LOCUS Z32699 5748 bp DNA linear SYN 14-NOV-2006
DEFINITION Expression vector (PAN52-4) DNA 5748bp.
ACCESSION Z32699
VERSION Z32699.1
KEYWORDS gdpA gene.
SOURCE synthetic construct
ORGANISM synthetic construct
other sequences; artificial sequences.
REFERENCE 1 (bases 999 to 2572)
AUTHORS Punt P.J., Dingemanse M.A., Kuyvenhoven A., Soede R.D.M.,
Pouwels P.H., van den Hondel C.A.M.J.J.
TITLE Functional elements in the promoter region of the Aspergillus
nidulans gpdA gene encoding glyceraldehyde-3-phosphate
dehydrogenase
JOURNAL Gene 93(1), 101-109(1990).
PUBMED 2121607
REFERENCE 2
AUTHORS Punt P.J., Kuijvenhoven A., Broekhuijsen M.P.,
van den Hondel C.A.M.J.J.
TITLE Vectors for efficient production of homologous and heterologous
proteins in filamentous fungi
JOURNAL Unpublished.
REMARK (sites)
REFERENCE 3 (bases 2305 to 3071)
AUTHORS Mullaney E.J., Hamer J.E., Roberti K.A., Yelton M.M.,
Timberlake W.E.
TITLE Primary structure of the trpC gene from Aspergillus nidulans
JOURNAL Mol. Gen. Genet. 199(1), 37-45(1985).
PUBMED 3158796
REFERENCE 4 (bases 1 to 5748)
AUTHORS Punt P.
JOURNAL Submitted (22-APR-1994) to the INSDC. Punt P., TNO Nutrition and
Food Research Institute, Lange Kleiweg 139, Rijkswik, The
Netherlands.
FEATURES Location/Qualifiers
source 1..5748
/organism="synthetic construct"
/mol_type="other DNA"
/db_xref="taxon:32630"
regulatory 1..2129
/function="promoter region of the A.nidulans gpdA gene"
/citation=[1]
/citation=[2]
/experiment="experimental evidence, no additional details
recorded"
/regulatory_class="promoter"
misc_feature 1..5748
/product="fungal expression/SECRETION vector pAN52-4"
/citation=[2]
/experiment="experimental evidence, no additional details
recorded"
misc_feature 2130
/gene="gpdA"
/function="major transcription start site"
/citation=[1]
/experiment="experimental evidence, no additional details
recorded"
intron 2178..2293
/number=1
/citation=[1]
/experiment="experimental evidence, no additional details
recorded"
CDS 2302..2391
/transl_table=11
/product="synthetic signal sequence"
/function="encodes secretion signal sequence"
/note="The sequence is very similar to that of the A.niger
glucoamylase gene"
/citation=[2]
/experiment="experimental evidence, no additional details
recorded"
/protein_id="CAA83648.1"
/translation="MGFRSLLALSGLVCNGLANVISKRAGSEA"
sig_peptide 2302..2355
/product="synthetic signal sequence"
/note="sequence very similar to that of the signal
sequence of the A.niger glucoamylase gene (accession #
X00712)"
/citation=[2]
misc_feature 2356..2375
/product="PRO_PEPTIDE"
/note="sequence encoding putative propeptide, contains
dibasic processing site (LYS-ARG)"
/citation=[2]
regulatory 2392..3115
/standard_name="terminator region from A.nidulans trpC
gene (containing CDS)"
/note="this region contains the 3'end of the trpC
transcript including 50 codons. two major transcription
ends have been identified"
/citation=[2]
/citation=[3]
/experiment="experimental evidence, no additional details
recorded"
/regulatory_class="terminator"
polyA_site 2642..2652
misc_difference 2652..2662
/replace="gccttncaggc"
/note="introduction of one base at this position generates
an BLGI site with was shown to be present by restriction
digestion"
/citation=[3]
misc_difference 2861..2869
/replace="cagcncctg"
/note="introduction of one base at this position generates
an ALWNI site which was shown to be present by restriction
digestion"
/citation=[3]
polyA_site 2915..2925
misc_difference 2949..2959
/replace="gaaatcanttc"
/note="introduction (actual sequence could be deletion as
well) of a base at this position results in the
elimination of an xmni site which was shown to be absent
by restriction digestion"
/citation=[3]
variation 2986..2993
/replace="catgcatg"
/note="A NCOI site present at this position was removed by
digestion, treatment with klenow polymerase and
religation"
/citation=[2]
misc_feature 3115..5748
/product="PUC18"
/note="vector sequences derived from PUC18 from HINDIII to
ECORI"
/citation=[2]
BASE COUNT 1435 a 1454 c 1387 g 1469 t
ORIGIN
1 gaattccctt gtatctctac acacaggctc aaatcaataa gaagaacggt tcgtcttttt
61 cgtttatatc ttgcatcgtc ccaaagctat tggcgggata ttctgtttgc agttggctga
121 cttgaagtaa tctctgcaga tctttcgaca ctgaaatacg tcgagcctgc tccgcttgga
181 agcggcgagg agcctcgtcc tgtcacaact accaacatgg agtacgataa gggccagttc
241 cgccagctca ttaagagcca gttcatgggc gttggcatga tggccgtcat gcatctgtac
301 ttcaagtaca ccaacgctct tctgatccag tcgatcatcc gctgaaggcg ctttcgaatc
361 tggttaagat ccacgtcttc gggaagccag cgactggtga cctccagcgt ccctttaagg
421 ctgccaacag ctttctcagc cagggccagc ccaagaccga caaggcctcc ctccagaacg
481 ccgagaagaa ctggaggggt ggtgtcaagg aggagtaagc tccttattga agtcggagga
541 cggagcggtg tcaagaggat attcttcgac tctgtattat agataagatg atgaggaatt
601 ggaggtagca tagcttcatt tggatttgct ttccaggctg agactctagc ttggagcata
661 gagggtcctt tggctttcaa tattctcaag tatctcgagt ttgaacttat tccctgtgaa
721 ccttttattc accaatgagc attggaatga acatgaatct gaggactgca atcgccatga
781 ggttttcgaa atacatccgg atgtcgaagg cttggggcac ctgcgttggt tgaatttaga
841 acgtggcact attgatcatc cgatagctct gcaaagggcg ttgcacaatg caagtcaaac
901 gttgctagca gttccaggtg gaatgttatg atgagcattg tattaaatca ggagatatag
