LOCUS       AA496212                 576 bp    mRNA    linear   EST 11-AUG-1997
DEFINITION  zx70c10.r1 Soares_total_fetus_Nb2HF8_9w Homo sapiens cDNA clone
            IMAGE:796818 5', mRNA sequence.
ACCESSION   AA496212
VERSION     AA496212.1
DBLINK      BioSample: SAMN00155442
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Hillier,L., Allen,M., Bowles,L., Dubuque,T., Geisel,G., Jost,S.,
            Kucaba,T., Lacy,M., Le,N., Lennon,G., Marra,M., Martin,J.,
            Moore,B., Schellenberg,K., Steptoe,M., Tan,F., Theising,B.,
            White,Y., Wylie,T., Waterston,R. and Wilson,R.
  TITLE     WashU-Merck EST Project 1997
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Seq primer: -28m13 rev2 ET from Amersham
            High quality sequence stop: 513.
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:6041377"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:796818"
                     /clone_lib="SAMN00155442 Soares_total_fetus_Nb2HF8_9w"
                     /dev_stage="8-9 weeks"
                     /lab_host="DH10B"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     1st strand cDNA was prepared from mRNA obtained from
                     pooled 8-9 week (total) fetus material with a Not I -
                     oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCTTAATTTTTTTTTTTTTTTTT 3'].
                     Double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Not I and cloned into the Not I
                     and Eco RI sites of the modified pT7T3 vector. Library
                     went through one round of normalization, and was
                     constructed by Bento Soares and M. Fatima Bonaldo."
BASE COUNT          131 a          137 c          166 g          142 t
ORIGIN      
        1 gtcccactgt cctggctccg tgcgtccagt gagattgggc ctgggcgctg gagctgctgt
       61 ggctcccgcc gcggcgctgc tcatggaggc catgcagagc ccagaactca cgccggggga
      121 ggccgagaca gccggcggta ctcatagatg aggcagcggc ggcgcggggc ggcggcagcc
      181 gggctctcca tgagcaggcg gcggcggcga cgggctgcgg cggcacggca gttttcggtc
      241 cccagggagg atgaagacac tgtttgaaga gatcaaagca tcaattagaa ataactataa
      301 ccaagatcga tcattttgta ggcctgttct tccttggggg ggtgttttta ctatcaaagc
      361 tggccgcaaa gcagtatcct gtacaccact ctatgttgaa ataagactga gaaatacctg
      421 caccatagat ggattcttga tgttattata tgtcattctt aatgaaaatg aaaacttccc
      481 tagggaactc tctcttcatt ttggtagaga gtttgtagac tgttttcttt acttaatgga
      541 cacctacagt tttacaactg tgaagctact ttggat
//