LOCUS       X07317                  1284 bp    DNA     linear   BCT 31-MAR-1995
DEFINITION  Pseudomonas aeruginosa gene for blue copper protein azurin.
ACCESSION   X07317
VERSION     X07317.1
KEYWORDS    azurin; blue copper protein.
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Lundberg L.
  JOURNAL   Submitted (01-FEB-1988) to the INSDC. Lundberg L., Dept. of
            Biochemistry and Biophysics , Chalmers University of Technology,
            S-412 96 Goeteborg, Sweden.
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Arvidsson R.H.A., Nordling M., Lundberg L.G.
  TITLE     Isolation and Characterization of the Azurin Gene from Pseudomonas
            aeruginosa
  JOURNAL   Eur. J. Biochem. 179(1), 195-200(1989).
   PUBMED   2537198
COMMENT     part of the sequence (pos. 470-942) from strain CIT135 has been
            determined earlier (FEBS Lett. 212, 168-172,1987) showing slight
            differences
            
            Data kindly reviewed (21-APR-1989)
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Pseudomonas aeruginosa"
                     /strain="ATCC 10145"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:287"
     regulatory      317..332
                     /note="promoter P2"
                     /regulatory_class="promoter"
     precursor_RNA   343..983
                     /note="minor transcript (0.54 kb)"
     regulatory      410..445
                     /note="promoter P1"
                     /regulatory_class="promoter"
     precursor_RNA   450..983
                     /note="major transcript (0.65 kb)"
     misc_feature    450..456
                     /note="region of major transcription start"
     regulatory      475..480
                     /note="pot. ribosome binding site"
                     /regulatory_class="ribosome_binding_site"
     CDS             488..934
                     /transl_table=11
                     /note="pre-azurin (AA -20 to 128)"
                     /db_xref="GOA:P00282"
                     /db_xref="InterPro:IPR000923"
                     /db_xref="InterPro:IPR008972"
                     /db_xref="InterPro:IPR014068"
                     /db_xref="InterPro:IPR028871"
                     /db_xref="PDB:1AG0"
                     /db_xref="PDB:1AZN"
                     /db_xref="PDB:1AZR"
                     /db_xref="PDB:1AZU"
                     /db_xref="PDB:1BEX"
                     /db_xref="PDB:1CC3"
                     /db_xref="PDB:1E5Y"
                     /db_xref="PDB:1E5Z"
                     /db_xref="PDB:1E65"
                     /db_xref="PDB:1E67"
                     /db_xref="PDB:1ETJ"
                     /db_xref="PDB:1EZL"
                     /db_xref="PDB:1GR7"
                     /db_xref="PDB:1I53"
                     /db_xref="PDB:1ILS"
                     /db_xref="PDB:1ILU"
                     /db_xref="PDB:1JVL"
                     /db_xref="PDB:1JVO"
                     /db_xref="PDB:1JZE"
                     /db_xref="PDB:1JZF"
                     /db_xref="PDB:1JZG"
                     /db_xref="PDB:1JZH"
                     /db_xref="PDB:1JZI"
                     /db_xref="PDB:1JZJ"
                     /db_xref="PDB:1NZR"
                     /db_xref="PDB:1R1C"
                     /db_xref="PDB:1VLX"
                     /db_xref="PDB:1XB3"
                     /db_xref="PDB:1XB6"
                     /db_xref="PDB:1XB8"
                     /db_xref="PDB:2AZU"
                     /db_xref="PDB:2FNW"
                     /db_xref="PDB:2FT6"
                     /db_xref="PDB:2FT7"
                     /db_xref="PDB:2FT8"
                     /db_xref="PDB:2FTA"
                     /db_xref="PDB:2GHZ"
                     /db_xref="PDB:2GI0"
                     /db_xref="PDB:2HX7"
                     /db_xref="PDB:2HX8"
                     /db_xref="PDB:2HX9"
                     /db_xref="PDB:2HXA"
                     /db_xref="PDB:2I7O"
                     /db_xref="PDB:2I7S"
                     /db_xref="PDB:2IDF"
                     /db_xref="PDB:2IWE"
                     /db_xref="PDB:2OJ1"
                     /db_xref="PDB:2TSA"
                     /db_xref="PDB:2TSB"
                     /db_xref="PDB:2XV0"
                     /db_xref="PDB:2XV2"
                     /db_xref="PDB:2XV3"
                     /db_xref="PDB:3AZU"
