LOCUS X06402 3941 bp DNA linear SYN 25-AUG-2009
DEFINITION Cloning vector pACYC177.
ACCESSION X06402
VERSION X06402.1
KEYWORDS cloning vector; kanamycin resistance; plasmid.
SOURCE synthetic construct
ORGANISM synthetic construct
other sequences; artificial sequences.
REFERENCE 1 (bases 1 to 3940)
AUTHORS Rose R.E.
JOURNAL Submitted (15-DEC-1987) to the INSDC. Rose R.E., Calgene Inc., 1920
Fifth St., Davis, California 95616, USA.
REFERENCE 2 (bases 1 to 3940)
AUTHORS Rose R.E.
TITLE The nucleotide sequence of pACYC177
JOURNAL Nucleic Acids Res. 16(1), 356-356(1988).
PUBMED 3340534
REFERENCE 3 (bases 1 to 3940)
AUTHORS Mok Y.K.
JOURNAL Submitted (02-MAR-1991) to the INSDC. Mok Y.K., Department of
Biochemistry, Chinese University of Hong Kong, CHINA.
REFERENCE 4
AUTHORS Mok Y.K., Clark D.R., Kam K.M., Shaw P.C.
TITLE BsiY I, a novel thermophilic restriction endonuclease that
recognizes 5' CCNNNNNNNGG 3' and the discovery of a wrongly
sequenced site in pACYC177
JOURNAL Nucleic Acids Res. 19(9), 2321-2323(1991).
PUBMED 2041772
COMMENT The sequence is circular, however the sequence starts with a hincII
site. Bases 564-841, 1494-1721 and 2869-3039 where determined
while the rest of the sequence was taken from the published
sequences of Tn903, Tn3 and p15A.
FEATURES Location/Qualifiers
source 1..3941
/organism="synthetic construct"
/plasmid="pACYC177"
/mol_type="other DNA"
/db_xref="taxon:32630"
misc_feature complement(615..3698)
/note="Tn3 beta-lactamase gene site"
repeat_region 728..765
/note="Tn3 inverted repeat"
misc_feature complement(756..3026)
/note="Tn3 fragment"
rep_origin 766..1595
/note="p15A origin of replication"
misc_difference 1226..1228
/replace="ag"
/note="conflict"
/citation=[1]
/citation=[2]
repeat_region 1590..1817
/note="Tn903 inverted repeat"
misc_feature 1595..3025
/note="HaeII fragment from Tn903"
misc_feature 1922..2734
/note="kanamycin resistance gene"
repeat_region 2798..3025
/note="Tn903 inverted repeat"
misc_feature 3025..3515
/note="Tn3 repressor fragment"
BASE COUNT 1026 a 893 c 993 g 1029 t
ORIGIN
1 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
61 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
121 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
181 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
241 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
301 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
361 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
421 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
481 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
541 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
601 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
661 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
721 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccttaat aagatgatct
781 tcttgagatc gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg
841 cagggcggtt tttcgaaggt tctctgagct accaactctt tgaaccgagg taactggctt
901 ggaggagcgc agtcaccaaa acttgtcctt tcagtttagc cttaaccggc gcatgacttc
961 aagactaact cctctaaatc aattaccagt ggctgctgcc agtggtgctt ttgcatgtct
1021 ttccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg
1081 gggttcgtgc atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg
1141 tggaatgaga caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa
1201 caggagagcg cacgagggag ccgccagggg gaaacgcctg gtatctttat agtcctgtcg
1261 ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc
1321 tatggaaaaa cggctttgcc gcggccctct cacttccctg ttaagtatct tcctggcatc
1381 ttccaggaaa tctccgcccc gttcgtaagc catttccgct cgccgcagtc gaacgaccga
1441 gcgtagcgag tcagtgagcg aggaagcgga atatatcctg tatcacatat tctgctgacg
1501 caccggtgca gccttttttc tcctgccaca tgaagcactt cactgacacc ctcatcagtg
1561 ccaacatagt aagccagtat acactccgct agcgctgagg tctgcctcgt gaagaaggtg
1621 ttgctgactc ataccaggcc tgaatcgccc catcatccag ccagaaagtg agggagccac
1681 ggttgatgag agctttgttg taggtggacc agttggtgat tttgaacttt tgctttgcca
1741 cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc cttcaactca gcaaaagttc
1801 gatttattca acaaagccac gttgtgtctc aaaatctctg atgttacatt gcacaagata
1861 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
1921 ttatgagcca tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg
1981 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
2041 tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
2101 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
2161 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
2221 cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
2281 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
2341 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
2401 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
2461 aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
2521 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
2581 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
2641 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
2701 aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt
2761 aacactggca gagcattacg ctgacttgac gggacggcgg ctttgttgaa taaatcgaac
2821 ttttgctgag ttgaaggatc agatcacgca tcttcccgac aacgcagacc gttccgtggc
2881 aaagcaaaag ttcaaaatca ccaactggtc cacctacaac aaagctctca tcaaccgtgg
2941 ctccctcact ttctggctgg atgatggggc gattcaggcc tggtatgagt cagcaacacc
3001 ttcttcacga ggcagacctc agcgctcaaa gatgcagggg taaaagctaa ccgcatcttt
3061 accgacaagg catccggcag ttcaacagat cgggaagggc tggatttgct gaggatgaag
3121 gtggaggaag gtgatgtcat tctggtgaag aagctcgacc gtcttggccg cgacaccgcc
3181 gacatgatcc aactgataaa agagtttgat gctcagggtg tagcggttcg gtttattgac
3241 gacgggatca gtaccgacgg tgatatgggg caaatggtgg tcaccatcct gtcggctgtg
3301 gcacaggctg aacgccggag gatcctagag cgcacgaatg agggccgaca ggaagcaaag
3361 ctgaaaggaa tcaaatttgg ccgcaggcgt accgtggaca ggaacgtcgt gctgacgctt
3421 catcagaagg gcactggtgc aacggaaatt gctcatcagc tcagtattgc ccgctccacg
3481 gtttataaaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
3541 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
3601 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
3661 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
3721 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
3781 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
3841 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
3901 gcacttttaa agttctgcta tgtggcgcgg tattatcccg t
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