LOCUS X65277 2760 bp DNA linear BCT 14-NOV-2006 DEFINITION Pseudomonas aeruginosa nosZ, nosD and nosR genes, strain DSM 50071. ACCESSION X65277 VERSION X65277.1 KEYWORDS nitrous-oxide reductase; nosD gene; nosR gene; nosZ gene. SOURCE Pseudomonas aeruginosa ORGANISM Pseudomonas aeruginosa Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. REFERENCE 1 (bases 1 to 2760) AUTHORS Zumft W.G., Dreusch A., Lochelt S., Cuypers H., Friedrich B., Schneider B. TITLE Derived amino acid sequences of the nosZ gene (respiratory N2O reductase) from Alcaligenes eutrophus, Pseudomonas aeruginosa and Pseudomonas stutzeri reveal potential copper-binding residues. Implications for the CuA site of N2O reductase and cytochrome-c oxidase JOURNAL Eur. J. Biochem. 208(1), 31-40(1992). PUBMED 1324835 REFERENCE 2 (bases 1 to 2760) AUTHORS Zumft W.G., Viebrock-Sambale A., Braun C. TITLE Nitrous oxide reductase from denitrifying Pseudomonas stutzeri. Genes for copper-processing and properties of the deduced products, including a new member of the family of ATP/GTP-binding proteins JOURNAL Eur. J. Biochem. 192(3), 591-599(1990). PUBMED 2170125 REFERENCE 4 (bases 1 to 2760) AUTHORS Zumft W.G. JOURNAL Submitted (25-JUN-1992) to the INSDC. Zumft W. G., Universitaet Karlsruhe, Department of Microbiology, Kaiserstrasse 12, D-76128, Karlsruhe, GERMANY FEATURES Location/Qualifiers source 1..2760 /organism="Pseudomonas aeruginosa" /strain="DSM 50071" /mol_type="genomic DNA" /db_xref="taxon:287" CDS <1..303 /codon_start=1 /transl_table=11 /gene="nosR" /product="NosR protein" /function="regulatory protein for nitrous oxide reductase expression" /note="Derived protein fragment is homologous to the C-terminus of a regulatory component from Pseudomonas stutzeri (Cuypers et al.,1992)" /db_xref="GOA:Q01709" /db_xref="UniProtKB/Swiss-Prot:Q01709" /protein_id="CAA46380.1" /translation="CRYICPLGAALAIPSKFRLFDWLKRRKECGNPCQLCAKECEIQA IHPDGRINGNECHYCLDCQMTYHNDNKCPPLINKRKKRGKKAADPQLIPAVEVSDA" stem_loop 289..332 /function="putative transcriptional terminator" regulatory 348..354 /regulatory_class="ribosome_binding_site" CDS 359..2263 /transl_table=11 /gene="nosZ" /product="nitrous-oxide reductase" /function="respiratory oxidoreductase of denitrification" /EC_number="1.7.99.6" /db_xref="GOA:Q01710" /db_xref="InterPro:IPR001505" /db_xref="InterPro:IPR002429" /db_xref="InterPro:IPR006311" /db_xref="InterPro:IPR008972" /db_xref="InterPro:IPR011045" /db_xref="InterPro:IPR015943" /db_xref="InterPro:IPR023644" /db_xref="InterPro:IPR034205" /db_xref="InterPro:IPR041114" /db_xref="InterPro:IPR041142" /db_xref="UniProtKB/Swiss-Prot:Q01710" /protein_id="CAA46381.1" /translation="MSDKQTDKDEKTGLSRRGFLGASALTRSAVAASGLVGGVMTRDS WAAAAKEAQQKIHVAPGELDEYYGFWSGGHQGEVRVLGVPSMRELMRIPVFNVDSATG WGLTNESRHILGDTAKFLNGDCHHPHISMTDGKYDGKYLFINDKANSRVARIRLDIMK CDKITTIPNVQAIHGLRLQKVPHTKYVFCNAEFIIPHPNDGKVFDLEDENSFTMYNAV DAETMEVAFQVIVDGNLDNTDADYTGRFTAATCYNSEKAFDLGGMMRNERDWVVVFDI SAVEKEIKAGRFITLGDSKVPVVDGRKKDGKDSVVTRYIPVPKNPHGLNTSTDGKYFI ANGKLSPTCSMIAIDLLPDLFAGKLKDPRDVVVGEPELGLGPLHTTFDGRGNAYTTLF IDSQVVKWNMEEARRAYKGEKVNYIKQKLDVHYQPGHLHASLCETSEADGKWLVALSK FSKDRFLPTGPLHPENDQLIDISGDVMKLVHDGPTFAEPHDCIMARRDQIKTRKIWDR NDPFFAPTVAMAKKDGINLEEDNKVIRDGNKVRVYMTSMAPAYGLTEFKVKQGNEVTV VITNMDQIEDVSHGFVMVNHGVSMEISPQQTSSITFIADKPGLHWYYCSWFCHALHME MVGRMMVEPA" misc_feature 2084..2233 /function="putative copper-binding site" /note="label:CuA-site" /note="this domain shows structural similarity to the putative CuA-binding site of the subunit II of cytochrome c oxidase, EC 1.9.3.1" stem_loop 2267..2299 /function="putative transcriptional terminator" regulatory 2422..2425 /regulatory_class="ribosome_binding_site" CDS 2434..