LOCUS       AA621632                 516 bp    mRNA    linear   EST 14-OCT-1997
DEFINITION  af48c06.s1 Soares_total_fetus_Nb2HF8_9w Homo sapiens cDNA clone
            IMAGE:1034890 3' similar to gb:M61900 Human prostaglandin D
            synthase gene, complete cds. (HUMAN), mRNA sequence.
ACCESSION   AA621632
VERSION     AA621632.1
DBLINK      BioSample: SAMN00155442
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Hillier,L., Allen,M., Bowles,L., Dubuque,T., Geisel,G., Jost,S.,
            Krizman,D., Kucaba,T., Lacy,M., Le,N., Lennon,G., Marra,M.,
            Martin,J., Moore,B., Schellenberg,K., Steptoe,M., Tan,F.,
            Theising,B., White,Y., Wylie,T., Waterston,R. and Wilson,R.
  TITLE     WashU-NCI human EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Seq primer: -40m13 fwd. ET from Amersham
            High quality sequence stop: 386.
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:1034890"
                     /clone_lib="SAMN00155442 Soares_total_fetus_Nb2HF8_9w"
                     /dev_stage="8-9 weeks"
                     /lab_host="DH10B"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     1st strand cDNA was prepared from mRNA obtained from
                     pooled 8-9 week (total) fetus material with a Not I -
                     oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCTTAATTTTTTTTTTTTTTTTT 3'].
                     Double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Not I and cloned into the Not I
                     and Eco RI sites of the modified pT7T3 vector. Library
                     went through one round of normalization, and was
                     constructed by Bento Soares and M. Fatima Bonaldo."
BASE COUNT           88 a          123 c          181 g          124 t
ORIGIN      
        1 tttttttttt ttttttactg acttgcttcc ggagtttatt gtgcaggatg aagcctgagt
       61 gggcagggtt gctttggcgg ggagccgggg caggggctcg gggaaggaac agagcagaga
      121 catccagagc gtgggggtca cctggctggc cgggatccca gcttcagccc tggggagtcc
      181 tattgttccg tcatgcactt atcggtttgg ggcaggaaga caatggtatc ctctgtgaag
      241 ccctgggcct tgcagaaggc ggtgaatttc tcctttaact cagccctggg ggtctgggtt
      301 cggctgtaga gggtggccat gcggaagtcc tcgccagggc ccttgctgcc ctggctgtac
      361 agcagcgcgt actggtcgta gtcggtctcc accactgaca cggagtaggt gctgccccag
      421 tgggagctcc ggtagctgta ggagccgagg gacccgcggc tgaagagcat ggttccggtc
      481 tcacacgggt tatctgagaa ggtgaagtca ggttga
//