LOCUS X65332 2462 bp DNA circular SYN 25-JAN-2000
DEFINITION Cloning vector pSP72.
ACCESSION X65332
VERSION X65332.2
KEYWORDS beta-lactamase; bla gene; cloning vector; multiple cloning site;
promoter.
SOURCE Cloning vector pSP72
ORGANISM Cloning vector pSP72
other sequences; artificial sequences; vectors.
REFERENCE 1
AUTHORS Technical Services.
JOURNAL Submitted (23-MAR-1992) to the INSDC. Technical Services, Promega
Corporation, 2800 Woods Hollow Road, Madison, Wi 53711-5399, USA
REMARK revised by [2]
REFERENCE 2
AUTHORS Technical Services.
JOURNAL Submitted (28-MAY-1993) to the INSDC. Technical Services, Promega
Corporation, 2800 Woods Hollow Road, Madison, Wi 53711-5399, USA
REMARK revised by [3]
REFERENCE 3 (bases 1 to 2462)
AUTHORS Technical Services.
JOURNAL Submitted (12-JAN-2000) to the INSDC. Technical Services, Promega
Corporation, 2800 Woods Hollow Road, Madison, Wi 53711-5399, USA
COMMENT See X65300-X65335 for related vector sequences
This vector can be obtained from Promega Corporation, Madison, WI.
Call one of the following numbers for order or technical
information:
Order or Technical 800-356-9526
In Wisconsin 800-356-9526
Outside U.S. 608-274-4330
FEATURES Location/Qualifiers
source 1..2462
/organism="Cloning vector pSP72"
/mol_type="other DNA"
/db_xref="taxon:90137"
misc_feature 1
/note="SP6 transcription initiation site"
regulatory join(2446..2462,1..3)
/note="SP6 promoter"
/regulatory_class="promoter"
misc_feature 4..90
/note="multiple cloning sites"
regulatory 99..118
/note="T7 promoter"
/regulatory_class="promoter"
misc_feature 101
/note="T7 transcription initiation site"
CDS complement(1135..1995)
/transl_table=11
/gene="bla"
/product="Beta-lactamase"
/protein_id="CAA46432.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 624 a 614 c 605 g 619 t
ORIGIN
1 gaactcgagc agctgaagct tgcatgcctg caggtcgact ctagaggatc cccgggtacc
61 gagctcgaat tcatcgatga tatcagatct gccggtctcc ctatagtgag tcgtattaat
121 ttcgataagc caggttaacc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
181 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
241 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
301 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
361 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
421 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
481 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
541 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
601 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
661 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
721 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
781 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
841 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
901 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
961 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
1021 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
1081 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
1141 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
1201 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
1261 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
1321 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
1381 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
1441 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
1501 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
1561 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
1621 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
1681 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
1741 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
1801 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
1861 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
1921 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
1981 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
2041 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
2101 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
2161 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
2221 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
2281 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
2341 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atggacatat tgtcgttaga
2401 acgcggctac aattaataca taaccttatg tatcatacac atacgattta ggtgacacta
2461 ta
//