>2760 /transl_table=11 /gene="nosD" /product="NosD protein" /function="copper-processing component" /note="derived protein fragment is homologous to the N-terminus of a protein from Pseudomonas stutzeri involved in copper processing (Zumft et al.,1990)" /db_xref="GOA:Q01708" /db_xref="InterPro:IPR011050" /db_xref="UniProtKB/Swiss-Prot:Q01708" /protein_id="CAA46382.1" /translation="MIRYTKLPATCAVLLLAAGSVMAQVQPISTLPLQAQGENRWLLP AGEYHGQFVIDQPMQLRCAAGACCGPTGQGSALIIEASDVSVEGCTLLNWGRDLTAMD AGVHIAA" BASE COUNT 572 a 910 c 841 g 437 t ORIGIN 1 tgccgctaca tctgcccgct gggcgctgcc ctggcgatcc ccagcaagtt ccgcctgttc 61 gactggctca agcgccgcaa ggaatgcggc aacccctgcc agctgtgcgc caaagagtgc 121 gagatccagg ccatccaccc ggacggtcgc atcaacggca acgagtgcca ctactgcctc 181 gactgccaga tgacctacca caacgacaac aaatgcccgc cgctgatcaa caagcgcaag 241 aagcgcggca agaaggctgc cgacccacaa ctgattcccg ccgtcgaggt gagcgatgcc 301 tgaccacgag ccgaaccggg ctcgcggcag gcgtgttatt tcccaacagg agcgacccat 361 gagcgacaag caaactgata aggatgaaaa aaccggtctg agccggcgcg gctttcttgg 421 cgccagcgca cttaccaggt cggccgtggc ggccagtggc ctggtcggtg gggtgatgac 481 ccgcgacagc tgggcagcgg ctgccaagga ggcgcagcag aagatccatg tcgcccccgg 541 cgagctggac gagtactacg gcttctggag cggtggtcac cagggcgagg tgcgcgtgct 601 cggcgtgccg tcgatgcgcg agctgatgcg catcccggtg ttcaacgtcg attcggcgac 661 cggctggggc ctgaccaacg agagccggca catcctcggc gacacggcca agttcctcaa 721 cggcgactgc caccacccgc atatctccat gaccgacggc aagtacgacg gcaagtacct 781 gttcatcaac gacaaggcca acagccgcgt cgcgcgtatc cgcctggaca tcatgaagtg 841 cgacaagatc accaccatcc ccaacgtcca ggccatccat ggcctgcgct tgcagaaggt 901 gccgcacacc aagtacgtgt tctgcaacgc cgagttcatc atcccgcacc ccaatgacgg 961 caaggtcttc gacctcgagg acgagaacag cttcaccatg tacaacgccg tggatgccga 1021 gaccatggaa gtcgccttcc aggtcatcgt cgacggcaat ctcgacaaca ccgatgccga 1081 ctacaccggc cgtttcaccg ccgccacctg ctacaactcg gagaaggcct tcgacctcgg 1141 cggcatgatg cgcaacgagc gcgactgggt ggtggtgttc gacatctcgg cggtggagaa 1201 ggagatcaag gctggccgct tcatcaccct cggcgactcc aaggtgccgg tggtcgacgg 1261 gcgcaagaag gacggcaagg acagcgtggt gacccgctac attccggtgc cgaagaaccc 1321 gcacggcctg aacacttcca ccgacggcaa gtacttcatc gccaacggca agctgtcgcc 1381 gacctgctcg atgatcgcca tcgacctgct gcccgacctg ttcgccggca agctcaagga 1441 cccgcgtgac gtggtggtcg gcgagcccga gctgggcctt ggcccgctgc acaccacctt 1501 cgatggtcgc ggcaacgcct acaccacgct gttcatcgac agccaggtgg tcaagtggaa 1561 catggaggag gcccgtcgcg cctacaaggg cgagaaggtc aactacatca agcagaagct 1621 tgacgtgcac taccagccgg gtcacctgca cgcctcgctg tgcgaaacca gcgaagccga 1681 tggcaaatgg ctggtggcgc tgtcgaagtt ctccaaggac cgcttcctgc ccaccggccc 1741 gctgcacccg gagaatgacc agttgatcga catctccggc gacgtgatga agctggtgca 1801 tgacggcccg accttcgccg aaccgcatga ctgcatcatg gcccgccgcg accagatcaa 1861 gacgcgcaag atctgggacc gcaacgaccc tttcttcgcc ccgaccgtgg ccatggccaa 1921 gaaggacggc atcaacctgg aggaggacaa caaggtcatc cgcgatggca acaaggtgcg 1981 cgtgtacatg acctccatgg cgcccgccta tggtctgacc gaattcaagg tcaagcaggg 2041 caatgaagtc accgtggtga tcaccaacat ggaccagatc gaggacgtgt cccacggctt 2101 cgtcatggtc aaccacggcg tgagcatgga gatcagcccg cagcagacct cgtcgatcac 2161 cttcatcgcc gacaagcccg gcctgcactg gtactactgc agctggttct gccacgcgct 2221 gcacatggag atggtcggcc gcatgatggt cgagccggcc tgatgccggg gccggggagg 2281 attgccttcc ccggcctcgt attgagcttc agcgtgggag cgacgggggc gcctagcctt 2341 gtccgcgaat gcttgtggat aaacgttcgc ggagccccgt cgcttccaca aaacgtgatg 2401 cagcgtacga acaggtggac gaaggtaagt gcagtgatcc gatataccaa gctccccgcg 2461 acctgcgccg tgcttttgct cgctgcaggc tccgtcatgg cacaggtgca gcccatctcg 2521 accctgccgc tgcaggcgca gggcgagaac cgctggctgc tgccggcggg cgagtaccac 2581 gggcagttcg tcatcgacca gcccatgcaa ctgcgttgtg cggccggcgc gtgctgcggg 2641 ccgaccgggc agggcagcgc gttgatcatc gaagccagcg acgtcagcgt cgaaggctgc 2701 accctcctca actgggggcg cgatctcacc gccatggatg ccggcgtgca tatcgccgcg //