LOCUS AA486291 419 bp mRNA linear EST 06-MAR-1998
DEFINITION ab35a01.r1 Stratagene HeLa cell s3 937216 Homo sapiens cDNA clone
IMAGE:842760 5' similar to TR:G529900 G529900 TWISTED GASTRULATION,
mRNA sequence.
ACCESSION AA486291
VERSION AA486291.1
DBLINK BioSample: SAMN00155133
KEYWORDS EST.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 419)
AUTHORS Hillier,L., Allen,M., Bowles,L., Dubuque,T., Geisel,G., Jost,S.,
Krizman,D., Kucaba,T., Lacy,M., Le,N., Lennon,G., Marra,M.,
Martin,J., Moore,B., Schellenberg,K., Steptoe,M., Tan,F.,
Theising,B., White,Y., Wylie,T., Waterston,R. and Wilson,R.
TITLE WashU-NCI human EST Project
JOURNAL Unpublished
COMMENT Contact: Wilson RK
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
Tel: 314 286 1800
Fax: 314 286 1810
Email: est@watson.wustl.edu
This clone is available royalty-free through LLNL ; contact the
IMAGE Consortium (info@image.llnl.gov) for further information.
Insert Length: 1747 Std Error: 0.00
Seq primer: -28m13 rev1 ET from Amersham
High quality sequence stop: 401.
FEATURES Location/Qualifiers
source 1..419
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:842760"
/sex="female"
/clone_lib="SAMN00155133 Stratagene HeLa cell s3 937216"
/dev_stage="HeLa S3 cell line"
/lab_host="SOLR (kanamycin resistant)"
/note="Vector: pBluescript SK-; Site_1: EcoRI; Site_2:
XhoI; Cloned unidirectionally. Primer: Oligo dT. HeLa S3
epithelioid carcinoma cells grown to semi-confluency
without induction. Average insert size: 1.5 kb; Uni-ZAP XR
Vector.
5' adaptor sequence: 5' GAATTCGGCACGAG 3'
3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'"
BASE COUNT 127 a 85 c 84 g 122 t
ORIGIN
1 tcgtgtcntc ttccgggcac tcacagaagg agatactcag ttgaattgga acatcgtttc
61 tttccctgtt gcagaagaac tttcacatca tgagaatctg gtttcatttt tagaaactgt
121 gaaccagcca caccaccaga atgtgtctgt ccccagcaat aatgttcacg cgccttattc
181 cagtgacaaa gaacacatgt gtactgtggt ttattttgat gactgcatgt ccatacatca
241 gtgtaaaata tcctgtgagt ccatgggagc atccaaatat cgctggtttc ataatgcctg
301 ctgcgagtgc attggtccag aatgtattga ctatggtagt aaaactgtca aatgtatgaa
361 ctgcatgttt taaagaagac aaatgcaaac caaagcaact tagtaaaata ataggtata
//