LOCUS AA496212 576 bp mRNA linear EST 11-AUG-1997 DEFINITION zx70c10.r1 Soares_total_fetus_Nb2HF8_9w Homo sapiens cDNA clone IMAGE:796818 5', mRNA sequence. ACCESSION AA496212 VERSION AA496212.1 DBLINK BioSample: SAMN00155442 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 576) AUTHORS Hillier,L., Allen,M., Bowles,L., Dubuque,T., Geisel,G., Jost,S., Kucaba,T., Lacy,M., Le,N., Lennon,G., Marra,M., Martin,J., Moore,B., Schellenberg,K., Steptoe,M., Tan,F., Theising,B., White,Y., Wylie,T., Waterston,R. and Wilson,R. TITLE WashU-Merck EST Project 1997 JOURNAL Unpublished COMMENT Contact: Wilson RK Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: est@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Seq primer: -28m13 rev2 ET from Amersham High quality sequence stop: 513. FEATURES Location/Qualifiers source 1..576 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="GDB:6041377" /db_xref="taxon:9606" /clone="IMAGE:796818" /clone_lib="SAMN00155442 Soares_total_fetus_Nb2HF8_9w" /dev_stage="8-9 weeks" /lab_host="DH10B" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was prepared from mRNA obtained from pooled 8-9 week (total) fetus material with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCTTAATTTTTTTTTTTTTTTTT 3']. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo." BASE COUNT 131 a 137 c 166 g 142 t ORIGIN 1 gtcccactgt cctggctccg tgcgtccagt gagattgggc ctgggcgctg gagctgctgt 61 ggctcccgcc gcggcgctgc tcatggaggc catgcagagc ccagaactca cgccggggga 121 ggccgagaca gccggcggta ctcatagatg aggcagcggc ggcgcggggc ggcggcagcc 181 gggctctcca tgagcaggcg gcggcggcga cgggctgcgg cggcacggca gttttcggtc 241 cccagggagg atgaagacac tgtttgaaga gatcaaagca tcaattagaa ataactataa 301 ccaagatcga tcattttgta ggcctgttct tccttggggg ggtgttttta ctatcaaagc 361 tggccgcaaa gcagtatcct gtacaccact ctatgttgaa ataagactga gaaatacctg 421 caccatagat ggattcttga tgttattata tgtcattctt aatgaaaatg aaaacttccc 481 tagggaactc tctcttcatt ttggtagaga gtttgtagac tgttttcttt acttaatgga 541 cacctacagt tttacaactg tgaagctact ttggat //