag cgggtaaagc
      421 agtggaacca tccacacact gattcaggaa cattctcaca gtgtagtagg tcgtcatctg
      481 tgctttgctg gcaactactt taacttgatc aagtagctc
//