961 catgatctct agttagctca ccacaaaagt cagacggcgt aaccaaaagt cacacaacac
1021 aagctgtaag gatttcggca cggctacgga agacggagaa gccaccttca gtggactcga
1081 gtaccattta attctatttg tgtttgatcg agacctaata cagcccctac aacgaccatc
1141 aaagtcgtat agctaccagt gaggaagtgg actcaaatcg acttcagcaa catctcctgg
1201 ataaacttta agcctaaact atacagaata agataggtgg agagcttata ccgagctccc
1261 aaatctgtcc agatcatggt tgaccggtgc ctggatcttc ctatagaatc atccttattc
1321 gttgacctag ctgattctgg agtgacccag agggtcatga cttgagccta aaatccgccg
1381 cctccaccat ttgtagaaaa atgtgacgaa ctcgtgagct ctgtacagtg accggtgact
1441 ctttctggca tgcggagaga cggacggacg cagagagaag ggctgagtaa taagccactg
1501 gccagacagc tctggcggct ctgaggtgca gtggatgatt attaatccgg gaccggccgc
1561 ccctccgccc cgaagtggaa aggctggtgt gcccctcgtt gaccaagaat ctattgcatc
1621 atcggagaat atggagcttc atcgaatcac cggcagtaag cgaaggagaa tgtgaagcca
1681 ggggtgtata gccgtcggcg aaatagcatg ccattaacct aggtacagaa gtccaattgc
1741 ttccgatctg gtaaaagatt cacgagatag taccttctcc gaagtaggta gagcgagtac
1801 ccggcgcgta agctccctaa ttggcccatc cggcatctgt agggcgtcca aatatcgtgc
1861 ctctcctgct ttgcccggtg tatgaaaccg gaaaggccgc tcaggagctg gccagcggcg
1921 cagaccggga acacaagctg gcagtcgacc catccggtgc tctgcactcg acctgctgag
1981 gtccctcagt ccctggtagg cagctttgcc ccgtctgtcc gcccggtgtg tcggcggggt
2041 tgacaaggtc gttgcgtcag tccaacattt gttgccatat tttcctgctc tccccaccag
2101 ctgctctttt cttttctctt tcttttccca tcttcagtat attcatcttc ccatccaaga
2161 acctttattt cccctaagta agtactttgc tacatccata ctccatcctt cccatccctt
2221 attcctttga acctttcagt tcgagctttc ccacttcatc gcagcttgac taacagctac
2281 cccgcttgag cagacatcac catgggcttc cgatctctac tcgccctgag cggcctcgtc
2341 tgcaacgggt tggcaaatgt gatttccaag cgcgctggat ccgaagcttg agatccactt
2401 aacgttactg aaatcatcaa acagcttgac gaatctggat ataagatcgt tggtgtcgat
2461 gtcagctccg gagttgagac aaatggtgtt caggatctcg ataagatacg ttcatttgtc
2521 caagcagcaa agagtgcctt ctagtgattt aatagctcca tgtcaacaag aataaaacgc
2581 gttttcgggt ttacctcttc cagatacagc tcatctgcaa tgcattaatg cattgactgc
2641 aacctagtaa cgccttncag gctccggcga agagaagaat agcttagcag agctattttc
2701 attttcggga gacgagatca agcagatcaa cggtcgtcaa gagacctacg agactgagga
2761 atccgctctt ggctccacgc gactatatat ttgtctctaa ttgtactttg acatgctcct
2821 cttctttact ctgatagctt gactatgaaa attccgtcac cagcncctgg gttcgcaaag
2881 ataattgcat gtttcttcct tgaactctca agcctacagg acacacattc atcgtaggta
2941 taaacctcga aatcanttcc tactaagatg gtatacaata gtaaccatgc atggttgcct
3001 agtgaatgct ccgtaacacc caatacgccg gccgaaactt ttttacaact ctcctatgag
3061 tcgtttaccc agaatgcaca ggtacacttg tttagaggta atccttcttt ctagagcttg
3121 gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
3181 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
3241 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
3301 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
3361 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
3421 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
3481 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
3541 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
3601 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
3661 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
3721 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
3781 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
3841 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
3901 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
3961 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
4021 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
4081 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
4141 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
4201 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
4261 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
4321 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
4381 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
4441 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
4501 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
4561 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
4621 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
4681 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
4741 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
4801 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
4861 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
4921 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
4981 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
5041 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
5101 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
5161 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
5221 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
5281 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
5341 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
5401 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
5461 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
5521 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
5581 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
5641 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
5701 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattac
//