LOCUS CVU89930 7391 bp DNA circular SYN 01-AUG-1997
DEFINITION Cloning vector pTet-On, complete sequence.
ACCESSION U89930
VERSION U89930.1
KEYWORDS .
SOURCE unidentified cloning vector
ORGANISM unidentified cloning vector
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7391)
AUTHORS Gossen,M. and Bujard,H.
TITLE Tight control of gene expression in mammalian cells by
tetracycline-responsive promoters
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 89 (12), 5547-5551 (1992)
PUBMED 1319065
REFERENCE 2 (bases 1 to 7391)
AUTHORS Gossen,M. and Bujard,H.
TITLE pTet-On (pUHD17-1neo), complete sequence
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 7391)
AUTHORS Holtz,A. and Lou,Y.
TITLE Direct Submission
JOURNAL Submitted (18-FEB-1997) CLONTECH Laboratories, Inc., 1020 East
Meadow Circle, Palo Alto, CA 94303-4230, USA
COMMENT This vector can be obtained from CLONTECH Laboratories, Inc., 1020
East Meadow Circle, Palo Alto, CA 94303-4230, USA. To place an
order, call (415) 424-8222 or (800) 662-2566, extension 1.
International customers, please contact your local distributor or
subsidiary. For technical information, call (415) 424-8222 or (800)
662-2566, extension 3.
This sequence has been compiled from information in the sequence
databases, published literature and other sources, together with
partial sequences obtained by CLONTECH. If you suspect there is an
error in this sequence, please contact CLONTECH's Technical Support
Department at (415) 424-8222 or (800) 662-2566, extension 3 or
E-mail TECH@CLONTECH.COM.
FEATURES Location/Qualifiers
source 1..7391
/organism="unidentified cloning vector"
/mol_type="genomic DNA"
/db_xref="taxon:45196"
misc_feature 774..1781
/note="reverse tetracyclin-responsive transcriptional
activator"
gene 3395..4255
/gene="AmpR"
CDS complement(3395..4255)
/function="ampicillin resistance"
/codon_start=1
/product="beta-lactamase"
/protein_id="AAB64389.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
CDS complement(5668..6462)
/function="neomycin/kanamycin resistance"
/codon_start=1
/product="aminoglycoside-3'-O-phosphotransferase"
/protein_id="AAB64390.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
BASE COUNT 1874 a 1868 c 1835 g 1814 t
ORIGIN
1 ctcgaggagc ttggcccatt gcatacgttg tatccatatc ataatatgta catttatatt
61 ggctcatgtc caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa
121 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
181 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
241 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
301 cgctaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
361 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
421 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
481 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
541 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
601 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
661 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
721 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccgaatt catatgtcta
781 gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg
841 aaggtttaac aacccgtaaa ctcgcccaga agcttggtgt agagcagcct acactgtatt
901 ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc
961 accatactca cttttgccct ttaaaagggg aaagctggca agatttttta cgcaataacg
1021 ctaaaagttt tagatgtgct ttactaagtc atcgcaatgg agcaaaagta cattcagata
1081 cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac
1141 aaggtttttc actagagaac gcgttatatg cactcagcgc tgtggggcat tttactttag
1201 gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta
1261 ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag
1321 agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat
1381 gtgaaagtgg gtccgcgtac agccgcgcgc gtacgaaaaa caattacggg tctaccatcg
1441 agggcctgct cgatctcccg gacgacgacg cccccgaaga ggcggggctg gcggctccgc
1501 gcctgtcctt tctccccgcg ggacacacgc gcagactgtc gacggccccc ccgaccgatg
1561 tcagcctggg ggacgagctc cacttagacg gcgaggacgt ggcgatggcg catgccgacg
1621 cgctagacga tttcgatctg gacatgttgg gggacgggga ttccccgggt ccgggattta
1681 ccccccacga ctccgccccc tacggcgctc tggatatggc cgacttcgag tttgagcaga
1741 tgtttaccga tgcccttgga attgacgagt acggtgggta gggggcgcga ggatccagac
1801 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
1861 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
1921 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
1981 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcctgcaagc
2041 ctcgtcgtct ggccggacca cgctatctgt gcaaggtccc cggacgcgcg ctccatgagc
2101 agagcgcccg ccgccgaggc aagactcggg cggcgccctg cccgtcccac caggtcaaca
2161 ggcggtaacc ggcctcttca tcgggaatgc gcgcgacctt cagcatcgcc ggcatgtccc
2221 ctggcggacg ggaagtatca gctcgaccaa gcttggcgag attttcagga gctaaggaag
2281 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
2341 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
2401 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
2461 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
2521 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
2581 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
