LOCUS V00083 3273 bp DNA circular SYN 17-MAY-1995 DEFINITION Artificial cloning vehicle pBR327, derived from pBR322. The sequence was not resequenced but deduced from the pBR322 sequence. Contains the reading frames for ampicillin resistance (Apr) and tetracycline resistance (Tcr) and an origin of replication. ACCESSION V00083 J02549 VERSION V00083.1 KEYWORDS circular; cloning vector; drug resistance gene; origin of replication; plasmid. SOURCE synthetic construct ORGANISM synthetic construct other sequences; artificial sequences. REFERENCE 1 (bases 1 to 3273) AUTHORS Soberon X., Covarrubias L., Bolivar F. TITLE Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325 JOURNAL Gene 9(3-4), 287-305(1980). PUBMED 6248430 FEATURES Location/Qualifiers source 1..3273 /organism="synthetic construct" /mol_type="other DNA" /db_xref="taxon:32630" CDS 259..1275 /transl_table=11 /note="tetracycline resistance" /protein_id="CAA23425.1" /translation="MRTRSRSTVRPLWPPPSPARFATWSHYRLRDHGDHTRPVDPLRR THRGRHHRRHRCGCWRLYRRHHRWGRSGSPLRAHERLFRRGYGGRPVAGGLLGAISLH APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR ATST" CDS complement(2205..3065) /transl_table=11 /note="ampicillin resistance" /protein_id="CAA23426.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 721 a 911 c 835 g 806 t ORIGIN 1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac 421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca 481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggcccgt ggccggggga 541 ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc 601 aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaccgatg 661 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc 721 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc 781 tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt 841 gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa 901 cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc 961 ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc 1021 ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat 1081 cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat cactggaccg 1141 ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt 1201 gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg 1261 gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc actccaagaa 1321 ttggagccaa tcaattcttg cggagaactg tgaatgcgca aaccaaccct tggcagaaca 1381 tatccatcgc gtccgccatc tccagcagcc gcacgcggcg catctcgggc cgcgttgctg 1441 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 1501 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 1561 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 1621 ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 1681 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 1741 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 1801 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 1861 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 1921 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 1981 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 2041 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 2101 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 2161 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 2221 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 2281 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 2341 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 2401 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 2461 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 2521 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 2581 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 2641 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 2701 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 2761 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 2821 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 2881 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 2941 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 3001 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 3061 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 3121 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 3181 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat 3241 aggcgtatca cgaggccctt tcgtcttcaa gaa //