                     /db_xref="PDB:3FPY"
                     /db_xref="PDB:3FQ1"
                     /db_xref="PDB:3FQ2"
                     /db_xref="PDB:3FQY"
                     /db_xref="PDB:3FS9"
                     /db_xref="PDB:3FSA"
                     /db_xref="PDB:3FSV"
                     /db_xref="PDB:3FSW"
                     /db_xref="PDB:3FSZ"
                     /db_xref="PDB:3FT0"
                     /db_xref="PDB:3IBO"
                     /db_xref="PDB:3IN0"
                     /db_xref="PDB:3IN2"
                     /db_xref="PDB:3JT2"
                     /db_xref="PDB:3JTB"
                     /db_xref="PDB:3N2J"
                     /db_xref="PDB:3NP3"
                     /db_xref="PDB:3NP4"
                     /db_xref="PDB:3OQR"
                     /db_xref="PDB:3U25"
                     /db_xref="PDB:3UGE"
                     /db_xref="PDB:4AZU"
                     /db_xref="PDB:4BWW"
                     /db_xref="PDB:4HHG"
                     /db_xref="PDB:4HHW"
                     /db_xref="PDB:4HIP"
                     /db_xref="PDB:4HZ1"
                     /db_xref="PDB:4JKN"
                     /db_xref="PDB:4K9J"
                     /db_xref="PDB:4KO5"
                     /db_xref="PDB:4KO6"
                     /db_xref="PDB:4KO7"
                     /db_xref="PDB:4KO9"
                     /db_xref="PDB:4KOB"
                     /db_xref="PDB:4KOC"
                     /db_xref="PDB:4MFH"
                     /db_xref="PDB:4QKT"
                     /db_xref="PDB:4QLW"
                     /db_xref="PDB:4WKX"
                     /db_xref="PDB:5AZU"
                     /db_xref="PDB:5I26"
                     /db_xref="PDB:5I28"
                     /db_xref="PDB:5SYD"
                     /db_xref="PDB:5YT7"
                     /db_xref="PDB:6GYI"
                     /db_xref="PDB:6IAV"
                     /db_xref="PDB:6MJR"
                     /db_xref="PDB:6MJS"
                     /db_xref="PDB:6MJT"
                     /db_xref="UniProtKB/Swiss-Prot:P00282"
                     /protein_id="CAA30279.1"
                     /translation="MLRKLAAVSLLSLLSAPLLAAECSVDIQGNDQMQFNTNAITVDK
                     SCKQFTVNLSHPGNLPKNVMGHNWVLSTAADMQGVVTDGMASGLDKDYLKPDDSRVIA
                     HTKLIGSGEKDSVTFDVSKLKEGEQYMFFCTFPGHSALMKGTLTLK"
     sig_peptide     488..547
                     /note="signal peptide (AA -19 to -1)"
     mat_peptide     548..931
                     /note="mat. azurin (AA 1 - 128)"
     regulatory      961..983
                     /note="put. transcription terminator"
                     /regulatory_class="terminator"
BASE COUNT          230 a          422 c          426 g          206 t
ORIGIN      
        1 ctgcaggctc tgcgggatga tcccgatcac ttcgccgccg cggccaatgc ggcgtccgcc
       61 acggtgccca tcagaccgac cgcgccgcca ccgtagacca gggtcaggcc gcgctcggcc
      121 aggtgccggc cgagggccac ggcggcttcc tggtagaccg gggaagcgcc ggggctggcg
      181 ccacagaata cgcagacgga acgcaaggtc atgatcgact cctgtcgggg gtggaaaaag
      241 gcgcacaggg tagcggctgg gagcgcttcg accaagccgt gcgaagcatt gccggacgtt
      301 gcgtcgcagg cgcgaagcgg cacatctgtg ctaaaacagg agttccccgt agtaaacgcc
      361 gggcagatcc cgctcgatgc cccgccacgt ccggttcggg tttgacctga atcagtggaa
      421 ctcggtgccc gatcgggcag tctgctcttt caggattcat cgcccaacct gcctaggagg
      481 ctgctccatg ctacgtaaac tcgctgcggt atccctgctg tccctgctca gtgcgccgct
      541 gctggctgcc gagtgctcgg tggacatcca gggtaacgac cagatgcagt tcaacaccaa
      601 tgccatcacc gtcgacaaga gctgcaagca gttcaccgtc aacctgtccc accccggcaa
      661 cctgccgaag aacgtcatgg gccacaactg ggtactgagc accgccgccg acatgcaggg
      721 cgtggtcacc gacggcatgg cttccggcct ggacaaggat tacctgaagc ccgacgacag
      781 ccgcgtcatc gcccacacca agctgatcgg ctcgggcgag aaggactcgg tgaccttcga
      841 cgtctccaag ctgaaggaag gcgagcagta catgttcttc tgcaccttcc cgggccactc
      901 cgcgctgatg aagggcaccc tgaccctgaa gtgatgcgcg acgatccgct gcatgaaaaa
      961 gcccggccgc tgccgggctt tttcatgggc gcgcgccggg ctcagcgcgt agcgtgccgc
     1021 catcgcctcg ccggccagtt ggtgcacgcg ccgggtcgga tgccactcgt cccagaagta
     1081 gtactggtcc gggttggcgc aggccgggcg gacgctgggc tgggtcggct ggcagggcgc
     1141 gtccagctcc accaggccat agcgcgccgg gttgcgccgc aagtggcggc tgaaggtgag
     1201 atggtcgaac cagctcagct ccaggccgcg ggtcttgcgc agggcggcga gctggatcgg
     1261 caggctggcg ttgactgcct gcag
//