2641 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
2701 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
2761 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
2821 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
2881 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
2941 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
3001 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
3061 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
3121 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
3181 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
3241 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
3301 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
3361 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
3421 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
3481 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
3541 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
3601 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
3661 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
3721 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
3781 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
3841 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
3901 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
3961 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
4021 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
4081 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
4141 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
4201 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
4261 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
4321 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
4381 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
4441 cgaggccctt tcgtcttcac tcgaggtcga gggatccaga catgataaga tacattgatg
4501 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
4561 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
4621 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
4681 acctctacaa atgtggtatg gctgattatg atctctagtc aaggcactat acatcaaata
4741 ttccttatta acccctttac aaattaaaaa gctaaaggta cacaattttt gagcatagtt
4801 attaatagca gacactctat gcctgtgtgg agtaagaaaa aacagtatgt tatgattata
4861 actgttatgc ctacttataa aggttacaga atatttttcc ataattttct tgtatagcag
4921 tgcagctttt tcctttgtgg tgtaaatagc aaagcaagca agagttctat tactaaacac
4981 agcatgactc aaaaaactta gcaattctga aggaaagtcc ttggggtctt ctacctttct
5041 cttctttttt ggaggagtag aatgttgaga gtcagcagta gcctcatcat cactagatgg
5101 catttcttct gagcaaaaca ggttttcctc attaaaggca ttccaccact gctcccattc
5161 atcagttcca taggttggaa tctaaaatac acaaacaatt agaatcagta gtttaacaca
5221 ttatacactt aaaaatttta tatttacctt agagctttaa atctctgtag gtagtttgtc
5281 caattatgtc acaccacaga agtaaggttc cttcacaaag atccgggacc aaagcggcca
5341 tcgtgcctcc ccactcctgc agttcggggg catggatgcg cggatagccg ctgctggttt
5401 cctggatgcc gacggatttg cactgccggt agaactccgc gaggtcgtcc agcctcaggc
5461 agcagctgaa ccaactcgcg aggggatcga gcccggggtg ggcgaagaac tccagcatga
5521 gatccccgcg ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc
5581 tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt
5641 cggtcatttc gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc
5701 gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc
5761 gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc
5821 cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt
5881 cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt
5941 gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg
6001 atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg
6061 gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat
6121 ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc
6181 caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac
6241 gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc
6301 ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc
6361 ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca
6421 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc
6481 tgtctcttga tcagatcttg atcccctgcg ccatcagatc cttggcggca agaaagccat
6541 ccagtttact ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccgg
6601 ttcgcttgct gtccataaaa ccgcccagtc tagctatcgc catgtaagcc cactgcaagc
6661 tacctgcttt ctctttgcgc ttgcgttttc ccttgtccag atagcccagt agctgacatt
6721 catccggggt cagcaccgtt tctgcggact ggctttctac gtgttccgct tcctttagca
6781 gcccttgcgc cctgagtgct tgcggcagcg tgttgctagc tttttgcaaa agcctaggcc
6841 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
6901 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
6961 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat
7021 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta
7081 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc
7141 taactgacac acattccaca gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
7201 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
7261 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
7321 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg
7381 agagtgcacc a
//